使加入

There is usually a 3rd -key added in the opening of the second theme, and sometimes a new theme is composed. Then, the subordinate key is reached before the end of exposition. 5 Silence of one whole bar is frequently utilized, which not only brings profound expressive significance, but also is important to the architectonics of these sonatas. 6 The harmonization in the variation, which is the method of applying different keys to the same melody, is handled in those Andante movements. By this way, the same melody appears in variable colors of harmony, which can not only avoid dull repetition, but also keep the lyrical nature and the completeness of melody. 7 Chromatic method is applied in the periods of development, and chromatic深层线条becomes the provident feature in some periods of development. 8 Atlas motive is put to use in c minor and A major sonatas, and a lot of "sigh" tones and c sharp minor are both preferred in those three sonatas. 9 The emotional factor of "wanderer" is the roots of thoughts for the intense conflict of music.

他通常在副部的开始加入一个三度的调性，有时再加入一个新主题，最后在呈示部结束之前到达属调； 5、较多地运用整小节的休止，使其不仅具有深刻的表情意义，而且在结构乐曲中起到了不可或缺的作用； 6、舒伯特在慢板乐章中运用同一旋律异调配置的和声变奏方式来构成音乐的段落，使同一个旋律在不同的和声色彩里闪现，既避免了单调的重复，又保持了旋律的抒情性和完整性； 7、展开的段落中习惯运用半音化的发展手法，半音阶式的深层线条成为某些展开段落的显著特点； 8、c小调和A大调奏鸣曲中都运用了"阿特拉斯"动机，三首奏鸣曲中运用了大量叹息的音调，并倾向于~#c小调的运用； 9、"流浪者"的情感因素是音乐强烈冲突的深刻思想根源。

Changes of silver carp muscle protein s during chilled storage;2. The changes of rheology,texture and color of minced pork after the cooking respectively in water bath,retort and microwave were tested,based on which the effects of muscle protein and transglutaminase contents on the gel properties of minced pork were then investigated.

结果表明：3种热处理方法下，一定量的转谷氨酰胺酶及肌肉蛋白使猪肉糜表现出不同的颜色和质构特性；与其他两种方法相比，水浴加热使猪肉糜更具有弹性；加入MTGase后，样品的储能模量G′增大，表明MTGase的加入影响了猪肉糜的流变特性、颜色以及质构，其效力取决于猪肉糜的热处理方法。

Process of flotation of acture ore is the following: firstly, adding Na_2CO_3, ZnSO_4, CSU-1 to the pulp while grinding, and thus in this condition of low Eh, marmatite is inhibited; Secondly, adding CSU-1 to the pulp out of grinding machine, then amesonite and iron sulfur mineral can be flotated using diethyldithiocarbamate as collector; High quality lead and antimony concentrate can be got by adding CSU-1 and calcareousness; Lastly, marmatite is flotated by adding calcareousness, CuSO_4, xanthe, and D-250 to get zinc concentrate.

小型试验研究表明，首先在脱硫粗选作业只加入适量的Na_2CO_3、ZnSOO_4、和CSU-1至磨矿机，利用磨矿机的研磨和磨机中的低电位环境，使铁闪锌矿受到抑制，而最容易浮的脆硫锑铅矿和黄铁矿、磁黄铁矿等硫化矿物则依然具有可浮性；在磨机出来的矿浆中分别加入CSU-1进一步抑制易浮的铁闪锌矿，然后加入乙硫氮将脆硫锑铅矿和硫化铁矿快速浮净，加入石灰和CSU-1，浮选分离出高质量的铅锑精矿；接着添加石灰、CuSO_4、黄药及2~#油选别铁闪锌矿。

The reason may be that complex formed by rhodium and halide was difficult to produce the catalytic active species. Therefore halide ion was the poison for the rhodium catalyst in hydroformylation of olefins in ionic liquid. The effect of double long chain cationic surfactant (DCMAB, dicetyl dimethylammonium bromide) on selective hydrogenation of cinnmaldehyde catalyzed with RuCl3·x H2O/TPPTS was studied.

