体质上

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

The incidence of asthma are superior to those without allergic constitution, intrinsichyperergic idiocrasy , family history or smoking in the family members.

自身过敏体质、接触过敏原、家族遗传性疾病史、父母吸烟的患儿哮喘发生率较无以上因素的患儿高(P 。05或0.01)。

Ty with the modern trend of thought ineducation and physical education and is able to to meet the needs of modern society and the development of economy.Also,the principles on which the n...

本文认为，突破口在于必须大胆实现观念上的彻底转变，即以终身体育为主线，拓宽体育教育目标，以使学生终身受益为出发点，立足现实，面向社会，着眼未来，增强学生体质。

Methods230 OSAHS patients proved as OSAHS by PSG were divided into two groups: the retrolingual obstruction group and the nonretrolingual obstruction group. Age, body mass index and the main outcomes of PSG were compared between the two groups.

方法回顾分析230例OSAHS患者的上气道阻塞情况，并根据是否存在舌根平面阻塞将患者分为两组，比较年龄，体质指数（body mass index， BMI）及PSG主要监测指标。

Age, body mass index and the main outcomes of PSG were compared between the two groups. ResultsAll 230 patients had an oropharynx obstruction, of them 120 had a retrolingual obstruction, 105 had a nasal obstruction and 53 had a nasal obstruction and retrolingual obstruction.

方法回顾分析230例OSAHS患者的上气道阻塞情况，并根据是否存在舌根平面阻塞将患者分为两组，比较年龄，体质指数（body mass index， BMI）及PSG主要监测指标。

Fifty rats in the model group underwent ovariectomy, while the other 20 rats in the control group had sham operations. Three months later, 10 rats in the model group and 10 rats in the control group were selected randomly to detect their body weight, uterus weight, BMD of the left femur and lumbar vertebra, biomechanical characteristics of the left femur, levels of serum Ca2 +, Cr, ALP, SOD, MDA and GSH-Px and serumal resistance to OH(superscript -).

4个月龄雌性SD大鼠70只，随机取50只行双侧卵巢切除术，20只行假手术。3个月后随机从手术组和假手术组各取10只大鼠检测体质量、子宫湿重、左侧股骨及腰椎骨密度、生物力学特性和血清生化指标Ca(上标 2+)、肌肝、碱性磷酸酶水平，以确定骨质疏松模型建立成功。

In Taiwan, tourism industries are the most important annual plans of country development. Confronted with the high competitive environment, it is a critical issue to affirm the marketing resources and capabilities inhered in tourist hotels. Based on marketing resources and capabilities, the tourist hotels can enhance the managerial implementation, improve service quality, and create the competitive advantage.

在台湾，观光相关产业已被列为国家发展之年度重点计划；观光旅馆面临高度竞争的环境下，确认企业内部行销资源与能力，强化企业经营体质，提高服务品质，创造竞争优势，实为国际观光旅馆经营管理上不可忽视之课题。

Bam HI partial digestion fragments from Pseudomonas mallei chromosome were ligated into the Bam HI site of the cloning vector pBR 322 and tranferred into E.

用&鸟枪法&分子克隆技术将鼻疽杆菌基因重组到载体质粒pBR 322的BamHⅠ酶切位点上，再转化到大肠杆菌RRⅠ中。

In theory, these added genes might create substances that could cause harmful reactions among sensitized or allergic individuals.

在理论上，这些被加入的基因也许会产生能导致在敏感性或过敏体质的人的有害反应的物质。

In order to scientifically manage exploration data, three-dimensional database composed of rock mass quality indices is established under the geodetic reference frame. Those indices are obtained from adits, drill holes and outcrops.

大型水电工程往往积累有丰富的勘探资料，如何科学地管理这些资料并将其直观、准确地反映在工程地质图件上，即将岩体结构类型及岩体质量空间变化特征以图形展示，是当前研究的重点和难点之一。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。