使减弱

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

Ach has a consanguineous relation with the people's attention, cognitive functions and the behavior ability.

脑神经中的Ach水平降低而使Ach神经传导损伤与AD病人的认知能力丧失及行动能力减弱等痴呆症状有关，这些新的研究进展，为发展治疗AD疾病的拟胆碱能药物提供了强有力的证据；2。

Increasing the concentration of Cl〓 heightened the corroding rate of carbon steel in the solutions with binary inhibitors.

失重法研究证明，镁离子的存在使钨酸盐的缓蚀作用减弱。

Methods: According to the degree of color change, the ozone in the ambient air causes the indigo disulfonic acid sodium discoloration response a new colorless production.

空气中的臭氧使吸收液中蓝色的靛蓝二磺酸钠褪色，生成无色靛红二磺酸钠，根据颜色减弱程度比色定量。

Furthermore, knockdown of Hspa5 rescues ventralized phenotypes induced by overexpression of Bmp2b; on the other hand, dorsalized phenotypes caused by Hspa5 knockdown disappear when Bmp2b mRNA is coinjected. It is likely that involvement of Hspa5 in dorsoventral patterning is associated with Bmp signals.

进一步研究发现，抑制Hspa5的表达能够挽救Bmp2b过量表达所导致的胚胎腹部化，Bmp2b的过量表达可使阻断Hspa5表达所引起的背部化表型减弱，提示Hspa5在胚胎背腹分化方面的作用可能与Bmp信号相关。

AOPA, by "multiple relevant convergence reaction", adjusts and affects systematically the endocrinal function of bone metabolism,improves the levels of estrogen and CT/PTH, reverses the declining tendency of pituitary-thyroid axis function and improves thyroid function so as to weaken osteocyte in the bony absorption, enhance osteoblast in osteogenesis and increase bone density.

AOAP可以通过&多位点相关集合效应&综合调控影响骨代谢的内分泌功能，提高雌激素、降钙素/甲状旁腺素水平，扭转垂体-甲状腺轴功能减退的趋势，改善甲状腺的功能，使破骨细胞的骨吸收减弱，成骨细胞的骨形成增强，增加骨密度。

The researchers have confirmed, in a wide range of cells, that nanoparticles are taken into a region called the endosome, where this essential coating is degraded by cathepsin L.

赛尔博士表示，利用纳米粒子作为药物传递工具是很有前途的应用，但要使这样的生物疗法奏效，粒子外层蛋白就要保持高度完整，但组织蛋白酶L的降解作用将会致使药效减弱。

The carcinoma of esophago lymph vessel in periphery areas provid conditions for cancer cell to shift.

e-cadherin、β-catenin在食管癌细胞中的表达减少，推测使食管癌细胞间的粘附能力减弱，促进了癌细胞的转移。

Newborn hiccup caused by the three, first, as a result of improper care, exopathic cold, cold and heat and tend to reverse the air, As the saying goes, is "drinking the air-conditioning" and induced hiccup; Second, as a result of improper eating milk, fresh milk, if not controlled , the plot is not stopped, or have milk or too cold food served cold medicines are not qi, spleen and stomach function, abnormal movements of Qi Wei Qi move上逆hiccup induced diaphragm; Third, as a result of eating too fast or scared crying after eating, can also be induced by temporary hiccup choke.

新生儿打嗝多由三方原因引起，一是由于护理不当，外感风寒，寒热之气逆而不顺，俗话说是&喝了冷气&而诱发打嗝；二是由于乳食不当，若乳食不节制，停积不化，或过食生冷奶水或过服寒凉药物则气滞不行，脾胃功能减弱，气机升降失常而使胃气上逆动膈而诱发打嗝；三是由于进食过急或惊哭之后进食，一时哽噎也可诱发打嗝。

Comparative studies of electroactive components and voltammetric behaviors different between the MCF-7 cell without estrogen and the MCF-7 cell with estrogen showed that the concentration of the guanine and xanthine bases in the cytoplasm decreased, which resulted in the decrease of secretion ability of the MCF-7 cell without estrogen decreased, and these result in the decreasing of the voltammetric response of the cells, impling that the cell vaibility decreases.

结果发现，去激素MCF-7细胞质中的黄嘌呤和鸟嘌呤浓度降低，使细胞分泌黄嘌呤和鸟嘌呤能力降低，导致细胞和细胞质的伏安信号减弱，说明去激素MCF-7细胞的活性下降；研究了标准雌激素雌二醇、环境雌激素壬基酚和双酚A对去激素培养的MCF-7细胞增殖的影响，发现其均存在剂量-效应关系和时间-效应关系，并且雌激素效应由大到小的顺序为：雌二醇＞壬基酚＞双酚A；同时使用3-(4，5-Dimethylthiazol-2-yl)-2，5-diphenyltetrazolium bromide法验证了电化学法评价雌激素效应的可靠性。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。