体囊

The neural embryo development and yolk sac larval morphological development of E. septemfasciatus was investigated.

本实验对七带石斑鱼胚体、卵黄囊期仔鱼形态发育的过程进行观察，研究其发育特征。

As the male gametophyte, pollen delivers sperm to the embryo sac for fertilization by pollen-tube polarized growth.

花粉作为种子植物的雄配子体，通过萌发后极性生长的花粉管将精细胞送到胚囊完成受精作用。

ARID (AT-rich interaction domain) protein is a transcription factor family in higher eukaryotes that regulates cell proliferation, development, and differentiation. Specificity of DNA binding ability in this family prefers AT-rich sequences, but some ARID family proteins are not sequence-specific DNA-binding proteins or they do not bind AT-rich sequences. We found two genes that encode ARID in Giardia lamblia genome database, garid1 and garid2. We analyzed the function of garid1 first. AU1-tagged gARID1 was found to localize to nuclei. During encystation, gARID1 mRNA level decreased emphatically, but protein level increased. We also found that gARID1 can bind AT-rich initiator of the cwp1 promoter by EMSA. Mutation analysis revealed gARID1 binding sequence was AGATC and AATAAAATA. We used ChIP to demonstrate that gARID1 can bind cwp1 gene promoter in vivo.

ARID(AT-rich interaction domain)蛋白质家族是真核生物的一种转因子，在许多同种的真核生物有它的同源基因，这个家族的蛋白质通常与调控细胞的生长、发育和分化的作用有关，而这个家族的蛋白质和DNA的结合能，各种ARID蛋白质的专一性尽相同，过大致上偏好於和AT-rich的序结合；我们已经在形鞭毛虫的基因组中找到个含有ARID 的基因，分别是garid1和garid2，我们首先对於garid1做分析；将AU1标记接到gARID1转染形鞭毛虫，用免疫萤光染色可发现gARID1存在於细胞核中。gARID1的讯息RNA在囊体化后会明显下，过其阳性染色和蛋白质表现有明显增加；EMSA实验中也发现gARID1会明显的与cwp1基因启动子之AT-rich initiator结合，经由突变序分析，也显示gARID1的结合序为AGATC和AATAAAATA，随后我们也用ChIP证明gARID1在细胞内也的确会和cwp1基因的启动子结合。

A large scope differentiation and a local differentiation occurred during the course of encystment in Tachysoma pellionella and in Euplotes encysticus respectively.

急纤虫形成毛基体吸收型包囊时，细胞结构发生了大范围的脱分化过程。

In blastula embryos and 50% epiboly embryos, the zygotic pou2 transcript is also distributed in every blastomere cell. At the stage of 90% epiboly, expression of pou2 converges to the dorsal midline of embryos and locates in two waves of lateral neural plate and two transverse stripes in the brain region. In embryos at 100% epiboly, pou2 transcript is restricted in the neural plate and areas of presumptive midbrain amid himdbrain. From somite stage, gibel carp pou2 can not be detected any more, which is consistent with the RT-PCR result.

在高囊胚期和50%下包期，高度表达的合子转录本仍在所有的胚盘细胞中，但至90%下包期时，pou2的表达向胚胎背部的正中线汇聚，集中在神经板的两侧区域和脑部的两条横向条带在100%下包期时，pou2的表达集中在神经板的中间区域以及预期形成的中后脑区域至体节期时，转录本消失，这与RT-PCR结果高度一致。

Temporal and spatial expression pattern of gibel carp pou2 during embryogenesis was investigated by RT-PCR and whole-mount in situ hybridization. RT-PCR result indicates that there is maternal transcript of gibel carp pou2 in early embryos and the zygotic pou2 transcript is abundantly expressed from blastula stage. Large amount of pou2 transcript is detected at both 50% and 90% epiboly stages. At 100% epiboly stage, however, the pou2 transcript reduces rapidly and disappears at the later embryogenesis. Whole-mount in situ hybridization shows that the maternal transcript ubiquitously exists in all blastomere cells.

