体素培养

Dioscorea parviflora was treated with colchicines at different concentration and time duration in vitro.

在离体培养条件下，比较了不同浓度，不同处理时间的秋水仙素对小花盾叶薯蓣丛生芽的诱变效果。

In the present experiment, the tender stems of Dracaena cambodiana were used as explants to establish a two-step tissue culture and regeneration system. The effects of types and concentrations of plant hormones on the induction of callus were studied. Based on these, the reactions of callus regeneration to different mutation-induced approaches (60Co irradiation, EMS and colchicines), the regeneration ability of the treated callus as well as the characters diversities (including the appearance traits and cytological observations) of the regeneration plants were investigated.

本试验以龙血树幼嫩茎段为材料，通过对龙血树幼嫩茎段两步培养法再生的研究，建立了龙血树幼嫩茎段离体再生体系，证明了愈伤组织的发生和激素种类与浓度的关系；并在此基础上研究了愈伤组织再生对不同人工诱变方法（60Co辐射、EMS、秋水仙素）的反应、被处理愈伤组织再生能力和再生植株性状多样性、以及再生植株不同生理角度的变异性，得到了2株变异株系。

This protocol incorporates treatment of early differentiating embryoid bodies with actiin A to enhance the specification of the endodermal germ layer, followed by adherent culture in serum-free medium and the final application of a commercial, lung-specific medium.

这一个记录包含（a 用活化素A提高早期分化中的胚状体向内胚层的分化（b在无血清培养基中进行黏附培养和应用商业化的肺特异性的培养基。

The leaves, leafstalk, and stems from grapes were used as explants to induce calli and subculture in medium (100 mL B5 medium containing 3 g sucrose and 0.6 g agar) with different plant hormone combinations (6-BA, KT, IBA, 2, 4-D), and then the resveratol content from those calli was determinated by using HPLC.

以葡萄的叶、叶柄和茎分别作为外植体，在不同激素组合（6-卞氨基嘌呤(6-BA)、激动素、吲哚丁酸、2，4-二氯苯氧乙酸(2，4-D)）的培养基（以B5培养基为基本培养基，含30g/L蔗糖和6g/L琼脂）上，进行愈伤组织的诱导和继代培养，并利用高效液相色谱法对不同来源愈伤组织中的白藜芦醇含量进行测定。

The results showed that PYM could dramatically inhibit callus induction and differentiation of explants.

L-1平阳霉素对小菊品种意大利红离体培养的叶片和茎段进行诱变处理。

In this paper, four trade-proteases were chosen to hydrolyze silver carp muscle protein to obtain hydrolysates with powerful inhibitory activity on Angiotensin Ⅰ-Converting Enzyme in Spontaneously Hypertensive Rats serum.

研究了四种蛋白酶水解白鲢鱼肉的工艺条件，并采用离体培养的方法研究其水解产物对大鼠血管紧张素转化酶(AntiotensinI-ConcertingEnzyme，ACE)的抑制活性。

Methods: The lymphocyte,marrow and spermary which are cultivated outside the body of Guenther are used as the experiment material.

以离体培养的外周血淋巴细胞、骨髓和精巢为材料，按常规的秋水仙素-低渗-空气干燥制片法制片和组型分析。

Our experiment is based on Chlamydia trachomatis cell culture to detect the in vitro susceptibilities of C.

为此，我们通过沙眼衣原体细胞培养，从天津市性病研究所就诊的病人泌尿生殖道分离出30株C.t野生菌株，并运用碘液染色计数包涵体的方法检测了这些野生株在体外对3种常用的抗菌药物四环素、阿奇霉素、盐酸莫西沙星的敏感性。

Methods The whole-length X gene was directly cloned to pcDNA3.1 vector. The recombinant vector (pcDNA3.1-X) was transfected into HEPG2 cells after selection with hygromycin, steady clones (HEPG2-X cells) were obtained. The expression of RhoC protein was analysed with immunohistochemical stain.

用定向克隆的方法构建X基因的真核表达载体pcDNA3.1-X，脂质体转染HEPG2细胞；潮霉素选择培养稳定表达X基因的HEPG2-X细胞；免疫组化鉴定HEPG2-X细胞RhoC蛋白表达。

Methods Using the MS medium as the basic medium, the explants shoot apexes and stem segments were cultured to differentiate the regenerations. The autotetraloid with genetic material doubled was achieved by treating shoot tips with colchicine of different concentrations for different long times.

方法以 MS培养基为基本培养基，培养外植体茎尖或茎段获得组培再生植株；用不同体积分数的秋水仙素对无菌苗茎尖进行不同时间的处理，诱导遗传物质加倍的同源四倍体。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。