体外发育的

In the present study we transfected F-GFP into cultured hippocampal neurons at 5 days in vitro(DIV 5).Then ASICs antagonists were applied to the neuronal medium to inhibit the function of ASICs.Dendritic growth and arborization of cultured hippoeampal neuron were observed in DIV8 and DIV14. We attempted to determine whether and how ASICs regulate dendritic development.

在本研究中，我们在体外培养第五天(days in vitro，DIV5)的原代海马神经元中转染定位在膜上的GFP，随后加入ASICs拮抗剂抑制ASICs的功能，观察DIV8和DIV14这两个时间点海马神经元的树突生长、分支复杂程度，来研究酸敏感离子通道是否会影响海马神经元的树突发育。

Methods: Selected 93 confirmed cases with hypospadias during May 2003 to Sep 2009. Patients in balanic type were treated with meatal advancement and glandularp lasty; patients with urethra orifice at 2/3 of corpus penis accompanied with slight chordee of penis were treated with Snodgrass method or Onlay method; patients with severe chordee of penis were treated with Duckett method; patients in scrotum or perineum type were treated with combination of Duplay and Duckett; severe hypospadia patients were treated with curved pedicle penis-scrotum and skin flap urethroplasty; patients with good outer plate of ventral prepuce and chordee of penis were treated with ventral pedicle hooded foreskin flap.

选取2003年5月～2008年9月期间收治的93例确诊尿道下裂患者，对阴茎头型患者采用尿道口前移、阴茎头成形法；尿道口在阴茎体外2/3段伴有阴茎轻度下弯者采用尿道板纵切卷管法或加盖岛状皮板法（Snodgrass术或On-lay术）；有严重阴茎下弯的所有阴茎体型采用横裁或纵裁包皮岛状皮瓣尿道成形；阴囊型或会阴型用阴囊中缝皮管加横裁包皮岛状皮瓣成形尿道（Duplay联合Duckett术）；对重度尿道下裂患儿采用弧形带蒂阴茎阴囊联合皮瓣尿道成形术；对腹侧包皮外板发育良好患儿及合并阴茎下曲的患儿采用腹侧带蒂帽状包皮皮瓣法。

Methods: Selected 93 confirmed cases with hypospadias during May 2003 to Sep 2009. Patients in balanic type were treated with meatal advancement and glandularp lasty; patients with urethra orifice at 2/3 of corpus penis accompanied with slight chordee of penis were treated with Snodgrass method or Onlay method; patients with severe chordee of penis were treated with Duckett method; patients in scrotum or perineum type were treated with combination of Duplay and Duckett; severe hypospadia patients were treated with curved pedicle penisscrotum and skin flap urethroplasty; patients with good outer plate of ventral prepuce and chordee of penis were treated with ventral pedicle hooded foreskin flap.

选取2003年5月～2008年9月期间收治的93例确诊尿道下裂患者，对阴茎头型患者采用尿道口前移、阴茎头成形法；尿道口在阴茎体外2/3段伴有阴茎轻度下弯者采用尿道板纵切卷管法或加盖岛状皮板法(Snodgrass术或Onlay术)；有严重阴茎下弯的所有阴茎体型采用横裁或纵裁包皮岛状皮瓣尿道成形；阴囊型或会阴型用阴囊中缝皮管加横裁包皮岛状皮瓣成形尿道(Duplay联合Duckett术)；对重度尿道下裂患儿采用弧形带蒂阴茎阴囊联合皮瓣尿道成形术；对腹侧包皮外板发育良好患儿及合并阴茎下曲的患儿采用腹侧带蒂帽状包皮皮瓣法。

By the method of in situ hybridization, the distribution of mRNA of RSG6 was detected. The result showed that the transcript activity began at the late unicellular stage microspore, increased at the bicellular pollen, and was most vigorous at the two sperm cells in mature pollen.

用地高辛RNA标记试剂盒，采用体外转录方法标记RSG6基因的cDNA序列中长度为258bp的一段作为反义RNA探针，运用RNA原位杂交技术，探讨了RSG6基因的mRNA在水稻根、茎、叶以及花药发育各阶段组织中的分布情况。

And multiple effects of AK auto Ab on ker-atinocyte and possible mechanism of AK auto Ab on recovery of epiderm injury are preliminarily studied.

以往对AK auto Ab在表皮发育、皮肤损伤等过程中的作用大多从动物整体水平进行研究［2，3］，为了进一步明确AK auto Ab在表皮自身稳定中的作用，我们以体外培养皮肤为研究对象，直观地比较不同浓度AK auto Ab对表皮形成的影响，并为阐明AK auto Ab促进皮肤损伤恢复的机制进行初步探讨。

It suggested that a variety of abnormal morphs of G. lamblia trophozoites were observed in vitro axenical cultivation to explor the cause of the phenomenon.

在体外纯培养的贾第虫滋养体中发现一些异常形态的虫体，造成这种现象的原因尚不清楚，其发育过程有待进一步研究。

Follicular oocytes had developed to the stage of GV, GVBD, M I, MII after 0 h, 4 h, 14 h and 24 h of culture, respectively, judged by morphological cretirea of oocyte chromosome stained with orcein.

根据地衣红染色，对山羊卵母细胞的核染色体的形态观察发现，直径为2-3mm的卵泡其卵母细胞在体外分别培养0、4、14和24小时即可发育到GV、GVBD、MⅠ、MⅡ期。

Both the proteins components contained in haemolymph, fat body and ovary and the kenetics of the vitellogenin in haemolymph and ovary were analyzed by SDS—polyacrylamid electrophoresis, Ouchterlony plate tests and Rocket—immuno—electrophoresis. The results showed that the vitellogenesis initiated in adult stage and the Vg in haemolymph was detected firstly at 48h after eclosion. The titer of Vg in haemolymph raised rapidly in following 3 days and almost at the same time the vitellin appeared in oocytes. It was taken about 4—7 days after eclosion for the first eggs to grow up to the matured statics and to be laid out.

SDS-PAGE，双向免疫扩散和RIE分析粘虫生殖周期中血淋巴，脂肪体和卵巢中蛋白质谱带以及Vg滴度的变化，结果表明，Vg发生始于成虫期，Vg在血淋巴中的首现时间在羽化的24小时以后，以后连续三天血淋巴中的Vg滴度迅速上升，与此同时，开始沉积卵黄，卵母细胞逐渐发育长大，到成熟排出体外时大约历时4-7天。

On the contrary, Gin alone can promote the development of intestinal mucosal precursor ceils, but it had little effects on their expansion.

Gln对小肠黏膜前体细胞在体内的发育有显著的促进作用，而对小肠黏膜前体细胞的体外扩增则无明显的作用。

In this test, metabolites and enzyme activities involved in energy metabolism in Cysticercus cellulosae were determined systematicly in vivo and in vitro and under action of drugs.The principles of variation of energy metabolism in Cysticercus cellulosae and mechanism of action of the benzimidazol carbamate drugs were illustrated so as to select an effective drug against immature and mature cysticerci and to provide a theoretical and experimental chemotherapy.

本研究首次系统地测定了体内发育过程中、体外培养条件下及抗囊药物作用下猪囊尾蚴能量代谢的有关物质含量和酶活性的变化，阐明了猪囊尾蚴的能量代谢变化规律和苯并咪唑氨基甲酸酯类药物作用机理，为筛选有效的抗囊药物以及为药物治疗提供了重要的实验和理论依据。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新