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Some of them undergone sustained division at a density as low as 102/ml or even in a single heterokaryon culture.

培养24—48小时后用稀释法挑选出异核体,部份异核体可在低密度(10~2/ml)甚至单细胞条件下持续分裂。

B3LYP /aug-cc-pvDZ level of theory is applied to study the geometric structures, hyperconjugation and exchange repulsion of 13 isoelectronic equivalents of benzene obtained by successive replacement of CH groups by nitrogen atoms in benzene. More attention is paid to discussing how the energetic properties change upon the number of N atoms.

采用密度泛函理论的B3LYP方法在aug-cc-pvDZ基组上研究了13种苯及其含氮等电子体化合物的分子结构、能量和异构体相对稳定性,重点考察了含氮量对化合物含能性质的影响。

B3LYP/aug-cc-pvDZ level of theory is applied to study the geometric structures, hyperconjugation and exchange repulsion of 13 isoelectronic equivalents of benzene obtained by successive replacement of CH groups by nitrogen atoms in benzene.

采用密度泛函理论的B3LYP方法在aug-cc-pvDZ基组上研究了13种苯及其含氮等电子体化合物的分子结构、能量和异构体相对稳定性,重点考察了含氮量对化合物含能性质的影响。

The sub-structure of bainite in lath is high density dislocation.

10%的残留奥氏体,板条贝氏体的亚结构为高密度位错。

The main results were summarized as follows: 1 With the increase of cooling rate, full recrystallization during cooling became difficult to take place and the decrease in dislocation density became slow so that dislocation density in austenite remains higher before phase transformation occurred. 2 Compared with undeformed samples, the CCT diagram under deform conditions has a similar shape, but its position shifts leftwards.

计算结果表明:1随着冷却速率增大,低碳钢热变形后的冷却过程中再结晶变得越来越困难,位错密度的下降变慢,导致在相变前奥氏体中保留着较大的变形存储能;2奥氏体在经历热变形后的CCT曲线与未变形条件相比,明显向左上方偏移。

The typical apoptotic morphological features appeared in MUTZ1 cells treated with 4 mmol/L VPA for 72 hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dosedependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dosedependent manner, the cells were arrested at G0/G1 phase.

结果显示: VPA对MUTZ1细胞的生长抑制作用呈现时间和剂量依赖性;经4 mmol/L VPA处理MUTZ1细胞72小时后,细胞呈现典型的凋亡形态特征,光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体;透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加,胞浆内大小不规则的染色质团块;流式细胞术结果表明,细胞凋亡率随着VPA浓度的增加而逐步增高,G0/G1期细胞比例随着VPA浓度的增加而逐渐增多,S期细胞比例逐渐减低,细胞被阻滞在G0/G1期。

The typical apoptotic morphological features appeared in MUTZ-1 cells treated with 4mmol/L VPA for 72hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dose-dependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dose-dependent manner, the cells were arrested at G0/G1 phase.

结果显示:VPA对MUTZ-1细胞的生长抑制作用呈现时间和剂量依赖性;经4mmol/LVPA处理MUTZ-1细胞72小时后,细胞呈现典型的凋亡形态特征,光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体;透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加,胞装内大小不规则的染色质团块;流式细胞术结果表明,细胞凋亡率随着VPA浓度的增加而逐步增高,G0/G1期细胞比例随着VPA浓度的增加而逐渐增多,S期细胞比例逐渐减低,细胞被阻滞在G0/G1期。

Re- sults X-ray, CT demonstrated that the centrum natural form vanished differently the ossein density was low, and the garrulous blade swollen around the centrum.

结果 X线、CT显示椎体自然形态不同程度消失,骨质密度低,并见碎骨片向椎体周围膨出。

Methods Proteins existed in adult Schistosoma japonicum membrane were extracted by Triton X-100 and purified through reverse-phase high performance liquid chromatography,and the main protein peaks were then collected separately. 125 I-LDL of human plasma as the ligand, through the radioautography and radioligand binding assay, the protein which can bind human serum 125 I-LDL specifically was identified.

自人工感染家兔的肠系膜下静脉取日本血吸虫成虫,用非离子去污剂TritonX 10 0抽提出日本血吸虫成虫表膜蛋白,反相高效液相色谱进行分离纯化后,收集各主要分离蛋白峰,以12 5I标记的人血浆低密度脂蛋白作为放射性配体,经放射性自显影及放射性受体配体结合分析鉴定能与人血浆12 5I LDL特异结合的蛋白。

The DFT (B3LYP/6-31 + G) method was used to study the relative stabilities and probable proton transfer in isolated and monohydrated 2,4-dithiothymine molecules in gas phase. The effects of water on relative stabilities and the rate of the proton transfer were analysed. The results indicate that only one tautomer for 2,4-dithiothymine can exist in gas phase.

采用密度泛函方法在B3LYP/6-31+G水平上研究了2,4-二硫基胸腺嘧啶孤立分子和水合物的异构体的相对稳定性和可能的质子迁移反应,分析了水分子的参与对 2,4-二硫基胸腺嘧啶异构体的相对稳定性和质子迁移速率的影响。

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随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

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