使免疫

The herpes viruses spur the immune system to boost levels of a protein hormone called interferon gamma "that in effect puts some immune system soldiers on yellow alert, causing them to patrol for invaders with their eyes wide open and defense weapons ready," Virgin said.

疱疹病毒刺激免疫系统使一种称为干扰素γ的蛋白质激素的水平提高，"那实际上就是向免疫系统士兵拉响空袭警报，唤起他们睁大眼睛和准备好防御武器去巡视侵入者，"Virgin说道。

The herpes iruses spur the immune system to boost leels of a protein hormone called interferon gamma "that in effect puts some immune system soldiers on yellow alert, causing them to patrol for inaders with their eyes wide open and defense weapons ready," irgin said.

疱疹病毒刺激免疫系统使一种称为干扰素γ的蛋白质激素的水平提高，"那实际上就是向免疫系统士兵拉响空袭警报，唤起他们睁大眼睛和准备好防御武器去巡视侵入者，"irgin说道。

TJU103 combined with CTLA4-Ig could induce tolerance of donor CD4〓 T-cell to the given antigen, and the tolerance could be transferred by the anergic CD4〓 T-cell and supernatants.

TJU103联合CTLA4-Ig应用，能够调节Th细胞分化，诱导Th细胞发生免疫偏离，使辅助性T细胞更多地向Th2细胞方向分化，有利于免疫耐受的形成。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

VSG is commonly considered a strong immunogen and only enables the immune protection to homotype rather than heterotype.

VSG 是公认的强免疫原，但只能使机体产生针对同型虫体的免疫保护，而对异型虫体不起保护作用。

A new eucaryotic expression system of recombinant PoIFN-γ was also constructed so that the denaturalization and refolding process is avoided. The target gene coding PoIFN-γ2 mature protein was subcloned into expression vector, transformed into Pichia pastoris, The His〓 Mut〓 phenotype transformants were screened, fermented in flasks and induced by 1％methanol. The expressed product in culture solution and sedimentation have antiviral activity on VSV and immunological activity proved by indirected immunofluorescence antibody and Dot blotting assay.

为了构建可对表达产物修饰、加工的真核表达系统，使表达产物具有自然干扰素的活性，无需变性、复性，我们将PoIFN-γ2成熟蛋白基因连接酵母整合表达载体，电转化毕赤酵母，筛选了His〓Mut〓表型转化子，摇瓶培养，1％甲醇诱导表达，培养上清和菌体裂解上清均有抗VSV病毒活性，经间接免疫荧光抗体检测和Dot blotting鉴定，重组酵母菌诱导表达产物具有免疫活性。

In other words, we prove our working hypothesis related to immune hyper-activation within intestinal mucose during HIV infection by the studies.

而在HIV感染时，由于危险信号的释放，病毒子模拟APc的作用，调节性T细胞优先被删除等原因导致生理性低反应性状态被打破，而出现免疫过度活化，从而产生更多的HIV易感细胞，通过直接感染以及活化诱导细胞凋亡，使肠道T细胞在短期内大量丢失，从而导致免疫缺陷。

Investigation showed aquaporin-4 played an important role in humoral-mediated immunologic mechanism of neuromyelitis optica.Meanwhile,NMO revealed an autoimmune channel disease,and the study on the immunopathogenesis of NMO was ushered into a new stage.

目前研究发现AQP4还参与视神经脊髓炎的体液免疫机制，揭示了NMO是一种自身免疫性通道病，这一发现使NMO的免疫和病理学研究进入了一个新的阶段。

Result:It was showed that Zhechong not only lowered markedly whole blood viscosity,plasmatic viscosity and packed cell volume but also promoted red cell immune function,which may be:①RBC C3b receptor rosette rate(RCR1R) could be enhanced,②RBC immune complexes rosette rate could be reduced,③the serum rosette forming enhancing rate of RBC C3b receptor could be increased,④the serum rosette forming inhibitory rate of RBC C3b receptor could be decreased.Moreover,there were the inverse correlation between blood viscositv and RCR1R.

结果：虫能明显降低瘀血大鼠的全血比粘度、血浆比粘度和红细胞压积；提高瘀血大鼠红细胞C3b受体花环率，使红细胞免疫复合物花环率下降，血清红细胞免疫粘附促进因子活性增强，而抑制因子减弱；血液粘度与红细胞C3b受体花环率的相关分析呈显著负相关。

We found that both SL7207 and SL7207/pEGFP-N1 vaccines can not induce theprotective immunity,while SL7207/pGFP-FL vaccines can trigger antitumorresponses in partial (2/10) of orally immunized mice.And these two tumor-free micealso got the immunity to rechallenge the 10-fold dose of H1 tumor cells when given40 days post the primary challenge.

我们发现，野生型的SL7207和SL7207/pEGFP-N1均不能诱发小鼠的保护性免疫，而SL7207/pGFP-FL则可以在部分小鼠（2/10）中诱发保护性免疫，使小鼠免生肿瘤，并能抵抗在初次荷瘤攻击后40天，10倍于首次剂量的H1肿瘤细胞的再攻击。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。