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The results showed that there were two kinds of grains in the cells,one was large and had low electron density,Gl for short,while the other was small and had high electron density,Gh for short.The latter ones had a circle around it which can be seen through the microscope.During the aroma releasing period,the shape of the two grains changed distinctly,which led to the inference that Gl was the leucoplast,the store-room of aroma precursors or energe substances,and that the Gh was probably the lysosome which is related to the enzyme system?s aroma producing.The circle around Gh is the formed fragrant constituents and the aroma forming is the result of the interaction of Gh and Gl .

结果发现,茉莉花瓣细胞中存在电子密度较低、体积较大的颗粒和电子密度较高、体积较小的颗粒(分别简称为Gl和Gh颗粒),并在Gh颗粒周围存在清晰的晕圈;在茉莉花释香过程中,两种颗粒在形态上均发生明显的变化,推测Gl颗粒可能是白色体,是茉莉花香气前体物或能量物质的贮存器,Gh颗粒可能为溶酶体,与香气形成的酶系统有关,Gh颗粒周围存在的晕圈可能是香气物质,香气物质的形成是Gl和Gh颗粒相互作用的结果。

It turned out that many inclusions were found in the nucleolus and the cytoplasm of PK15 cell. The paracrystalline arrays was found in the inclusion in cytoplasm whose electron dense is deep; The nuclear of PK15 cell dissolved and there were margination of multiple chromatin aggregates and suspected intranuclear viral inclusion in PK15 cell.

细胞质中包涵体电子密度深,位于核膜周围,内有呈晶格状排列的病毒粒子,直径12±2nm;细胞核内染色质消散,在细胞核内发现多个电子密度深的、圆形或环状、由大量异染色质组成的包涵体,内有细微的粒状物质。

The results show that:(1) after MA for 20h, single phase bcc nanocrystalline supersaturate solid solution formed in Fe84Nb7B9 and Fe80Ti8B12 alloys; the thermal stability of nanocrystalline microstructure in Fe80Ti8B12 alloy was improved significantly than that in Fe84Nb7B9 alloy, and the decomposition temperature of supersaturate solid solution decreased in Fe80Ti8B12 alloy.(2) under 30MPa/900℃/0.5h hot-press sintering conditions, the relative density of Fe84Nb7B9 and Fe80Ti8B12 bulk alloys were 96.7% and 95.5%, respectively. Compared with Fe84Nb7B9 alloy (muti-phase microstructure), Fe80Ti8B12 alloy was composed of superfine crystalline (50~200nm) of single phase α-Fe;(3) the magnetic properties of Fe80Ti8B12 bulk alloy σ(subscript s=198.6emu/g, H=54.6Oe were more excellent than that of Fe84Nb7B9 bulk alloy σ(subscript s=162.9emu/g, H=105.1G, it was related to the different phases formed during hot-press sintering and the grain size of nanocrystalline powder.

结果表明:(1)20hMA后,Fe84Nb7B9和Fe80Ti8B12均形成了单相bcc纳米晶过饱和固溶体,相对前者,后者纳米晶组织热稳定性明显提高,过饱和固溶体相分解温度略有降低;(2)在30MPa/900℃/0.5h热压条件下,Fe84Nb7B9和Fe80Ti8B12块体合金相对密度分别为96.7%和95.5%,相对前者,后者由超细晶(50~200nm)单相α-Fe组成;(3)Fe80Ti8B12块体合金软磁性能σ(下标 s=198.6emu/g,H=54.6Oe明显高於Fe84Nb7B9块体合金σ(下标 s=162.9emu/g,H=105.1Oe,此与纳米晶粉末在热压烧结过程中所形成相的种类和晶粒尺寸大小有关。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体;2)利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达;3)构建ZNRD1的小干扰RNA载体,并测序鉴定;4)利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞(AGS、SGC7901、MKN28)和小鼠成纤维细胞(NIH3T3),G418筛选后进行鉴定;5)利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞(SGC7901)、正常胃组织上皮细胞(GES-1)、对长春新碱耐药的胃癌细胞(SGC7901/VCR)、药敏白血病细胞(HL-60)、对长春新碱耐药的白血病细胞(HL-60/VCR),G418筛选后进行鉴定;6)利用MTT实验检测ZNRD1高/低表达对细胞(AGS、SGC7901、MKN28、NIH3T3、GES-1)生长的影响;7)通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响;8)通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响;9)通过流式细胞仪分析ZNRD1高/低表达对细胞(AGS、MKN28、NIH3T3、GES-1)的细胞周期的影响;10)通过流式细胞仪分析上调ZNRD1对细胞(AGS、MKN28、NIH3T3)的凋亡的影响;11)通过MTT实验检测ZNRD1高/低表达对细胞(SGC7901、SGC7901/VCR、HL-60、HL-60/VCR)体外药物敏感性的影响;12)通过肾包膜下移植法检测ZNRD1高/低表达对细胞(SGC7901、SGC7901/VCR)体内药物敏感性的影响;13)通过流式细胞仪分析ZNRD1高/低表达对细胞(SGC7901、SGC7901/VCR、HL-60、HL-60/VCR)内阿霉素蓄积和泵出的影响;14)通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响;15)通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞(SGC7901、SGC7901/VCR、HL-60)凋亡敏感性的影响;16)通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响;17)利用RT-PCR、Western blot对基因芯片的结果进行鉴定;18)利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用;19)利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用;20)利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响;21)利用反义核酸技术抑制p21的表达;通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响;22)利用反义核酸技术抑制p27的表达;通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响;23)利用脂质体转染法上调Skp2的表达;通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响;24)利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响;25)利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响;26)利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响;27)利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞(SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR)多药耐药表型的影响;利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

