作用周期

The expression level of metabolitic enzyme (CYP1A1, CYP1B1) and its correlation with cell differentiation /apoptosis were studied as well. The influence of resveratrol in cell cycle was determined with FCM. Results 1 Resveratrol not only suppresses the growth of medulloblastoma cells in a dose and time related fashion but also induces cell differentiation and apoptosis. Resveratrol promotes differention of UW228-1,-2 cells to forward glia, UW228-3 and Med-3 cells to neuron. A treatment of resveratrol in the concentration of 100μM for 48hrs comit most of cells die of apoptosis. 2 Among the four cell lines Fas and Caspase-3 were constantly expessed, whereas FasL was absent irrespective to the drug treatment. 3 ICC, RT-PCR, Western-blot hybridization and EROD enzymatic activity assay demonstrated that expression of CYP1A1 is different after treatment with resveratrol in four cell lines, up-regulated in three UW228 cell lines in a dose dependent manner but down-regulated in Med-3 cells. Suppressive effect of resveratrol on CYP1B1 expression is the same among four medulloblatoma cell lines. 4 Cell cycle distribution of UW228-3 was greatly changed after treatment.

结果：1、白藜芦醇以时间和剂量依赖性方式抑制髓母细胞瘤细胞生长，同时促进细胞的分化和凋亡：白藜芦醇促使UW228-1、-2靶细胞向神经胶质细胞方向成熟分化，UW228-3、Med-3细胞向神经元细胞方向成熟分化，以100μM作用48小时效果显著；2、四个细胞系均有Fas和Caspase-3表达，但无Fas-L基因表达和蛋白活性产生，因此难以形成Fas相关性死亡通路；3、白藜芦醇处理前后CYP1A1基因的表达在各细胞系略有不同：UW228-1，-2，-3三个细胞系白藜芦醇以剂量相关性方式上调CYP1A1的表达，而Med-3细胞系，白藜芦醇却抑制CYP1A1的表达；四个细胞系在白藜芦醇处理后CYP1B1的表达均受到抑制；4、UW228-3细胞系经白藜芦醇作用后细胞周期有较大改变，表现在G0/G1期在整个细胞周期中所占的比例增加，而其它时期则相应减少。

Some investigations suggested that cyclins and CDK played an important role in the regulation of endomitosis of megakaryocyte. However, the mechanism of cyclins and CDK in the regulation of endomitosis of fetal megakaryocytes during ontogenesis and the relationship between cyclins/CDK and retardation of endomitosis of fetal mgakaryocyte is totally unknown.

一些研究发现周期蛋白和CDK在巨核细胞核内有丝分裂的调控上起重要作用，然而，周期蛋白和 CDK在胎儿巨核细胞核内有丝分裂调控上所起的作用，及其与胎儿巨核细胞核内复制延迟的关系目前全然不知。

The character of motion of electron in periodic structure under magnetic field is analyzed in the article.

周期结构中的电子在磁场作用下的运动摘要：本文对周期结构中的电子在磁场作用下的运动规律做了1些分析。

In our study, we found menadione has anticancer potential on human breast adenocarcinoma MDA MB 231 by weather cell cycle arrest, anti proliferation, or apoptosis. Low concentration of menadione (5.8μM) significant inhibited growth curve on breast cancer cells (MDA MB 231) but not breast cells (MCF 10A) by performing MTT and trypan blue cell counting. RT-PCR and western blotting showed menadione increase p21 gene and protein expressions but decrease cyclin E and CDK2 protein expression resulting in cell cycle G0/G1 arrest by flow cytometry.

MTT及生长曲线的实验，发现menadione在低浓度且长时间作用情况下，对在於人类乳腺上皮细胞癌(MDA-MB 231)是有明显的抑制作用，而在正常乳腺细胞(MCF-10A)则没有影响，在分子转录上及蛋白质表现方面也发现低浓度menadione处理24时能使MDA-MB 231诱发p21基因及蛋白表现量增加及cyclin E、CDK2抑制，再经由流式细胞仪分析也发现低浓度 menadione 处理24小时能使MDA-MB 231细胞周期GO/G1比例上升，造成细胞周期停滞，进而抑制细胞生长。

Proliferation assay, cellular cycle and apoptosis analysis, invasion and migration assay and adherency assay showed that GEBP11 could inhibit the proliferation of Co-HUVECs and HUVECs, induce the apoptosis of ECs, but not alter the cell cycle of ECs.

