体细胞杂种

The rale of plants with 42 chromosomes was up to 49.3%. The va riation of chromosome may have a connection with apomixis.

杂种后代的体细胞染色体数目的变化一方面与远缘杂交有关，另一方面可能与无融合生殖具有一定的联系。

The frequency of cells with univalent at MI and with lagging chromosomes and chromosomal bridge at A I in PMC of F1 hybrids of 4 alloplasmic 1BL:/1RS and non-1BL/1RS wheat male sterile lines with Aegilops kotschyi,Ae. variabilis,Ae.ventricosa and Ae.bicornis cytoplasms were systematically investigated,and the relationship between FCU at M I,FCLB at A I and F1 selfed seed set was analyzed.

系统调查了4类异质(粘果、易变、偏凸、二角山羊草细胞质)1BL/lRS、非1BL/1RS小麦雄性不育系与其恢复系杂种F减数分裂中期Ⅰ出现单价体细胞频率，以及后期Ⅰ出现落后染色体和染色体桥细胞频率，并对中、后期染色体变异率与杂种F自交结实率进行了相关分析。

The characteristics of the grafted "Improved Sweet Orange" seedlings showed various segregations, such as "yellow fresh color of Aurantium type","chimaera of red or yellow fresh color of Aurantium type","red fresh color of Aurantium type","chimaera fruit of 1Vlicroacrumen type" and "variant of Microacrumen type", in the vegetative progenies found so far.

一种是把植物的嫁接变异视为&嫁接杂种&；另一种看法则认为除非两个嫁接亲本体细胞的核真正融合在一起，成为染色体数加倍的新个体，才能称为&嫁接杂种&，而这种&嫁接杂种&至今还未见到有可靠的报道。目前所见到的有关嫁接变异的报道，实际上是一些&嫁接嵌合体&，即两个嫁接亲本体细胞的机械嵌合。

The results showed that karyotypic type of Ya01-21was 2n=104＝100m+4sm（4SAT）, Chromosome length ratio of the longest and the shortest 4.25, the average arm-ratio 1.35, belonging to 2C type of chromosome. Molecule identified combine with chromosome studied indicated that Ya01-21 was a progenies of Erianthus arundinaceum. following the law of n+n; ITS identify showed that Ya01-21 is a progenies of Erianthus arundinaceum, the somatic chromosome karyotypic type of Ya01-36 was 2n=132＝130m+2sm, Chromosome length ratio of the longest and the shortest 3.94, the average arm-ratio 1.27, belonging to 2B type of chromosome, combining the advance of characters the law of 2n+n can be resumed.

研究结果表明，崖城01－21的体细胞染色体核型公式为2n＝104＝100m+4sm（4SAT），最长与最短染色体的长度比为4.25，平均臂比1.35，染色体属2C型，分子鉴定结合细胞学研究认为其为斑茅的真实后代，其染色体遗传为n+n；崖城01－36经ITS签定为真实杂种，其体细胞染色体核型公式为2n＝132＝130m+2sm，最长与最短染色体的长度比为3.94，平均臂比1.27，染色体属2B型，结合其性状的进展，推断亲本向其传递染色体可能以2n+n的方式进行。

To study the molecular mechanism of Larix somatic embryogenesis, a differentially expressed cDNA library of Larix somatic embryo in the period of maturation was constructed using suppression subtractive hybridization. The cDNA from the cultures at the stage of somatic embryo maturation of embryogenic cell line Y35 of L. leptolepis ×L. principis-rupprechtii was used as the tester and the cDNA from its subcultured callus was used as the driver. Eight hundreds randomly selected positive clones were sequenced, and 468 UniGenes were obtained finally.

为研究落叶松体细胞胚胎发生的分子机理，文章以日本落叶松×华北落叶松杂种无性系胚性细胞系Y35体细胞胚成熟阶段培养物的cDNA为实验组，继代培养阶段胚性愈伤组织的cDNA为对照组，利用抑制性消减杂交技术(Suppression subtractive hybridization， SSH)构建了体细胞胚成熟阶段的差异表达基因文库。

To study the molecular mechanism of Larix somatic embryogenesis, a differentially expressed cDNA library of Larix somatic embryo in the period of maturation was constructed using suppression subtractive hybridization. The cDNA from the cultures at the stage of somatic embryo maturation of embryogenic cell line Y35 of L. leptolepis L. princi-pis-rupprechtii was used as the tester and the cDNA from its subcultured callus was used as the driver. Eight hundreds ran-domly selected positive clones were sequenced, and 468 UniGenes were obtained finally.

为研究落叶松体细胞胚胎发生的分子机理，文章以日本落叶松华北落叶松杂种无性系胚性细胞系Y35体细胞胚成熟阶段培养物的cDNA为实验组，继代培养阶段胚性愈伤组织的cDNA为对照组，利用抑制性消减杂交技术(Suppression subtractive hybridization， SSH)构建了体细胞胚成熟阶段的差异表达基因文库。

To study the molecular mechanism of Larix somatic embryogenesis, a differentially expressed cDNA library of Larix somatic embryo in the period of maturation was constructed using suppression subtractive hybridization. The cDNA from the cultures at the stage of somatic embryo maturation of embryogenic cell line Y35 of L. leptolepis × L . princi-pis-rupprechtii was used as the tester and the cDNA from its subcultured callus was used as the driver. Eight hundreds ran-domly selected positive clones were sequenced, and 468 UniGenes were obtained finally.

为研究落叶松体细胞胚胎发生的分子机理，文章以日本落叶松×华北落叶松杂种无性系胚性细胞系Y35体细胞胚成熟阶段培养物的cDNA为实验组，继代培养阶段胚性愈伤组织的cDNA为对照组，利用抑制性消减杂交技术(Suppression subtractive hybridization， SSH)构建了体细胞胚成熟阶段的差异表达基因文库。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失