使...影响

We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果：体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；硒能增强重组VacA对胃癌细胞的抑制作用，使细胞分裂指数和集落形成率降低、S期细胞数量减少，G1期细胞总数增加；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

The article mainly analyzes how innovation affects the social welfare and makes some conclusions as follows: firstly, influences on the price and output of products of innovation by oneself are uncertain, but innovation can enhance social welfare when it reduces the product price or raises the price but diminishes the elasticity of inverse demand curve.

本文重点分析了企业创新对社会福利的影响，得出的主要结论如下：（1）独立创新对产品价格和产量的影响是不确定的，但当创新使产品价格下降或者创新使产品价格上升且使反需求曲线的弹性变小时，独立创新能够增加社会福利；（2）联合创新能够降低每个企业的成本，增加企业的研发开支、产量和利润，因而联合创新有利于社会福利；（3）在考虑了创新的溢出效应后，存在一个外溢比率的帕累托最优区间，但我们必须在创新激励与社会福利之间做出权衡。

Dealcoholization temperature markedly influences the surface area and porosity of the resultant MAO-supports. When the support was pretreated at lower or higher temperature, the corresponding MAO-support shows very low surface area;2.XRD and IR analyses indicate that MAO has reacted with the residual ethanol in the supports, which is an important manner to get MAO fixed on the carrier. Morever, XPS analyse indicates that MAO can also be coordinated with MgCl_2, which is another way to get MAO fixed on the support;3.ICP and EDX analyses indicate that dealcoholization temperature not only determined the element content on the solid catalyst, but also influences the element distribution on the carrier;4.The activities of the supported phenoxy-imine catalysts and the properties of resultant polymers are strongly dependent on the dealcoholization temperature. The support (MSP-5) obtained by treating MgCl_2·2.56C_2H_5OH at 160℃for 4 h, then modified by MAO is very effective for immobilizing complex 3, the resultant solid catalyst (MSPC-5) shows very high activity in ethylene polymerization, and its kinetics of polymerization is stable during the reaction process. Finally, PEs with spherical morphology and high bulk density (over 0.35g/ml) were obtained, without reactor fouling;5.In this work, polymerization conditions such as alkylaluminums, Al/Zr ratio, temperature and H_2 had a pronounced effect on the activity of MSPC-5 and properties of PE;(3). New MgCl_2-Supported Single-Site Catalysts for Ethylene PolymerizationIn this work, a kind of new MgCl_2 support was obtained by anhydrous MgCl_2 co-milled with solid MAO, and it is a creative contribution. Then some single-site catalysts were supported on the new MgCl_2 support, and the resulted solid catalysts were tested in ethylene polymerization, the results indicated that:1.XRD and IR analyses indicate that MAO does be coordinated with MgCl_2, which is in good agreement with the results obtained by XPS;2.co-mlling time had no obvious effect on the texture of support after 12 hours;3.In this work, MgCl_2 was co-milled with solid MAO for different hours, and then complex 3 was supported on these co-milled supports. It was found that co-milling time markedly influences the activity of solid catalysts, but it had negligible effect on the kinetic profile and the properties of resultant PE.

XPS研究结果表明，MAO不仅仅只和乙醇作用，MAO还与MgCl_2本身有一定的作用，这是一个极为重要的发现；3、通过ICP和EDX表征，发现载体的脱醇温度直接影响着元素在固体催化剂中的含量及分布；4、脱醇温度极大地影响着负载苯氧基亚胺类催化剂的活性和聚合物的性能，特别是原始载体经160℃活化4小时制备得到的固体催化剂MSPC-5的活性明显高于其他脱醇温度条件下制得的固体催化，并且催化剂寿命长，表现出稳定的动力学行为，最终获得了高堆密度(大于0.35g／ml)的球形聚乙烯颗粒；5、本文选择MSPC-5为研究对象，考察了聚合反应参数对催化剂的活性及其聚合物的影响，研究发现：不同的烷基铝对催化剂具有非常重要的影响，特别足TIBA对MSPC-5的助催化活性最高，烷基铝的加入量有一个较佳值，聚合温度为80度时活性最高，H_2的加入使催化剂的活性明显降低且聚合物的分子量也减小；、新型氯化镁载体负载单活性中心催化剂催化乙烯聚合本文创造性采用固体MAO与无水氯化镁直接进行共研磨，制备出了一种的新型的氯化镁载体，并将该载体用于多种单活性中心催化剂的负载化研究，研究表明：1、通过BET、XRD的表征，再次证实了MAO与MgCl_2之间具有直接作用，与XPS的研究结果相一致；2、当研磨时间高于12小时时，延长研磨时间对载体的结构没有显著的影响；3、将配合物3负载于共研磨时间不同的一系列载体上，乙烯聚合结果表明，尽管共研磨时间对负载催化剂的活性具有明显的影响，但是它对聚合物的性能以及催化剂的影响不明显。