进一步的研究表明在使用有良好反应活性的两种催化剂HRh3 、Rh_2进行催化反应时，向体系中加入含有卤离子的化合物时将使反应活性大大降低，而且随着卤化物加入量的增加，催化剂的活性迅速下降，这是卤离子可能和金属铑作用使它难于生成催化活性物种，进一步证明卤素离子是引起催化剂活性下降的极其重要的因素。

Therefore can pass to use the cupric sulfate sulfuric acid potassium for the catalyst take sulfuric acid as to carry the body to digest it, make the amino acids chelate match the conversion for dissolve sex easily of ammonium sulfate and white vitriol, then absorb a fixed amount to try the kind to join 40% sodium hydroside combine under the steam heat function make ammonia the spirit swims to leave out, through cool off to respond the born boric acid with boric acid, be got the total nitrogen content by the standard aqua titration of hydrochloric acid immediately after;But the white vitriol can immediately join the hydrochloric acid hydroxylate , fluoride to turn the in order to cover up, carry on the titration but get the content of its zinc with the EDTA mark liquid..

因此可以通过用硫酸铜硫酸钾为催化剂以硫酸为载体将其消化，使氨基酸螯合锌转化为易溶性的硫酸铵和硫酸锌，然后吸取一定量的试样加入40 %的氢氧化钠并在水蒸气高温作用下使氨气游离出来，经冷却与硼酸反应生成硼酸铵，接着由盐酸标准溶液滴定得到总氮含量；而硫酸锌即可加入盐酸羟铵、氟化氢铵为掩蔽剂，用EDTA标液进行滴定而得到其锌的含量。

When reached logarithm growth phase, it was poured or the medium was removed. 5 mL medium containing 10 mg/L mitocin-C was added , kept at 37 ℃, and then cultured in saturated humidity warm box with the CO2 of 0.05 fraction volume for 2 or 3 hours. It was coated with 0.1% gelatin in 6-hole plate, laid for over 30 minutes, and then threw away or the medium with mitocin-C was removed, washed with phosptat buffer for 5 times so as to remove the mitocin-C. 2 mL 0.05% trypsin digestive cells were added, and it was observed under microscope. When crevice appeared, cells became round (about 2－4 minutes), digestion was stopped by adding medium of the same volume, and blew up with straws repetitively to make it into monoplast suspension. Special-used cover glass was put in the center of cell counting chamber. Cells were sucked in by glass siphon, and cell suspension flew out at bucket of up or down-sides of counting chamber, until the cover glass was filled with fluid. Living cell were inoculated at concentrations of 3×108, 5×108, 1×109 L－1 after their number counted.

选取2～5代的小鼠胚胎成纤维细胞，待其至对数生长期倒掉或吸掉培养基，加入含10 mg/L丝裂霉素C的细胞全培养液5 mL，置37 ℃，体积分数0.05的CO2饱和湿度温箱中培养二三小时，在6孔板中加入0.1%明胶包被，放置30 min以上，倒掉或吸掉含丝裂霉素C的培养基，磷酸盐缓冲液清洗5遍，尽量除去丝裂霉素C，加入2 mL 0.05%的胰蛋白酶消化细胞，镜下观察，当细胞间出现裂隙，细胞变圆时（约2～4 min），立即加入等量的全培养液终止消化，并用吸管反复吹打，使之成为单细胞悬液，在细胞计数板中央放置专用的盖玻片，用玻璃虹吸管吸取细胞，让虹吸管在盖玻片上或下侧的计数板凹槽处流出悬液，至盖玻片下被液体充满为止。

The results showed the addition of PPS did not decrease the thermal performance but decreases the heat deformation temperature of PPES/PPS blends. The crystallinity of PPS was hindered with the increasing of PPES contents, the crystal lattice flaws of PPS occurred and the perfect spherocrystal could not appear when the mass fraction of PPES was 80%. The annealing process was favorable to the increase of the heat deformation temperature and the formation of perfect spherocrystal of PPS.

