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Methods A lamellar flap was produced in the rabbit cornea with a microkeratome. The flap interface in whole corneas and corneal beds were soaked in the rAAV –EGFP solution for 10 min, BSS solution was applied in the control group. The corneas were harvested at day 1, 3, 14, and 21 after application for the structure analysis of cornea by HE staining. Expression of the transferred gene was monitored by fluorescence stereoscope and laser confocal microscope. Results After 3d of infection with rAAV-EGFP ,slight fluorescence was detected in rabbit cornea by fluorescence stereoscope .

微型角膜刀制作兔角膜基质瓣;转染组用含rAAV-EGFP转染液浸泡角膜瓣和角膜基质床10 min,对照组用等量BSS液浸泡角膜瓣和角膜基质床10 min,不同时间点(1、3、7、14、21d)通过荧光体视镜观察角膜出现EGFP荧光的起始时间及分布情况,收集角膜,其中一半用激光共聚焦显微镜观察、照相,另一半固定后行HE染色。

推荐网络例句

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。