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Results We obtained a lysogenic bacteriophage MZTP01with clear plaque and 1 mm diameter. Fragment D with 2362bp (Genebank No. AY639599) was obtained after the phage DNA hydrolyzed by HindⅢ/EcoRⅠ. Among fragment D, the pep gene with molecular weight of 47 kDa and length of 1101bp was cloned and expressed. Recombinant M15 (pQE30pep) was built and overexpressed in Escherichia coli with a 47kDa clear band. At the same place a clear band was observed by Western blot. Judging from the time course expression, we could conclude that PEP protein produced at 1 hour after induction and then increased gradually. PEP protein was mainly in the form of inclusion body in the recombinant and slowed the growth speed of host. Homologous comparison of PEP protein from phage MZTP01 with other PEPs from BLAST were that phage MZTP01 PEP protein had 100% homologe with that of Escherichia coli K12, and most of others took the similarity in the range between 37%~84%.

诱导获得的溶原性噬菌体MZTP01斑点清晰,直径约1mm,成斑时间12h;从噬菌体基因组DNA双酶切(HindⅢ/EcoRⅠ)片段中回收长度为2362bp的D片段(Genbank登录号: AY639599),又从D片段中克隆了长度为1101bp、编码367aa、分子量为47kDa的pep基因,表达载体M15(pQE30pep)在大肠杆菌(Escherichia coli, E.coli)中表达获得了47kDa的清晰表达带,在1h 时开始产生蛋白并有逐步上升的趋势; Western blot 也在47kDa处得到一条清晰的条带;可溶性分析表明PEP蛋白在重组菌株中是以不可溶的包含体形式存在的,该蛋白的产生明显地抑制了宿主的生长速度;噬菌体PEP氨基酸序列之间的同源性比较表明,噬菌体MZTP01 PEP蛋白与来自E.coli K12噬菌体的PEP蛋白的同源性程度最大。

With strong economic and technological strength; company owns international top-level body underwear designer - Mr. Peng Xiuhong, he has toured Japan to engage in body underwear design studies for many years, he is the most authoritative body design instructor, combined with domestic Humanistic design style, and reasonable utilization study the human body, mechanics, and engineering, combined with high elastic fabric°¨of the three-dimensional cutting, the human body to achieve a revolutionary breakthrough to enable women to maintain a perfect body no longer subject to take medicine, injections, acupuncture, the pain of chemotherapy, and non-toxic side effects, and on Body Underwear magnet clamp on health care, the five points on the human body positioning magnetotherapy Massage, can prevent breast cancer, cervical cancer, suspended aging, and promote the body metabolism.

具有雄厚的经济和技术实力;公司拥有国际顶级美体内衣设计师--彭修宏先生,他曾赴日本从事美体内衣设计深造多年,他是国内最具权威性的美体设计导师,结合国内人文设计风格,合理利用人体学、力学、和工程学,结合高弹性杜邦莱卡布料的立体性裁剪,使人类美体达到了革命性的突破,使女性保持完美体形不再受吃药、打针、针灸、化疗的痛苦,并无毒副作用,并在美体内衣上钳上保健磁石,对人体的五大穴位进行定位磁疗按摩,可预防乳腺癌,宫颈癌,缓期衰老,促进人体新陈代谢。

Any of several principally arboreal carnivorous mammals of the genus Martes, related to the weasel, mainly inhabiting northern forests, and having a slender body, bushy tail, and soft fur .

貂几种貂属的主要栖居于树上的食肉哺乳动物的统称,与鼬类动物有亲缘关系,主要栖居在北部森林。体形细长,尾部毛浓密,皮毛柔软

In experimental animals, exposure to a common herbicide, rotenone, induces features of Parkinsonism; mechanistically, rotenone-induced destruction of dopaminergic neurons has been attributed to its inhibition of the activity of neuronal mitochondrial complex Ⅰ.

例如,Greenamyre和他的同事在2000年报道了经大鼠颈静脉慢性灌流鱼藤酮复制了帕金森病的主要特征,包括选择性黑质纹状体多巴胺系统变性,Lewy小体样细胞浆包涵体形成和一些行为变化,例如运动减少和僵直等。从发病机制上考虑,鱼藤酮诱导的多巴胺神经通路损伤被认为是由鱼藤酮抑制线粒体复合体Ⅰ活性引起的。

METHODS AND RESULTS: In this study we investigate their relative roles in mediating aggregate formation under physiological shear conditions.

方法和结果:本文研究了在生理剪切力条件下它们在聚集体形成中的作用。

Lithium metaborate melting technology was used to make the quick analysis flow sheets of rock samples by Inductively Coupled Plasma Atoms Emission Spectrometry.

应用偏硼酸锂熔融技术建立岩石样品电感耦合等离子体原子发射光谱法的快速分析流程,并针对含锂硼酸盐熔融物较难溶解的特点,通过熔剂与样品混匀方式实验、熔融体形成龟裂条件实验和浸取液条件实验,研究三者与熔融物浸取时间的关系,从而选择一个适合ICP-AES分析技术的最佳熔融和浸取条件。

The results show that the composite coating is metallurgically bonded to the substrate and the microstructure is fine and uniform. The hardness of the composite coating is up to 400 HV, which is 2.5 times that of the substrate. Under room temperature and oil lubrication condition, the sliding wear tests indicate the friction coefficient and weight loss of the composite coating are smaller than those of substrate. The worn surface of the composite coatings is much smoother than that of the substrate, without grooves and crater.

结果表明,复合材料涂层与基体形成良好的冶金结合,微观组织细小、均匀;复合材料涂层硬度达400HV,约为基体的2.5倍;在室温油润滑条件下,滑动磨损试验表明复合材料涂层摩擦系数和失重均小于基体,磨损表面划痕较浅且平滑,无明显的犁沟、粘着、剥落现象;激光表面合金化显著提高了材料的耐磨性。

The results indicated that sclerotia was an important sign and a necessary stage in the formation of the fruit body;light,temperature and humidity were the key factors;"Beiqi"residue can stimulate the forming of the sclerotia and the fruit body;the most suitable culture areas for Morchella were poplar forest land and apple orchard;and woodash and poplar-root soil can promote the forming of the fruit body.

结果表明,菌核是羊肚菌子实体产生的重要标志和重要阶段;光照、温度和湿度是子实体形成的关键因子;北芪渣对菌核和子实体的形成有刺激作用;人工栽培适宜在杨树林地和苹果园进行;草木灰和杨树根土可促进羊肚菌子实体的产生。

Syntheses,Crystal Structures and Properties of Multinuclear Ferric Complexes with Organic Carboxylates and N, N-chelate ligand

论文标题:含铁有机酸根和N,N-双齿配体形成的多中心配合物的合成、晶体结构及性质研究

Now, I mainly engaged in myxobacterial genomic and bioinformatics analysis work. Myxobacteria is a single-cell microorganisms, but they act as multi-cell behavior (such as feeding, social movement, cells congregating and fruiting body formation, and so on) and own a wealth of secondary metabolites Product, those remarkable characteristics make myxobacteria important in the research fields of prokaryotes cell differentiation and development, biological evolution, as well as the drug development and research.

现主要从事粘球菌基因组与生物信息学分析方面的研究工作:粘细菌是单细胞微生物,然而却具有多细胞行为(如摄食、社会性运动、细胞聚集和子实体形成等)和丰富的次级代谢产物,这些行为特征使粘细菌在原核生物的细胞分化发育、生物进化以及药物开发研究中占有重要地位。

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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。