热失重研究表明，在所组成范围内PPS的加入并未降低共混物的热性能，但使共混物的热变形温度有所降低；PPES的加入使PPS的结晶受到阻碍，当PPES质量分数达80%时，PPS产生了晶格缺陷，从而不能形成完善的球晶，退火过程有利於提高共混物的热变形温度，使PPS形成更完善的球晶。

Part II The experiment study on anti-tumour effect of dendritic cells derived from peripheral blood monocyte of patients with oral cancer after pulsed by tumour antigen in different ways. Collected 50ml peripheral blood from each patients and volunteers to induced DC in vitro. We parted the test group and control group into three subgroups respectively, untreated group A, freezed and thawn group B, RNA transfected group C. To group A, after induced DCs for five days , we added 200U/mlTNF-α in each hole and cultured on. To group B, we added protein antigen (DC/ tumor cells = 1 : 1 ,each hole) for 4h at 5d, and then added 200U/mlTNF-α in each hole and cultured on. To group C, transfected immature DC with tumor cell RNA(DC:RNA=1×105/ml: 5ug)at 5d, operated completely with the specification of invitrogen DMRIE-C reagents. After transfection we replaced transfection agent with complete medium, and then added 200U/mlTNF-α in each hole and cultured on.

第二部分口腔癌患者外周血来源的树突状细胞体外负载抗原后抑瘤作用的实验研究无菌采集20例口腔癌患者和20例健康志愿者外周血50ml，分离单个核细胞体外诱导培养DC，并将实验组和对照组细胞各分为三组，A组为未处理组，B组为冻融组，C组为RNA转染组。A组DC在培养5d后，每孔加入TNF-α200U/ml；B组DC培养第5d后，每孔中加入癌细胞蛋白抗原，使DC/肿瘤细胞=1：1，继续培养4h后，每孔加入TNF-α200U/ml；C组将培养至第5d的未成熟DC用肿瘤细胞RNA进行转染(DC：RNA=1×105/ml： 5ug，按照invitrogen DMRIE-C试剂说明书进行操作)，转染结束后用完全培基取代转染剂，每孔加入TNF-α200U/ml。

The experimental results are summarized as follows: the tensile strengh and bending strengh of steel fiber reinforced lightweight aggregate concrete can be improved significantly by the addition of steel fiber; to some extent ,the addition of steel fiber can also increase the compressive strength ,although little , it radically improves the compressive toughness , and the steel fiber reinforced lightweight aggregate concrete will not fall apart with cracks; with the increase of the volume fraction of steel fiber, the elastic modulus is also rising somewhat; Poisson's ratio is influenced little with the addition of the steel fiber and the effect regularity displays unconspicuously.

本文试验研究结果可归纳如下：钢纤维的加入能明显提高钢纤维轻骨料混凝土的抗拉强度和抗折强度；掺入钢纤维使钢纤维轻骨料混凝土的抗压强度有一定程度的提高，增长幅度不大，但抗压韧性却有很大改善，使钢纤维轻骨料混凝土裂而不散；钢纤维轻骨料混凝土的弹性模量随钢纤维体积率的增大有一定提高；泊松比受钢纤维的加入影响不大，规律性不明显。

In this study,the muscarinic current was induced by carbachol(50 umol/ L) or GTPYB(0.5 mmol/L).The results showed that hyposmotic superfusate(202mOsmol/ L) increased carbachol-induced current by 145士27%and increased GTPYS-induced current by 183士30%;but in the presence of cytochalasirr B(cyt- B,20 umol/ L),an actin cytoskeleton disrupter,posmotic membrane stretch increased 1CC6 by 70士6%.However,hypos motic membrane tretch induced increase in 1CC6 was potentiated to 545士81% by phalloidin(20 umol/ L),an actin microfilament stabilizer.

当豚鼠胃窦平滑肌细胞的膜电位钳制在一20 m V时，灌流液中50umol/ L卡巴胆碱(carbachol ， CCh)或电极内液中0.5 mmol/L GTPYS均可引导毒覃碱电流，2 m Os mol/ L分别使其增加145士27%和183士30；当电极内液中加入20 umol/ L的细胞松弛B时，低渗牵张使1 cce只增加70士6；而电极内液中加入20 umol/ L的鬼笔环肤则使Icce增加了545士81。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。