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NSC-7 could degrade 73.6% of rice straw within 14 d. 10 kinds of material were detected in volatile products by gas chromatography mass spectrometry, and they were mainly acetic acid, glycerol, butyric acid, and propanoic acid. NSC-7 could retain stable degrading ability and high cellulase activity after preserved in -80℃ and freeze-drying for four years. It could remain its degrading ability after 90℃ of high temperature treating for 30 min, and resume its degrading ability after subcultured 2 times after 105℃ of high temperature treating for 30 min in autoclave.

NSC-7在14 d的培养过程中,使稻秆分解73.6%;用GC-MS测定结果发酵液中检测到10种化合物成分,其中峰值较大的依次为乙酸、甘油、丁酸、丙酸; NSC-7在-80℃冷冻和冻干条件下保存4年后仍具有稳定的秸秆分解能力和纤维素内切酶活性;经90℃高温处理30 min后,仍能够保持分解能力, 105℃处理30 min后转接2次就能恢复分解能力,显示出很高的保存稳定性和热稳定性。

NSC-7 could degrade 73.6% of rice straw within 14 d. 10 kinds of material were detected in volatile products by gas chromatog- raphy mass spectrometry, and they were mainly acetic acid, glycerol, butyric acid, and propanoic acid.

NSC-7在14d的培养过程中,使稻秆分解73.6%;用GC-MS测定结果发酵液中检测到10种化合物成分,其中峰值较大的依次为乙酸、甘油、丁酸、丙酸;NSC-7在-80℃冷冻和冻干条件下保存4年后仍具有稳定的秸秆分解能力和纤维素内切酶活性;经90℃高温处理30min后,仍能够保持分解能力,105℃处理30min后转接2次就能恢复分解能力,显示出很高的保存稳定性和热稳定性。

NSC-7 could degrade 73.6%of rice straw within 14d. 10 material were detected in volatile products by gas chromatography mass spectrometry,and it was mainly acetic acid, glycerol, butyric acid, and propanoic acid. NSC-7 could retain stable degrading ability and high cellulase activity after preserved in -80 and freeze℃-drying for four years. It could remain its degrading ability after 90 of high ℃temperature treating for 30min, and resume its degrading ability after subcultured 2 times after 105 ℃of high temperature treating for 30min in autoclave.

NSC-7在14d的培养过程中,使稻秆分解73.6%;用GC-MS测定结果发酵液中检测到10种化合物成分,其中峰值较大的依次为乙酸、甘油、丁酸、丙酸;NSC-7在-80℃冷冻和冻干条件下保存4a后仍具有稳定的秸秆分解能力和纤维素内切酶活性;经90℃高温处理30min后,仍能够保持分解能力,105℃处理30min后转接2次就能恢复分解能力,显示出很高的保存稳定性和热稳定性。

The maximum MTG activity in the culture broth was 5.04u/mL and increased by 21.8% compared with the control. With the addition of CTAB, pro-MTG was activated to become MTG. CTAB could enhance the production of pro-MTG by relieving the pr...

初步研究表明,CTAB的主要作用是促使谷氨酰胺转胺酶的酶原转化为成熟酶,因此,在发酵过程中添加适当浓度的CTAB,可使酶原快速、完全地转化为成熟的MTG,解除酶原的产物抑制作用,促进了细胞产酶。

Other suitable filtration methods can also be employed to remove bacterial cells, for instance, any method of depth filtration with media aids, such as diatomaceous earth.

然后可以通过使培养液通过使用0.1微米过滤器的过滤系统(例如Millipore Prostak过滤系统)收集发酵培养基中的植物蛋白A。

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