体大

But the strata with high ground water level are composed of alluvium clays、 silty clay、 gravels and crushed stone soils in Chengdu;And the shield will drive in the interzone with complicated geologic environment, especially in the strata contained more than 50 percent of gravels which diameter is greater than 5cm, also contained big grain size gravels(the maximum diameter is about 1m)、 cement gravels and silty sand with lentoid mass;And on the ground there have many serried buildings in the prosperous zone.

而成都地铁工程将主要穿越由冲积粘性土、粉土、砂砾和碎石类土组成的覆盖层，地下水位高，盾构将通过大量地质条件和环境条件均极为复杂的区间，特别是地层中还夹杂有大漂石、胶结砾石层、粉细砂透镜体，隧道围岩中直径大于5cm的砾石含量一般都在50%以上，最大的漂石直径达1m左右，且地面多为建筑物密集的繁华地区，施工难度大，国内尚无先例，在国外也无完全成熟的经验。

Based on relative literatures,this part has included five resesearch aspects as below.1 Fifteen compounds were isolated and purified by extraction,column chromatography,and their structures were determined on the basis of spectral analysis: acteoside(Ⅰ-1),isoacteoside(Ⅰ-2),crenatoside(Ⅰ-3),cistanoside F(Ⅰ-4),sinapoyl-4-O-β-D-glucoside(Ⅰ-5),adenosine(Ⅰ-6),β-siterol(Ⅰ-7),oleanic acid(Ⅰ-8), succinic acid(Ⅰ-9),caffeic acid(Ⅰ-10),protocatechuic aldehyde(Ⅰ-11),p-hydro xybenzyl alcohol(Ⅰ-12),β-daucosterol(Ⅰ-13),D-galacitol(Ⅰ-14),D-mannitol(Ⅰ-15).Ⅰ-4～15 were obtained from this plant for the first time,andⅠ-6,7,9,and 13 were isolated from Orobanche genus for the first time.2 The scavenging test of DPPH showed that most compounds have comparative antioxidant activity as L-ascorbic acid and part of them show better activity such as the O.coerulescens extract and phenylethanoid glycosides.Acteoside showed potent free radical scavenging effects with a median inhibition concentration of 25.6μg/ml.3 The anti-HBV activities of acteoside,isoacteoside and crenatoside were measured,and all of them showed suppressive activity on the expression of HBsAg and HBeAg in the HepG2.2.15 cell line.

本论文在文献调研基础上对紫花列当化学成分及生物活性进行了研究，并从免疫抗病毒角度探讨紫花列当中特征性成分类叶升麻苷的肝保护作用及其机制。1采用大孔树脂、硅胶和Sephadex LH-20等色谱技术对紫花列当进行系统的植物化学研究，从中分离得到19个化合物，利用UV和NMR等波谱手段及理化性质鉴定了其中的15个化合物，分别为类叶升麻苷(Ⅰ-1)、异类叶升麻苷(Ⅰ-2)、crenatoside(Ⅰ-3)、cistanoside F(Ⅰ-4)、sinapoyl-4-O-β-D-glucoside(Ⅰ-5)、腺苷(Ⅰ-6)、β-谷甾醇(Ⅰ-7)、齐墩果酸(Ⅰ-8)、琥珀酸(Ⅰ-9)、咖啡酸(Ⅰ-10)、原儿茶醛(Ⅰ-11)、对羟甲基苯甲酸(Ⅰ-12)、β-胡萝卜苷(Ⅰ-13)、D-半乳糖醇(Ⅰ-14)和甘露醇(Ⅰ-15除化合物Ⅰ-1、Ⅰ-2和Ⅰ-3外，其余化合物均为首次从该植物中分离得到。2)通过大孔树脂富集该药材有效部位苯乙醇总苷，并采用HPLC测得其所含特征性成分类叶升麻苷的含量可达80%以上。3DPPH自由基清除试验显示紫花列当提取物及其所含的苯乙醇苷类化合物均具有较好的抗氧化能力，其清除DPPH自由基能力接近于抗坏血酸。4采用卡介苗整体致敏、脂多糖离体攻击构建大鼠原代肝细胞免疫性损伤模型，在体外观察类叶升麻苷的保肝作用。

Methods Rat bone mesenchymal stem cells were isolated from adult rats bone marrow by Ficoll -Paque density gradients, cultured and amplified in vitro, and then identified by flow cytometry ; Middle cerebral artery occlusion model of regional cerebral ischemia in rats were made by thread embolizing.