我们用RT-PCR和整体原位杂交的方法研究了银鲫pou2基因在胚胎发育过程中的时空表达图式RT-PCR结果显示，银鲫pou2基因有母源转录本，其合子基因在高囊胚期强烈表达，在50%下包期和90%下包期也有高量的转录本，但在100%下包期表达量急剧降低，至体节期时已经完全检测不到其转录本胚胎整体原位杂交结果显示其母源转录本在所有的胚盘细胞中。

The influences of INA were as follows: The mulcher of pollen parietes was disintegrated or disappeared in certain section and even the entire extine was deleted. The injuries of organelles in the pollen were demonstrated by plastosome peplos imperfect, vesicle sterility; concentric circles of the endoplasmic reticulum or fold arranged, to form the membranal inclusions by enwrapping part of the cytoplasm, and decrease of ribosome amount.

INA细菌对花粉的超微结构的影响表现为：使花粉壁的覆盖层在某些区域解体消失或整个花粉外壁缺失；使花粉中的细胞器受到破坏，包括线粒体被膜不完整，嵴呈囊泡状；内质网呈同心圆卷绕或折叠状排列包围部分细胞质而形成膜内含物；核糖体数量明显减少等。

The embryonic vessels failed to invade into the labyrinthine layer of placenta, which impaired the embryonic-maternal vascular connection. These defects could not be rescued by wild-type tetraploid blastocysts, excluding the possibility that they were due to the extraembryonic tissues.

组织免疫组化分析结果显示：毛细血管腔异常扩张，排列紊乱，不能形成大血管及细致有序的血管网络；胚外中胚层来源的卵黄囊脏层扁平上皮细胞形态变圆，与内皮细胞连接受损；胚胎血管不能侵入胎盘迷路层，胚体与母体血管连接受损。

Of them,194 cases had been conducted sterility explorations,148 ectopic pregnancy operations,91 ovarian multiple cyst operations,49 myomectomy,47 enucleation of ovarian multiple cysts,29 laparoscopic cholecystectomies,24 teratoma resections,2 ruptuure of corpus luteum operations,2 appendectomies,repair in perforation of uterus,eyewinker extraction in abdominal cavity.Tubal ligation and communicating branch lidation of great saphenous vein.

在连续硬膜外加静脉麻醉下为602例行腹腔镜手术，其中不孕症宫腹腔镜探查术194例，宫外孕手术148例，多囊卵巢手术91例，子宫肌瘤切除术49例，卵巢囊肿切除术47例，胆囊切除术29例，畸胎瘤切除术24例，黄体破裂修补术12例，盆腔脓肿引流术2例，阑尾切除术2例，腹腔镜子宫穿孔修补，腹腔异物取出，输卵管结扎，大隐静脉交通支离断等各1例。

Methods:602 patients were performed laparoscopic operation under continuous peridural and intravenous anesthesia.Of them,194 cases had been conducted sterility explorations,148 ectopic pregnancy operations,91 ovarian multiple cyst operations,49 myomectomy,47 enucleation of ovarian multiple cysts,29 laparoscopic cholecystectomies,24 teratoma resections,2 ruptuure of corpus luteum operations,2 appendectomies,repair in perforation of uterus,eyewinker extraction in abdominal cavity.Tubal ligation and communicating branch lidation of great saphenous vein.

在连续硬膜外加静脉麻醉下为602例行腹腔镜手术，其中不孕症宫腹腔镜探查术194例，宫外孕手术148例，多囊卵巢手术91例，子宫肌瘤切除术49例，卵巢囊肿切除术47例，胆囊切除术29例，畸胎瘤切除术24例，黄体破裂修补术12例，盆腔脓肿引流术2例，阑尾切除术2例，腹腔镜子宫穿孔修补，腹腔异物取出，输卵管结扎，大隐静脉交通支离断等各1例。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。