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The model mice were then treated by giving GDNF into the striatum. After a seven-day survival period, the model mice were sacrificed to get the segment of substantial nigra fixed, embedded and coronally sectioned continuously. The microsections were processed by immunohistochemistry using anti -TH and anti-CB antibodies to label DA neurons and CB-containing neurons respectively, which were counted under microscope and analyzed statistically.

利用1-甲基-4-苯基1,2,3,6-四氢吡啶(1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine,MPTP)制备成年小鼠PD模型,通过单侧纹状体定位注射GDNF,取中脑黑质节段,做连续冠状石蜡切片,结合免疫组织化学染色方法,观察各组酪氨酸羟化酶(tyrosine hydroxylase,TH)、钙结合蛋白(calbindin D28k,CB)的表达,光镜观察并细胞计数,统计学分析。

Two-dimensional electrophoresis (2-DE) was used to study the variation expression of proteins of cucumber cotyledonary nodes induced with PP333. The characteristics and functions of differentially expressed proteins were analyzed with MALDI-TOF-TOF/MS and Protein Data Bank.

应用双向凝胶电泳技术研究PP333处理后黄瓜离体子叶节培养物差异蛋白的表达,结合质谱分析和蛋白质数据库检索,确定所检出差异蛋白质的性质和功能。

The different explants, hypocotyl, terminal bud, cotyledonary node, seedling were studied to induce bud or callus with different concentrations of 6-BA and to establish a better system for transformation.

利用大豆不同品种的下胚轴、顶芽、子叶节、原位再生等几种外植体进行诱导植株再生的实验,并通过添加不同浓度的6-BA来观察诱导愈伤组织和出芽的情况,为下一步的转化实验建立一个良好的组培平台。

Cotyledonary node explants were prepared from 5-7 day old seedlings, infected and co-cultivated with Agrobacterium tumefaciens. Resistant adventitious buds emerged after 4 weeks on selective medium.

从发芽5-7天的大豆无菌苗切取子叶节外植体,经农杆菌感染和共培养后,在选择培养基上4周左右出现抗性不定芽。

The renal specimen biopsy demonstrated focal necrotizing and crescentic glomerulonephritis.

肾活检可见小血管壁节段性纤维素样坏死,新月体多见。

At present, regeneration system of cotyledons, hypocotyls, and stems, protoplasts and cotyledons codes have been established among Cucurbitaceae. There are several methods of transformation, such as agrobacteria -medidated genetic, particle gun, pollen tube passage, DNA soaking, ovary embryonary sac injection. Transgenic is tending to antivirotic and delayed maturity. Selection marker used often is NPT II.

目前,在葫芦科植物中已建立了子叶、茎尖、胚轴、茎段、原生质体和子叶节等的再生体系;用于遗传转化的方法有农杆菌介导法、基因枪法、花粉管通道法、DNA浸泡法等;用于转化的目的基因多为抗病毒病基因和延迟成熟的基因;NPTII基因为最常用的筛选标记基因。

In order to reveal the differences among the dimorphic cells, basidiospores, arthrospores and mycelia of Tremellafuciformis were subjected to microscopical observation and electrophoresis analysis.

以银耳的担孢子、节孢子和菌丝体为材料,通过显微观察和电泳分析揭示银耳二型态细胞间的差异性。

In order to reveal the differences among the dimorphic cells, basidiospores, arthrospores and mycelia of Tremella fuciformis were subjected to microscopical observation and electrophoresis analysis.

以银耳的担孢子、节孢子和菌丝体为材料,通过显微观察和电泳分析揭示银耳二型态细胞间的差异性。

In Chapter 1, we will briefly introduce Diophantine approximation and metric Diophantine approximation over the real number field and state some results which are important for our work.

在第二章,我们将介绍在正规Laurent级数体上的Diophantine逼近。第一节罗列了一些定义及我们即将使用的符号。

Some explants were collected indirectly from epicormic shoots which sprouted from branches of plus trees raised on wetting sands, and some were from grafts of plus trees when their scions were grafted onto seedlings for 3 months.

取这些获选表型母树之桉穗、或剪取母树的粗枝在湿砂床上培养萌出之簇生芽、或嫁接成功之接穗新长出枝条上的顶芽或带腋芽的节为培植体,进行试管内培养。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?