方法Fieoll-Paque分离液梯度离心分离大鼠骨髓间质干细胞（rat bone mesenchymal stem cells，rMSCs），经体外培养扩增并流式细胞术鉴定；线拴法制作大鼠大脑中动脉闭塞脑局灶缺血模型，Hoechst33342标记细胞，分别通过静脉移植及立体定向局部移植到大鼠缺血侧纹状体。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。收获的NASC传代培养，用流式细胞仪检测细胞的免疫表型。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum. The acquired NASCs were subcultured for passage.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。

Thus Satan talking to his neerest Mate With Head up-lift above the wave, and Eyes That sparkling blaz'd, his other Parts besides Prone on the Flood, extended long and large [ 195 ] Lay floating many a rood, in bulk as huge As whom the Fables name of monstrous size, Titanian, or Earth-born, that warr'd on Jove, Briareos or Typhon, whom the Den By ancient Tarsus held, or that Sea-beast [ 200 ] Leviathan, which God of all his works Created hugest that swim th' Ocean stream: Him haply slumbring on the Norway foam The Pilot of some small night-founder'd Skiff, Deeming some Island, oft, as Sea-men tell, [ 205 ] With fixed Anchor in his skaly rind Moors by his side under the Lee, while Night Invests the Sea, and wished Morn delayes: So stretcht out huge in length the Arch-fiend lay Chain'd on the burning Lake, nor ever thence [ 210 ] Had ris'n or heav'd his head, but that the will And high permission of all-ruling Heaven Left him at large to his own dark designs, That with reiterated crimes he might Heap on himself damnation, while he sought [ 215 ] Evil to others, and enrag'd might see How all his malice serv'd but to bring forth Infinite goodness, grace and mercy shewn On Man by him seduc't, but on himself Treble confusion, wrath and vengeance pour'd.

V2：撒但这样对他最亲近的伙伴说着，把他的头抬出火焰的波浪上面，两只眼睛，发射着炯炯的光芒，身体的其他部分平伏在火的洪流上，又长又大的肢体，平浮几十丈，体积之大，正象神话中的怪物，象那跟育芙作战的巨人泰坦，地母之子，或象百手巨人布赖利奥斯，或是古代那把守塔苏斯岩洞的百头神台芬，或者象那海兽列未坦，就是上帝所创造的一切能在大海洪波里游泳的生物中最巨大的怪物：据舟子们说，他有时在汹涌的挪威海面上打瞌睡，常有小舟夜航而遇险的时候，以为他是个岛屿，抛锚扎在他的鳞皮上，碇泊在他身旁的背风处，在黑夜的笼罩中等待姗姗来迟的黎明。大魔王就是这样横陈巨体，被锁在炎炎的火湖上面，既不能起立，也不能昂起头来，但由于那统治万汇的天神的意志和他的洪量，让他自由地得逞阴谋，他心想危害别人，却终于加重自己的罪行，刑上加刑，让他懊恼地看见自己一切的恶意怎样在他所引诱的人身上带来无穷的善意、恩惠和怜悯，而在他自己身上却招来了三倍的慌乱、惩罚和报复。

Our results shown: Compared with those of the unradiated rats, the number of S-100 protein immunoreactive positive glial cells in the brains of the irradiated rats increased gradually. More S-100-positive glial cells with multiple long processes and hypertrophic cell bodies were also observed in these areas.

在此基础上，又做了同一照射剂量（20Gy）照射大鼠半脑后不同存活期间（id，7d；14d，Zld，28d）大鼠全脑S－100动态变化的观察，发现其规律如下：在上述时间点大鼠脑内各部位卜刁m蛋白免疫反应细胞数量进行性增加，伴有 S－100免疫阳性细胞的胞体逐渐增大和交起增多，与正常对照组具有明显的差异。

About feeding thick material flush ioint machine,Usee five connecting rods to be possible to adjust about thickness the compound feeding,to swollens the wseam material and dives moves the natural big especially thick material and the thin material,Can enable the lower level in the tailoring process the feeding quantity to achieve consistently;Uses turns on lathe the shuttle greatly,The raduction trades the agent number of times,Most major characteristic thickness dual purpose,Enhances the working efficiency,Uses the completely automatic lubricating arrangement,Enhances the machine service life.

0388系列上下送厚料平缝机使用五连杆可调整厚薄上下复合送料，对膨体类缝料和潜移性较大特厚料和溥料，在缝纫过程中能使上下层的送料量达到一致；采用大旋梭，减少换底线次数，最大特点针线张力小；缝纫厚度能力增大，缝纫薄料性能优良。

METHODS: Differences of the vasodilating action of Ipt in big artery and small artery were studied using isolated rat tai l artery helical strips and aortic rings, and the effects of Ipt on potassium cu rrents in smooth muscle cells derived from rat tail artery were observed by usin g patch clamp technique.

采用大鼠尾动脉螺旋状血管条和主动脉离体血管环两种组织，对比观察盐酸埃他卡林对大、小动脉扩张作用的药理学特性，并且利用膜片钳技术观察盐酸埃他卡林对大鼠尾动脉平滑肌细胞钾电流的影响。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。