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Total 122 entophytic bacterial strains were categorized preliminarily to 35 groups based on colony morphology. 16S rDNAs were amplified with universal primers of bacteria, all the strains could produce a single band about 1500bp. The PCR products were digested with restriction endoenzyme HaeⅢand Msp Ⅰrespectively, and the different patterns were obtained for each enzyme, by combining all the restriction patterns for the two enzymes, the 122 strains could be grouped into 39 16S rDNA genotypes. The results indicated that endophytic bacteria from oilseed rape were genetically diversity.

分离获得的122株内生细菌,根据菌落形态可以划分为35类;利用细菌通用引物对16S核糖体DNA进行扩增,获得约1.5 kb片段,分别用内切酶HaeⅢ和MspⅠ对扩增产物进行限制性酶切,产生不同的酶切图谱;根据酶切图谱聚类分析结果,所有供试菌株被归为39类,这一结果从遗传上显示油菜内生细菌类群的多样性。

There is a contrary relationship between the growth of fungal hyphae and the accumulation of taxol in the mycelium.

产紫杉醇的内生真菌菌丝体生长和紫杉醇积累之间存在相反的关系。

However, the area is less studied than other areas of endophyteresearch world wide. Therefore, a series of experiments were carried out in laboratory,glasshouse and field to compare the responses of endophyte infected plants andendophyte free plants of perennial ryegrass varieties to 10 fungal pathogensisolated from turf type ryegrass in Lanzhou. The pathogens used in the experimentswere Alternaria alternata、Bipolaris sorokiniana、Fusarium avenaceum、Curvularialunata、Ascochyta leptospora、Fusarium acuminatum、Fusarium chlamydosporum、Fusarium oxysporum、Fusarium solani and Gliocladium roseum. In addition, someprotective enzymes activity and alkaloids contents of E+ and E- plants were measuredand LpsA gene of the common strain - Lp19 and mutant strain -AR1 of Neotyphodiumlolii were sequenced to identify their ...

本研究在初步明确兰州市坪用黑麦草内生真菌及其病原真菌多样性的基础上,在实验室、温室和田间开展了系列研究,比较了带内生真菌和不带内生真菌的黑麦草在离体和整株条件下对细交链孢、离蠕孢、燕麦镰孢、新月弯孢、小孢壳二孢、锐顶镰孢、厚垣镰孢、尖镰孢、腐皮镰孢和粉红粘帚霉等10种病原真菌的抗病性,测定了E+和E-植株数种保护酶活性及其生物碱含量的变化。

Methods:Thyroid function,thyroid microsome antibody,and thyroid globulin antibody were determined with radioimmunoassay in 23 CRF complicated by primary hypothyroidism patients,and the serum calcium,phosphorus,potassium, cholesterol, glucose, urea nitrogen, hemoglobulin,and endogenous creatinine clearance rate were also assayed.

用放射免疫法测定23例CRF合并甲减患者的甲状腺功能、甲状腺微粒体抗体、甲状腺球蛋白抗体及检测血清钙、血清磷、血清钾、血清胆固醇、血清肌酐、血糖、尿素氮、血红蛋白、内生肌酐清除率并与31例单纯CRF患者对照进行分析。

The microstructure of the γ/γ'-α directionally solidified in-situ composites was studied both in as solidified state and as aging treatment.α-fibers, in as solidified state, were arranged paralyzingly in the γ/γ'matrix, and surrounded by the γ' phase.

对内生γ/γ'-α定向共晶复合材料在凝固状态和高温时效状态的微观结构的研究表明,α纤维平行排列在γ/γ'基体中并为γ'相所包围。

These uncontaminated explants showed that morphogenetic potential were 70%-75% and each responding explant had 1.2-1.4 shoots formation on MS medium supplemented with 2 mg/L NAA and 16 mg/L BA. Moreover, adding antibiotics (100 mg/L Rifampicin and 100 mg/L Gentamycin) to solidify MS contained 3% galactose were tested, results showed the lowest contamination rates were 20% and 1.0-1.2 shoots were regenerated per responding explant. The antibiotic treatments with 100 mg/L Rifampicin and 100 mg/L Gentamycin could reduce internal contaminants, while the addition of antibiotics into medium in vitro culture led to phototoxic phenomena of axillary explants, morphogenetic potential lost and the growth of regenerant was also inhibited.