Results are as followed:1 Exposure of HELF cells to BP caused c-Jun activation,and increased the activity of MAPK,PI-3K,p53 and cyclin D1 pathway.2 BP-induced c-Jun activation was inhibited by dominant negative mutants of extracellular signal-regulated protein kinase or c-Jun NH_2-terminal kinase,but not by p38,impling that JNK and ERK pathways medicate c-Jun activation induced by BP.3 Overexpression of dominant-negative mutants PI-3K and Akt potently blocked phosphorylations of c-Jun and ERK,but not JNK in response to BP,suggesting that PI-3K/Akt pathway positively regulates BP-induced c-Jun activation through ERK.4 Inhibition of p53 by its chemical or molecular inhibitor markedly increased the phosphorylation levels of c-Jun,Akt and ERK upon BP stimulation,indicating that p53 negatively medicates BP-induced c-Jun activation through PI-3K/Akt/ERK pathway.5 The cell lines expressed TAM67 exhibits no significant affecting normal cell growth properties.6 TAM67 was able to significantly block G_1-S transition and subsequent cell proliferation,suggesting that c-Jun is essential for cell cycle alternations elicited by BP.7 Overexpression of TAM67 impaired BP-induced cyclin D1 activation,decreasing expression of E2F1 and pRb,indicating that c-Jun participates in the modulation of BP-induced activation of cyclin D1/pRb/E2F1 pathway.8 Stably expression of TAM67 led to the increases in the expression levels of p53 and p21,elevating phosphorylation level of p53,clearly indicating that c-Jun regulates p53/p21 pathway activation induced by BRCollectively,PI3K/Akt/ERK pathway mediated BP-induced c-Jun activation through p53-dependent mechanism.

结果显示：1BP刺激细胞可促进c-Jun活化，并伴随着MAPK、PI-3K、p53和cyclinD1通路各组成成分的活性增强。2利用MAPK通路的显性失活突变体分别阻断细胞外信号调节激酶和c-Jun氨基末端激酶活性，均可明显抑制BP诱导的c-Jun活化，但阻断p38活性对BP引起的c-Jun活化无明显影响，提示JNK和ERK通路参与调控BP诱导的c-Jun活化。3过表达PI-3K和Akt的显性失活突变体也可显著抑制BP诱导的c-Jun活化，并降低磷酸化ERK的表达水平，但对磷酸化JNK的表达水平无明显影响，说明PI-3K/Akt通路通过ERK正性调控了BP诱导的c-Jun活化。4p53的化学/分子抑制剂能使BP作用的细胞内c-Jun活性明显增加，并同时诱导Akt和ERK的磷酸化水平的升高，表明p53可通过PI-3K/Akt/ERK通路对BP诱导的c-Jun活化进行负性调控。5随后观察转染细胞的生长情况，发现TAM67对细胞正常生长和形态无明显影响。6稳定表达TAM67可有效抑制BP诱导的S期细胞数的增加，提示c-Jun在BP致细胞周期改变的过程中发挥了重要作用。7TAM67过表达能够抑制BP诱导的cyclin D1活化，降低磷酸化Rb以及E2F1蛋白表达水平，表明c-Jun参与调控BP诱导的cyclin D1/Rb/E2F1通路的活化。8过表达TAM67可使BP刺激的细胞中p53、p21总蛋白以及p53磷酸化的表达水平明显升高，可见c-Jun也参与调控BP诱导的p53/p21通路活化。

The main fatty acid from longissimus, kidney and tailhead fat obtained from each carcass were measured by gas chromatography technology.

日粮中分别添加相当于40.0g粗脂肪的油菜籽、油葵籽、红花籽和亚麻籽，饲喂成年母羊35d后屠宰，用气相色谱技术测定了羊体背最长肌、肾脏脂肪和尾部脂肪的主要脂肪酸组成，结果表明：日粮对肌肉中FA组成的影响较对脂肪组织的大；在所测定的3个部位中，C14：0最为稳定，不受日粮影响；在脂肪组织中，C16：1、C18：1也不受日粮的影响，但肌肉中C18：1含量因添加红花籽显著增大；添加亚麻籽有提高脂肪组织中C18：3的趋势，其余油籽有使各部位C18：3含量下降的趋势，以油葵组下降最为明显；除油菜籽（富含C18：1）外，富含多不饱和脂肪酸的油籽有使C16：0降低的作用。

The values of D10 and D for the total bacteria in boiled dumplings under chill state were 2.04 kGy and 4.00 kGy, the value of D10 and D for E. coli were 1.25 kGy and 2.00 kGy. TBAS were increased significantly and excessed the trade standard by 5 kGy γ-irradiation. However, the TBAS would be decreased with addition of antioxidants. Lipid peroxide,volatile basic nitrogen,"a" value of color and "b" value of color showed a tendency of increase, and moisture content and Aw showed a tendency of decrease, which did not achieve a significant level(p.05) by 1 to 5 kGy doses of γ-irradiation. Total acidity, amino acid nitrogen and free fatty acid showed no significant differences between treated and control samples. Sensory evaluation values were decreased and were all well within the acceptable level by 1 to 5 kGy doses of γ- irradiation.

