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The caprine IL-2 gene and the IFN-γgene without the signal peptidesequence were subcloned into (5") Bam H I and (3") HindⅢrestriction sites of pET30aplasmid, the recombinant plasmid were transformed into E. coli BL21(DE3) and induced withIPTG, they were demonstrated by SDS-PAGE that the genes were expressed as inclusion bodies.

应用DNA重组技术,将IL-2基因和去除信号肽的IFN-γ基因分别插入到原核表达载体pET-30a的BamHⅠ和HindⅢ位点上,构建原核表达质粒pET30a-CapIL-2和pET30a-CapIFN-γ,转化大肠杆菌BL21(DE3),经IPTG诱导后进行SDS-PAGE电泳,结果表明目的基因在大肠杆菌中以包涵体形式融合表达。

The typical Labrador possesses style and quality without over refinement,and substance without lumber or cloddiness.

但这种犬一般不表现出过低和过长的体形。结构:骨骼与整个犬体都是相称的。

Physical structure on lines of strength and activity, free from cloddiness and with no trace of coarseness.

特征体形强壮,活泼积极,外观不能笨重粗鲁。

Physical structure on lines of strength and activity, free from cloddiness and with no trace of coarseness.

体形强壮,活泼积极,外观不能笨重粗鲁。

Physical structure on lines of strength and activity, free from cloddiness and with no trace of coarsenes*.

体形强壮,活泼积极,外观不能笨重粗鲁。

"Either the Atlantic or the Pacific subspecies of Clupea harengus (once considered two separate species), slab-sided, northern fishes that are small-headed and streamlined, with silvery iridescent sides and a deep-blue, metallic-hued back."

鲱形目鲱科体形侧扁的北方鱼类,既指大西洋鲱,也指太平洋鲱(C.harengus pallasii),两者曾被认为是两个种,现则仅作为两个亚种。鲱鱼头小、体呈流线形,体侧闪亮银色,背部深蓝金属色。

"Either the Atlantic or the Pacific subspecies of Clupea harengus (once considered two separate species), slab-sided, northern fish es that are small-headed and streamlined, with silvery iridescent sides and a deep-blue, metallic-hued back."

鲱形目鲱科体形侧扁的北方鱼类,既指大西洋鲱,也指太平洋鲱(C.harengus pallasii),两者曾被认为是两个种,现则仅作为两个亚种。鲱鱼头小、体呈流线形,体侧闪亮银色,背部深蓝金属色。

Objective:To study the Clinical and pathological features of IgA nephropathywith partial crescentic formation.

目的:探讨原发性IgA肾病伴部分性新月体形成的临床和病理特点。

Methods:Clinical and pathological characteristics of 79 patients with IgA nephropathy accompanied by partial crescentic formation were reviewed.

选取79例经肾活检确诊伴部分性新月体形成IgA肾病患者,分析其临床和病理特点,并根据新月体形成所累及肾小球的比例分组:≥10%为A组,31例;≤10%为B组,48例。

Objective: To analyse clinical appearance, therapy and prognosis of idiopathic IgA nephropathy with crescentic formation and capillary tuft necrosis, to find the clinical characters and discuss the therapy.

目的:通过对IgA肾病患者中伴有新月体形成及毛细血管袢坏死者进行临床表现、治疗及预后指标的分析,了解此类患者的临床特点、探讨治疗方案。

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歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车,和一双合并为一移交&整个lotta爱&。

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