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As the DDP group A549DDP cells were treated with IC10 of cisplatin for 48 hours. As the DDP+Rh2 group A549DDP cells were treated with IC10 of cisplatin and IC5 of gensenoside Rh2 for 48 hours. As the controlling group A549DDP cells were not treated with any kinds of drugs. Changes in cellular morphology were observed by fluorescence microscopy. Peak value apoptosis of A549DDP cells were inspected by flow cytometry. State of PTP was evaluated by ultraviolate spectrofluorometer. Changs of calcium in cells and membrane potential of mitochondrion were determined by flow cytometry. Release of cyt-c from mitochondrion with westton blot Radiative activity of〓Tc-MIBI ingested by cells was measured withγcounter. Results: IC50 of cisplatine to A549 cells was 24uM.
以无毒浓度的Rh2单独作用于A549DDP细胞作为Rh2组,以低效浓度的顺铂单独作用于A549DDP细胞作为DDP组,以无毒浓度的Rh2和低效浓度的顺铂联合作用于A549DDP细胞作为Rh2+DDP组,作用时间为48小时,以不加药物干预作为对照组,Hoechst33258染色荧光显微镜下观察细胞形态的变化,流式细胞仪检测细胞凋亡峰;紫外光分光光度计检测线粒体PTP开放情况,流式细胞仪检测细胞内钙离子浓度及线粒体跨膜电位的变化,Westton blot检测线粒体内细胞色素C释放情况及Caspase-3的活性,γ计数仪检测细胞摄取〓Tc-MIBI放射性活性。
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About the style I study the close intrinsic relationship between his preferring to prose and his philosophy of life and how the changes of his prose style show the ones of his thoughts. Concerning the subject matter of writing I discuss how Zhou" s loving life and "common humanity influenced it. For example, he like to write the fate of unimportant person, the local snacks, the folk arts, the slight changes in nature and so on. As regard to the genre of writing, I set forth that the contradiction of his philosophy of life make his works present the contradictious style which not only is peaceful but also shows his teeth.
关于文体,着重研究周作人的人生哲学和他对散文的偏爱有什么样的内在联系以及他一生不断创造和尝试不同的散文体式背后的思想变化;关于题材,着重论述周作人对生命的关注热爱与&平凡人道观&在其选材上的体现,如喜欢写小人物的命运故事、不起眼的地方小吃和民间手工艺、大自然的细微变化等等;关于风格,着重阐述周作人人生哲学的矛盾性如何使他的散文也呈现出一种&平淡腴润&与&浮躁凌厉&矛盾统一的复杂艺术风貌。
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A total of 16 pairs of digenic interactions were detected including 4 pairs under drought stress with a total contribution of 18.57% and 12 pairs under normal conditions that accounted for 38.49% of the total.
检测到显著互作效应位点16对:其中干旱处理下有4对显著互作,联合贡献率为18.57%;正常供水下有12对显著互作,联合贡献率达38.49%。
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The results showed that tiller number is mainly controlled by both the general genetic factor and the GE interaction factor (with 61.7﹪ of the general heritability and 17.2﹪ of the GE interaction heritability).A total number of 19 QTLs was detected to associate with tiller number. Of these, 9 and 6 QTLs were with single locus effects and digenic effects, respectively. The epistatic effect between QTL 1-8 and QTL 1-12 was suggested to be a major effect due to the contribution of 21.6﹪ in spring season.
结果表明,分蘖数主要由普通遗传因素和互作遗传因素控制(呈现61.7﹪的普通遗传率和17.2﹪的互作遗传率),共有19个QTLs与分蘖数有关,其中9个和6对QTLs分别具有单位点的遗传效应和2位点的互作效应,QTL1-8和QTL 1-12的上位性效应由于在春季的贡献率达21.6﹪,因而认为是一对主效。
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Additive effects were predominant, totally explained 75% of the phenotypic variation and often combined with digenic epistasis. Of 12 main QTLs,9 showed Gaoyou alleles decreasing plant height. Most QTLs with QE effects showed ecologically favourable alleles in diverse regions.
株高受多个QTL影响(12个位点具有加性或兼有环境互作效应,5个位点具有互作效应),以加性效应为主,加性效应总和可解释定位群体表型变异的75%左右,并多兼有上位性效应。12个主效QTL中,9个是&高油&等位基因相对&Sollux&有降低株高的作用,大多数加性×环境互作QTL的有效等位基因具有环境选择特异性。
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A scan of the whole genome for digenic interactions detected considerable amounts ofsignificant epistatic gene actions.Furthermore,many homologous pairwise loci with sameepistatic types observed simultaneously in both years,undoubtedly indicating that realinteractions exist in the population.
以双向方差分析方法对全基因组进行两位点互作显著性检测表明,存在大量的基因上位性作用显著影响产量等性状(P<0.01),而且两年能重复检测到同一显著互作乃至同一互作类型影响各性状。
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In this liquid membrane system,P291〔diphosphoric acid〕and TRPOare used as synergistic mobile carrier,N205 as surfactant,liquid paraffin as film-strengthening agent,sulphonated kerosene as solvent,and (NH4)2CO3 solution as inner phase agent.
用P291〔二磷酸〕、TRPO协同流动载体,N205为表面活性剂,液体石蜡作膜的增强剂,磺化煤油作溶剂,(NH4)2CO3作内相试剂的乳状液膜体系,迁移富集钍。
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And it came to pass in the days of Amraphel king of Shinar, A***och king of Ellasar, Chedorlaomer king of Elam, and Tidal king of nations
14:1 当暗拉非作示拿王,亚略作以拉撒王,基大老玛作以拦王,提达作戈印王的时候
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And it came to pa in the days of Amraphel king of Shinar, Arioch king of Ellasar, Chedorlaomer king of Elam, and Tidal king of natio
14:1 当暗拉非作示拿王,亚略作以拉撒王,基大老玛作以拦王,提达作戈印王的时候
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At this time Amraphel king of Shinar, Arioch king of Ellasar, Kedorlaomer king of Elam and Tidal king of Goiim
当暗拉非作示拿王,亚略作以拉撒王,基大老玛作以拦王,提达作戈印王的时候
- 推荐网络例句
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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.
每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。
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Maybe they'll disappear into a pothole.
也许他们将在壶穴里消失
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But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.
但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。