以100 mg/L Rifampicin+100 mg/L Gentamycin或100 mg/L Rifampicin+100 mg/L Gentamycin+150 mg/L Streptomycin组合浓度处理者,具有显著降低污染率的效果分别为60%及50%;克服内生菌污染而建立初代无菌培养之腋芽培植体,则以100 mg/L Rifampicin或以100 mg/L Rifampicin+100 mg/L Gentamycin处理者,芽体再生率最高70%-75%,惟平均每一腋芽培植体芽体增殖数仅1.2-1.4个;另在醣种类及浓度与抗生素试验,显示100 mg/L Rifampicin+100 mg/L Gentamycin之抗生素浓度组合添加至含3g/L半乳糖的培养基,显著降低污染率至20%;但未污染之培植体,形态发生潜能低至40%以内,可反应生长之培植体芽体形成数也仅达1-1.2个之间,再生芽体之生长也显现抑制现象。

Prior research on capital structure by Titman (1988) utilizes structural equation models to find out the determinants of capital structure. In this paper, we use the same methodology and add another endogenous variable – stock return to construct two equations. One regards capital structure and the other one regards stock returns.

Titman (1988)曾利用求解结构方程模式的统计软体–LISREL找寻影响公司资本结构的隐藏因子,本文乃以此篇论文为基础并加以延伸,除了纳入原先的内生变数资本结构外,再加入另一个内生变数–股票报酬率来建立一个双向影响的结构联立方程式,以期研究资本结构与股票报酬率两者是如何交互影响,并是如何由许多因子所共同决定。

The doctoral dissertation focuses on secondary metabolitic material and biological characteristics of two species...

本文对粘帚霉属的两种药用植物内生真菌,进行化学成分和原生质体诱变育种两方面的研究。

Based on the plant growth rate and inhibition zone methods, the antifungal activity of extracts of every organ of Xanthium sibiricum, the fermentation liquid of endophytic fungi MG4-23, and the extract of mycelium were tested with six kinds of pathogenic fungi and three kinds of pathogenic bacteria respectively. The extract of every organ of X.

方法]采用生长速率法和抑菌圈法,分别以水稻纹枯病菌等6种植物病原真菌和大肠杆菌等3种病原细菌为供试菌种,对苍耳各器官提取液、植物内生真菌MG4-23的发酵液及菌丝体提取液进行抑菌活性测定。

The result showed that this strain could colonize in many plants, such as capsicum, tomato, eggplant, cabbage, lettuce, cowpea, wheat, corn and rice.

结果表明:该菌株的内生宿主范围较广,可以通过种子、叶片表面以及根际土壤等进入辣椒、番茄、茄子、白菜、生菜、豇豆、小麦、玉米、水稻等多种植物体内定殖。

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The dynamic variation in content of cndogenous abscisic acid,indoleacetic acid,gibberellinsand isopentenyladenodines of normal juvenilefruit and dropped fruit was measured with enzyme linked immunosorbent assayin the course of physiological fruit drop of 'Shuizhang Longan.

应用酶联免疫法测定了水涨龙眼生理落果期间正常幼果和脱落幼果中内源激素ABA、IAA、GAs及iPAs含量的变化。

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网上数量巨大的代码例程。

MARY, QUEEN OF SCOTS 1542-1587 Oil on panel, 79.190.2 (311/8 × 351/2) By an unknown artist after miniature by Nicholas Hilliard, inscribed, c.

1542年,作为苏格兰国王詹姆斯五世和法国吉斯家族的玛丽之女,玛丽尚在襁褓之中便继承了父亲的王位。