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Objective To establish human brain-derived neurotrophic factor genetical modified marrow stromal cells and to evaluate the influence on the viability of the cultured spiral ganglion cells from Kunming mice.

目的 探讨人脑源性神经营养因子(brain-derived neurotrophic factor, BDNF)基因工程细胞的建立及其对体外培养的小鼠螺旋神经元生长活性的影响。

To want to make up this kind of congenital blemish viable way can carry on directly transporting nourishment, namely, impassability over the function conversion of digestive system, impassability lead the blood deliver, but directly make the organization of an outside dew absorbed nourishment, this is outside called a body and transport nourishment on the medical science, good news BE, can carry on the product that this kind of nourishment outside the body transports have already developed success, gum's atrophying is not"incurable sickness" any more and want ~only tooth slot the bone have no quilt complete absorb, all theoretically can pass a gum rebirth, afresh firm loose dynamic tooth, use effect and widespreadly respond good.

要想弥补这种先天性缺陷,可行的办法只能是进行直接的输送营养,即,不通过消化系统的功能转换,不通过血液传输,而是直接让一个外露的组织吸收营养,这在医学上被称为体外输送营养,好消息是,能够进行这种体外营养输送的产品已经研发成功,牙龈萎缩不再是"不治之症",只要牙槽骨没有被完全吸收,理论上都可以通过牙龈再生,重新牢固松动的牙齿,使用效果普遍反应良好。

In conclusion, Schwann cells from wallerian degenerating facial nerves of rats might bear with similar biological characteristics and functional phenotype to those from sciatic nerves in vitro, and they might be able to produce soluble NTFs for trophic support on facial motoneuron cultures. The establishment of methods for obtaining high-purity facial motoneuron cultures of rats with partially labeled fluorescence should be of significance in contribution to further investigation on facial motoneuron's response to NTFs and other affecting factors.

综上所述,本文通过面神经雪旺氏细胞体外培养条件下生物学特性、功能表型,及其对培养面神经运动神经元细胞的营养活性作用的研究,为在体外条件下研究雪旺氏细胞对面神经运动神经元的营养支持作用时,如何选择细胞来源,提供了实验依据;建立了获得较高纯度并带荧光标记的面神经运动神经元的分离和体外培养方法,为进一步研究影响面神经生长、损伤后再生修复的因素奠定了实验基础。

To determine the role of recombinant human ciliary neurotrophic factor in myogenesis, we observed the effects of rhCNTF (0-10 ng/ml) on myoblast differentiation of adult human in vitro.

为探讨外源性重组人睫状神经营养因子在成肌细胞分化中的作用,实验观察了0-10 ng/ml rhCNTF 对成人成肌细胞体外分化的影响。

According to the mechanism of block of development in vitro culture on early embryo of mammal and in vivo surroundings of early embryo, the paper states that requirement and utilization of nutrients during each cell stage of early embryo of mammal in vitro culture in order to search for in vitro culture condition and method to improve the development rate of blastosphere.

摘要从哺乳动物早期胚胎体外培养发育阻断机理和早期胚胎的体内环境入手,阐述了胚胎体外培养各细胞阶段胚胎对营养物质的需求,寻求合理的体外培养条件和方法,以便提高体外胚胎早期的囊胚发育率。

B vitamins can demonstrate certain degree of neurotropic and neuroprotective effects on cultured retinal neurons and RGCs.

B族维生素对体外培养的大鼠视网膜神经元和RGCs有不同程度的神经保护和营养作用。

Objective To gain stable genetic modification of neural stem cells that constitutively secrete brain-derived neurotropic factor and to explore the biological role on the survival and neurite outgrowth of cultured dorsal root ganglion neurons.

目的 建立稳定表达脑源性神经营养因子基因的神经干细胞并观察其对于体外培养的脊髓背根神经节细胞存活及突起生长的影响。

The results of the determinating the activity of the CA in vitro showed that the CA of the water—fed individuals were in activing statics after eclosion. Because of the apperence of the Vg in haemolymph and the deposition of the vitellin in oocytes in the unfed females, it was shown that the CA in such individuals were in activiting statics, too. Again because in the water—fed females which did not lack the feeding action the activity of the CA showed dropping tendency gradually still, it could be said that the activiting of the CA was possibly effected by the nutrition but it was never related to the adult feeding action. In another respect, the brain of the female adult had not significant effect on the activiting CA. The oocytes could depositing vitellin in individuals removed brain tissue on the day of eclosion, which showed that the activting of CA was not concerned with brain.

CA活性的体外测定说明饲水个体的CA同样具有活性,不取食个体血淋巴中Vg的存在以及卵母细胞中卵黄的沉积表明,在不取食的个体中CA同样具有活性,所以CA的活化与成虫期的取食动作无关,与成虫是否取食花蜜也无太多的联系,但是CA活性的维持可能受成虫期营养的影响,如果以卵母细胞发育和CA体积大小作为CA活性的指标,则饲水个体CA的活性在后期受到了影响,说明饲水不能替代取食蜂蜜来维持成虫期CA的活性;成虫期脑对CA的活化无明显作用,羽化当日摘除脑的个体,其卵母细胞仍然可以沉积卵黄,说明CA的活化与脑无关,而是受某种不确定的因子的影响;用抗JH物质—早熟素—Ⅱ以10ug/虫的剂量在颈部点滴处理雌虫,对Vg的发生和卵巢的发育具有一定的迟缓作用,其原因有待于进一步的研究。

To want to make up this kind of congenital blemish viable way can carry on directly transporting nourishment, namely, impassability over the function conversion of digestive system, impassability lead the blood deliver, but directly make the organization of an outside dew absorbed nourishment, this is outside called a body and transport nourishment on the medical science, good news BE, can carry on the product that this kind of nourishment outside the body transports have already developed success, gum's atrophying is not"incurable sickness" any more and want ~only tooth slot the bone have no quilt complete absorb, all theoretically can pass a gum rebirth, afresh firm loose dynamic tooth, use effect and widespreadly respond good.

要想弥补这种先天性缺陷,可行的办法只能是进行直接的输送营养,即,不通过消化系统的功能转换,不通过血液传输,而是直接让一个外露的组织吸收营养,这在医学上被称为体外输送营养,好消息是,能够进行这种体外营养输送的产品已经研发成功,牙龈萎缩不再是&不治之症&,只要牙槽骨没有被完全吸收,理论上都可以通过牙龈再生,重新牢固松动的牙齿,使用效果普遍反应良好。

These results implied that processes catalyzed by these proteins probably occurred outside of cells, at least partially.

这些蛋白所参与的生化过程可能发生在膜外的周质空间或是菌体外的场所,为该物种营养的摄取,以及对环境做出响应服务。

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但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊!不用提了。提到肉,真是糟透了。

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Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。