辐照能有效降低鲜熟水饺细菌总数和大肠菌群数量，鲜熟水饺中细菌总数和大肠菌群的 D10值分别为 2.04 kGy 和 1.25kGy，D 值分别为 4.00 kGy 和 2.00kGy；0～5kGy 辐照鲜熟水饺 TBA 值随辐照剂量增加而增加，5kGy 辐照已使之超过国家行业标准限量要求；辐照鲜熟水饺过氧化值、挥发性盐基氮、色差 a 值、色差 b值存在随辐照剂量增加而增加的趋势、水分含量存在下降趋势，但变化未达到显著水平(p.05)；对酸度、氨基酸态氮、水分活度、游离脂肪酸含量等其他理化指标没有显著影响；较高剂量辐照使产品感官质量降低，却都在可接受范围内； 4kGy 辐照使维生素 B1下降 31.94%，对维生素 E、蛋白、脂肪、氨基酸、不饱和脂肪酸含量无显著影响。

The mentality of managing the people and "special privilege" bring priority and selfish d transposes the relationships between administrative organs and society, makes the administrative regulations show the tendency to value the administrative power and despise the citizen''s rights protection, think highly of management and look down upon service and take substance seriously and underestimate programmer.

同时，行政管理体制和行政运行机制也影响行政规章的制定：权限不清导致制定行政规章难以服从客观性、科学性；权责不统一导致功利主义倾向；命令与服从式的运行机制影响行政规章的合法性与适度性；封闭式的行政运行机制使行政规章脱离实际；部门起草行政规章草案使行政规章框架服从于部门运行机制而丧失科学性；行政机关立法人员素质影响行政规章质量。

The design of the dry-dock falling gate"s structure is researched, and integrates the disquisitions and analysis on the dynamic characteristics about dock gate, in order to make the cabin design more visualizing.(2) The emphasis of this paper is the influence of the water hole and gas hole in the tidal cabin and the influence of the drain of the air-operated cabin.(3) In this paper, the building method of the dry-dock falling gate"s parametric model is studied and the dynamic analysis is accomplished in ADAMS, furthermore, the rising-and-falling angle curve is drawn while the dry dock gate is rising and falling.(4) The paper is based on the apprehension of the past theories and researches about air-operated dry-dock falling gate, and is explicated by hydraulic rationales and rising-and-falling mechanical principles. This software is developed by inner programming language in ADAMS and VC. This design could shorten design cycle.

1研究了气控式干船坞卧倒门的结构设计，整合对卧倒门在海水中运动中的动力特性研究，使卧倒门的设计，特别使其舱格设计更具可视化；(2)重点讨论潮汐舱水孔气孔对卧倒门水上运动的影响，空气操作舱充排水对卧倒门运动的影响；(3)研究了气控式干船坞卧倒门仿真模型的参数化方法和过程，并在ADAMS中完成了系统的动力学分析，绘制出了卧倒门在起卧过程中的运动特性曲线；(4)通过对已有的气控式干船坞卧倒门的实验成果和成形理论的解读，在理解其起浮和卧倒机理基础上，应用VC和ADAMS二次开发语言进行设计软件开发，定制了友好的用户化中文人机交互界面，使设计人员可以方便快捷地对卧倒门门进行设计开发，缩短了设计周期。

Results showed that the activity of soil poly-phenol oxidases and acid phosphatase, and soil respiration were suppressed by Starane at 0.5 and 5 mgkg^(-1) dry soil, respectively, during the first several days of experiment, but were slight promoted thereafter. In contrast, treatment with Starane at 50 mgkg^(-1) stimulated at the beginning of experiment but inhibited following that time the activity of soil poly-phenol oxidases and acid phosphatase and soil respiration. Activity of urease was stimulated at the beginning but inhibited in the following days by Starane at 0.5 and 50 mgkg^(-1) dry soil, respectively. However, the pattern of stimulation of urease activity at first several days of experiment followed by reduction to the basil level was observed. With respect to invertase, its activity was constantly depressed in the presence of Starane.

结果表明使它隆在0.5mgkg^(-1)和5mgkg^(-1)质量分数时对土壤多酚氧化酶、酸性磷酸酶和呼吸强度的影响为轻微抑制－激活－恢复稳定的趋势，50mgkg^(-1)质量分数时为轻微激活－抑制－恢复稳定的趋势；使它隆在0.5mgkg^(-1)和50mgkg^(-1)质量分数时对土壤脲酶活性的影响为激活-恢复稳定的趋势，5mgkg^(-1)时则处于抑制－激活的趋势；各质量分数的使它隆浓度对土壤蔗糖酶和土壤全酚均为抑制作用，且质量分数越大抑制越强烈。

How can the European Union contribute to the development of a European film and television program industry which is competitive in the world market, forwardlooking and capable of radiating the influence of European culture and of creating jobs in Eur ope?

欧洲联盟应该怎样做才能对欧洲的电影电视工业有所贡献，使它在国际市场上具有竞争能力，所贡献，使它在国际市场上具有竞争能力，使它有能力发挥欧洲文化的影响，能力发挥欧洲文化的影响，并且能够在欧洲创造更多的就业机会呢？

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。