The results show that the technical parameters and the concentration of sodium molybdate have obvious effect on the composition of the alloy. The crystal grain size is from 6.2 to 12.7 nm and changes with current density, temperature and the pH value of the electrolyte.The crystalline growth of the alloy accords with the model of three-dimensional growth. The surface morphology is mainly determined by the rate competitions between the growing direction of vertical surface and the expanding direction along the surface towards circumambient. The results of the X-ray data show the presence of the multiphase containing solid solution and intermetallic compound in the deposits. The deposit phases alter from the FeNi and FeMo solid solution to the FeNi and MoNi4structure at 3 A/dm2. The deposits internal stress increases linearly with the decrease of the grain size. The lattice parameter of the deposits are slightly distorted

结果表明:工艺条件和钼酸钠浓度对合金组成的影响较大;所得合金晶粒尺寸为6.2~12.7 nm,并随电流密度、温度和电镀液的pH值的改变而变化;合金的晶体生长符合晶核三维生长模型,镀层表面形貌由平行和垂直于基体的生长速度的相对大小决定;镀层物相为固溶体和金属间化合物的多相结构,当电流密度为3 A/dm2时,镀层物相由FeNi和FeMo固溶相转化为FeNi和

Influence of the magnetic field on the microstructure of AZ91 magnesium alloy;2. The results show that the current density, magnetic field intensity and electromagnetic force in melts are distributed nonuniformly to a great extent.

应用数值方法对直流电弧炉条件下的 Maxwell 方程进行了解析,结果表明,熔体中的电流密度、电磁场强度和电磁力的分布是非常不均匀的,电弧附近的钢水中的电流密度较其它区域高出 1~2 个数量级,电磁力较其它区域高出 2~3 个数量级,该区域的电磁力对熔体搅拌起着决定性的作用,预测了钢水的流形。

PSP is composed of steel wire twined skeleton as the enhanced body and high density PE as the base. The enhanced body and the base are cohered together with a kind of high quality resin.

它以高强度钢丝左右螺旋缠绕成型的网状骨架为增强体,以高密度聚乙烯为基体,并用高性能的粘结树脂层将钢丝网骨架与内外层高密度聚乙烯紧密地连接在一起。

The injection moulding shrinkages of HDPE/LLDPE blend and its blend filled with CaCO3 were studied.

研究了高密度聚乙烯/线性低密度聚乙烯共混物及其碳酸钙(CaCO3)填充体系的注射成型收缩率,分别用示差扫描量热仪和AR2000高级流变仪分析测定了共混物的结晶度和熔体黏度,并探讨了不同配比下共混物结晶度和熔体黏度与成型收缩率的关系。

The injection moulding shrinkages of HDPE/LLDPE blend and its blend filled with CaCO3 were studied. The effects of the crystallization and melt viscosity of the blends with different mass ratio on the shrinkage were analyzed by means of DSC and advanced AR2000 rheometer.

研究了高密度聚乙烯/线性低密度聚乙烯共混物及其碳酸钙(CaCO3)填充体系的注射成型收缩率,分别用示差扫描量热仪和AR2000高级流变仪分析测定了共混物的结晶度和熔体黏度,并探讨了不同配比下共混物结晶度和熔体黏度与成型收缩率的关系。

AbstractObjectives1 Correlate bone mineral densityto vertebral compressive strengths.2 Consummate the technique for percutaneous polymethylmethacrylate vertebroplasty and observe the pattern of PMMA imaging distribution.3 Determine the strength and stiffness of osteoporotic with or without vertebroplasty with PMMA bone cement.4 Correlation of BMD,insertion torque and pull-out strengths of pedicle screws.5 Ascertain whether augmentation with PMMA bone cement can enhance pedicle screw fixation in the osteoporotic spine.6 Ascertain whether augmentation pedicle screw fixation with PMMA bone cement can enhance the stability of unstable thoracolurner burst fractures of osteoporotic spine.

目 的1、测试椎体压缩强度,分析强度与骨矿物质密度(bone mineral density,BMD)的相关关系。2、观察经皮椎体成形术后聚甲基丙烯酸甲脂(polymethylme- thacrylate,PMMA)骨水泥在椎体内的形态学分布及影像学表现。3、比较PMMA骨水泥骨质疏松椎体成形术前/后椎体最大抗压力和压缩刚度的变化。4、分析椎体BMD、螺钉最大旅入力矩和最大拔出力三者之间的关系。5、分析PMMA骨水泥强化骨质疏松椎弓根螺钉后的螺钉的最大轴向拔出力和拔出刚度的变化。6、评价PMMA骨水泥强化骨质疏松椎弓根螺钉脊柱内固定对不稳定型胸腰椎损伤的即刻稳定性和反复载荷后的稳定性。

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