MTT细胞增殖实验、细胞周期及凋亡分析、细胞侵袭迁移实验、细胞粘附实验显示，GEBP11短肽对Co-HUVECs及HUVECs的增殖表现出不同程度的抑制作用，对Co-HUVECs抑制作用更强，GEBP11能诱导内皮细胞的凋亡，而对细胞周期无明显影响。

3When subjected to the seismic ground motion of the shock type, the shallow, thin and narrow liquefied lenticle may amplify the response of the building with short period.

计算结果表明：(1)所采用的方法与已有的模型实验结果有很好的对应关系，可用于分析建筑物存在下的场地砂土液化问题；(2)饱和砂土透镜体液化对短周期建筑物主要起到隔震作用，且随着透镜体厚度、埋深和宽度的增加以及场地输入地震动加速度的增大，减震效果有所增加；(3)在冲击型地震动作用下，埋深浅、宽度和厚度小的液化透镜体有时却对短周期结构加速度反应起到增大作用。

Reverse-transcription PCR assays were used to detect the mRNA expression level of related regulatory genes such as p15, p16, p21, p27, p57, surviving, cyclin B, cdc2 and chk1, and Western blot assay to detect the level of protein expression and phosphated change such as survivin, cdc2 and chk1. Results:(1) K562 cell arrested on G2/M phase were obviously increased after co-cultured with 2 to 10μmol/L Arsenic trioxide for 24 hours. The ratios of control group, 2μmol/L, 5μmol/L and 10μmol/L groups were 22.6±3.4%, 27.2±2.3%, 43.8±4.5% and 36.7±4.1%, respectively.

体外培养K562细胞，以不同浓度AS_2O_3作用不同时间后采用PI染色、流式细胞仪检测药物作用后细胞的周期分布改变；逆转录酶多聚酶链扩增方法检测细胞周期相关调节基因(p15、p16、p21、p27、p57、survivin、cyclinB、cdc2、chk1)的mRNA表达变化；Western blot方法检测细胞周期相关调节蛋白表达及磷酸化改变(survivin、cdc2、cdc2-p、chk1)。

Objective To establish human oral squamous cell carcinoma cell line ,to ascertain its origin of tissue,and research curcumine's correlated toxicant effect toward squamous cell, to detect a series of bionomics after applying curcumine,such as cell morphous ,growth cycle,the expression of nuclear factor and cyclin .

研究目的旨在建立人类口腔鳞状细胞癌(oral squamous cell carcinoma，0SCC )细胞系，确定其组织来源，并研究姜黄素对鳞癌细胞的相关毒性作用，对作用后的细胞形态、生长周期、核转录因子蛋白及细胞周期蛋白的表达等进行一系列生物学特性检测。

These data suggested that CDC25A might be a promising novel target for cell-cycle-regulating anticancer dugs.

最近研究表明，ART除了具有经典抗疟活性以外，还可通过调控细胞周期对人体多种肿瘤细胞发挥抑瘤作用，但ART是否可通过调控细胞周期而对人食管癌发挥抑瘤作用，其调控细胞周期是否与CDC25A有关尚不十分清楚。

Cyclin-dependent kinases has effects on cell cycle by modulating function of eukaryon organism.The functions have been further studied in cell cycle and the process cell cycle,which will be beneficial to explain tumorigenesis pathomechanism and the new way of oncotherapy.

细胞周期素依赖性激酶是调节真核生物细胞周期的关键分子之一，深入研究细胞周期素依赖性激酶在细胞周期与细胞生长中的作用，对于阐述肿瘤发生、发展的病理机制以及肿瘤治疗的新途径具有重要的意义。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。