体质

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Objective To investigate the association of leptin receptor gene A223G polymorphism with BMI and serum lipid levels in hyperpietic population of China.

目的 在原发性高血压人群中，探讨体质指数和血脂水平与瘦素受体基因A223G突变关系。

Lose weight, poor health, partly numb, cold hand and feet, hyperhidrosis, have a pain in the back, easy to get cold, herpes on the mouth, blocked or watery nose, dysphoria, insomnia or hypersomnia, hypodynamia, lose hair, speckle face and allergy.

体重减轻、体虚力单、局部麻木、手脚易冷、掌腋多汗、腰酸背痛；易患感冒、唇起疱疹、鼻塞流涕、烦躁不安、易患失眠或嗜睡；四肢乏力、指甲成像、脱发、面部褐斑及过敏体质

The incidence of asthma are superior to those without allergic constitution, intrinsichyperergic idiocrasy , family history or smoking in the family members.

自身过敏体质、接触过敏原、家族遗传性疾病史、父母吸烟的患儿哮喘发生率较无以上因素的患儿高(P 。05或0.01)。

Ty with the modern trend of thought ineducation and physical education and is able to to meet the needs of modern society and the development of economy.Also,the principles on which the n...

本文认为，突破口在于必须大胆实现观念上的彻底转变，即以终身体育为主线，拓宽体育教育目标，以使学生终身受益为出发点，立足现实，面向社会，着眼未来，增强学生体质。

Objective To investigate the applied anatomic data of the infrarenal abdominal aorta of adult corpse anatomy and imageology.To explore the length and diameter of the infrarenal abdominal aorta and the relation between the body height and the length of the infrarenal abdominal aorta, supplemen the domestic data of abdominal aortic anatomy and provide the evidence to direct the treatment of abdominal aortic disease.

目的通过收集成人尸体标本和螺旋增强CT影像学腹主动脉资料对肾动脉下腹主动脉至双侧股动脉进行应用解剖学研究，了解肾动脉下腹主动脉、双侧髂总动脉、股动脉的长度和直径的解剖学资料，以及肾动脉下腹主动脉至股动脉分叉部长度与身高的关系，为补充腹主动脉体质资料和指导腹主动脉疾病的治疗提供依据。

In the morning,rats in the model and the drug groups were ingested drink spirits,naming "Erguotou"(56°)(0.5ml/100 per day); In the afternoon, drugs were givenamong drug groups respectively, model group was given saline (1ml/100g per day).Meanwhile, The Model and the drug groups were given innutritious diet(40% flour+20% soybean meal+20%hypo-flour+20% botan-flour+a few soybean oil, salt),besides, those rats were prohibited to eat any food except water from 20:00 everynight to the next morning before gavage, the normal group was given saline and anormal diet.

肝纤维化模型组及药物组，上午：予56°白酒&二锅头&灌胃(每日1次，每次0.5ml／100体质量)；下午：药物组予相应药物，模型组予生理盐水灌胃(每日1次，每次1ml／100体质量)，且每晚八点后禁食至第二天上午空腹灌胃后再予饲料；正常对照组予等量的生理盐水做对照。

Distribution and coordination environment of lanthanum in internodal cells of Chara have been determined by inductively coupled plasma-mass spectrometry and X-ray absorption spectroscopy.

利用电感耦合等离子体质谱和X射线吸收谱研究了稀土元素镧在轮藻节间细胞中的分布和配位环境。

Methods Levels of sEng in peripheral blood and intervillous space blood of 22 patients with preeclampsia (preeclampsia group, 12 cases of severe preeclampsia and 10 cases of mild preeclampsia) were detected by ELISA, and the correlation between levels of sEng and blood pressure, 24 h urine protein and fetal birth weight was analysed.

采用ELISA法检测22例子痫前期患者（轻度10例，重度12例）外周血和绒毛间隙血样sEng水平，分析其与患者血压、24 h尿蛋白定量及新生儿体质量的相关性。

Droop lidded 眼皮低垂: 17, But the wild child boldly gets rid of him with a fluid shield and has yielded a building without welds.

但这狂野的小孩勇敢地以一种流体质除去了他，成功地生产了一座无焊点的建筑。

The objective is to subjugate and discourage the people, because that allows the elite to continue to rule unopposed.

其目的是压制和打击人民的积极性，因为这可以让实权派继续统治不会沦为反对派。

GOD,this is the second time you vanquished me!

天啊，这是第二次你打败了我！