使分离的

Part II The experiment study on anti-tumour effect of dendritic cells derived from peripheral blood monocyte of patients with oral cancer after pulsed by tumour antigen in different ways. Collected 50ml peripheral blood from each patients and volunteers to induced DC in vitro. We parted the test group and control group into three subgroups respectively, untreated group A, freezed and thawn group B, RNA transfected group C. To group A, after induced DCs for five days , we added 200U/mlTNF-α in each hole and cultured on. To group B, we added protein antigen (DC/ tumor cells = 1 : 1 ,each hole) for 4h at 5d, and then added 200U/mlTNF-α in each hole and cultured on. To group C, transfected immature DC with tumor cell RNA(DC:RNA=1×105/ml: 5ug)at 5d, operated completely with the specification of invitrogen DMRIE-C reagents. After transfection we replaced transfection agent with complete medium, and then added 200U/mlTNF-α in each hole and cultured on.

第二部分口腔癌患者外周血来源的树突状细胞体外负载抗原后抑瘤作用的实验研究无菌采集20例口腔癌患者和20例健康志愿者外周血50ml，分离单个核细胞体外诱导培养DC，并将实验组和对照组细胞各分为三组，A组为未处理组，B组为冻融组，C组为RNA转染组。A组DC在培养5d后，每孔加入TNF-α200U/ml；B组DC培养第5d后，每孔中加入癌细胞蛋白抗原，使DC/肿瘤细胞=1：1，继续培养4h后，每孔加入TNF-α200U/ml；C组将培养至第5d的未成熟DC用肿瘤细胞RNA进行转染(DC：RNA=1×105/ml： 5ug，按照invitrogen DMRIE-C试剂说明书进行操作)，转染结束后用完全培基取代转染剂，每孔加入TNF-α200U/ml。

Re-W alloy is oxidized and separated through air lancing reaction in a tubular oxidizing furnace with different temperature zones, and the Re oxide is heated and reduced in H2 atmosphere to obtain high purity Re powder.

将钨铼合金在具有不同温区的氧化气氛管式炉中进行吹扫反应，同时对不同区域温度、吹扫气体流量、反应系统出口气流量进行集中控制，以便在合适的气氛与温度下使钨铼合金充分氧化，并得到有效分离，最终对铼的氧化物在氢气氛中加热还原，制得高纯铼粉。

We design a light metallic system which permits ample overhangs, freeing up the interior space from other structural elements or walls.

设计者为其设计了高悬的轻的金属系统，使其内部空间和其他的结构以及墙体分离。

Players that separete themselves from any piece of equipment or clothing that they brought onto the game field by more than2 meters, except squeegees or pods in general used for holding paintballs, will be immediately eliminated.

翻译的意思为，参赛者将携带至场地内的任何装备或衣著分离超过2公尺将立即被判阵亡，枪管清洁条或使用于装载漆弹球的弹桶除外。

But when making positive pole with silicon film or grain when researcher, a large number of coming back the lithium atom that leave can destroy these silicon, make at the same time its and the metal below base piece detached, reduced the effect of batteries finally.

但是当研究人员用硅薄膜或颗粒制成阳极时，大量旋离的锂原子会摧毁这些硅，同时使其与下面的金属基片分离，最终降低了电池的功效。

Tissue culture plate was proved to be excellent to provide convenient microscopic observation and suitable space for microalgal living.

利用组织培养板方便的显微观测条件和适宜容积提供微藻存活的空间，在不同培养条件下，进行微藻群落演替、及时显微跟踪检测和优势种分离，可以使野外水样初期低生物量种类优势化，以利于纯系种质的获得。

Gios PDF Splitter and Merger is a PDF tool developed for quicker and more efficient access to information needed from your PDF files, reorganizing them in a way that makes them easier to ...

Gios PDF格式分离器和合并是一个PDF工具更快和更有效地访问您的PDF文件所需要的信息开发，重组的方式，使他们更容易对他们。。。

He was a major contributor to The distance teaching universities (1982), joint editor of Distance education: international perspectives (1983), joint editor of L'università a distanza. Riflessioni e proposte per un nuovo modello di università(1984), editor of Theoretical principles of distance education.(1993), joint editor of Distance education: new perspectives (1993), editor of The industrialization of teaching and learning (1994). In 1997 the European Commission in Brussels published his Distance training in the European Union .

在整个学习过程期间，教师和学生处于准永久性分离状态（以此与常规面授教育相区别）；教育组织在材料计划、准备和学生支持服务准备两方面的影响（以此与个别学习和自教计划相区别）；技术媒体（印刷媒体、视听媒体或计算机媒体）作为课程内容的载体，把教师与学生联系起来；提供双向通信，使学生可以主动对话并从对话中受益（以此与教育技术的其他应用相区别

With a large amount of moistures in the crude oil that exploit just, so not in crude oil go on and add medicine control to their when entering the station, Make the profit can be separated , make the quality of the crude oil improve.

刚开采的原油中均含有大量的水分，油田在原油外输前，原油先送到联合站处理，处理后在统一外输。原油进站时对其进行加药处理，使油水能够分离，是联合站原油处理的工艺之一。

Roller to overcome the internal friction and innovativeink to do work, and make the roller surface temperatures, passed and was well received in the course of extrusion, shear.

辊子为克服油墨的内摩擦力而作功，而使墨辊表面温度升高，被传递的和被打匀的过程中，受到挤压、剪切而分离。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。