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The lifetime of M intermediate of the three mutants in PVA films were recorded by employing photomicrography. In distilled water, the visible absorption maximum of BRI119T/T121S/A126T and BRI119T/T121S/A126T/E194Q mutants were blue shifted notably by 11 and 12 nm respectively and the BRE194Q's was weakly red shifted in comparison with WT-BR.

与野生型BR相比较,在水溶液中,单突变的可见吸收光谱的最大吸收峰发生了轻微红移,三突变和四突变的最大吸收峰则分别发生了11.0和12.0 nm的明显蓝移。

The lifetime of M intermediate of BR(subscript E194Q) was less than 1.0 s by photomicrography. The lifetime of M intermediates of BR(subscript I119T/T121S/A126T) and BR(subscript I191T/T121S/A126T/E194Q) were 3.0s and 2.0s, respectively.

显微视频录像技术记录的PVA膜中样品的M态寿命表明,野生型BR的M态寿命最短,单突变的M态寿命小于1.0s,三突变的寿命为3.0s,四突变的寿命为2.0s。

The lifetime of M intermediate of the three mutants in PVA films were recorded by employing photomicrography. In distilled water, the visible absorption maximum of BR(subscript I119T/T121S/A126T) and BR(subscript I119T/T121S/A126T/E194Q) mutants were blue shifted notably by 11 and 12 mm respectively and the BR(subscript E194Q)'s was weakly red shifted in comparison with WT-BR.

与野生型BR相比较,在水溶液中,单突变的可见吸收光谱的最大吸收峰发生了轻微红移,三突变和四突变的最大吸收峰则分别发生了11.0和12.0nm的明显蓝移。

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合,而且结合是饱和性的、可被竞争的,符合配-受结合的典型特征,同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性,表明水稻细胞质膜上存在nsLTP的受;利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜,再进行结合实验,结合活性几乎不受影响;而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失,表明其受为没有经过糖基化修饰的蛋白质;利用交联剂BS~3交联配一受后,再进行SDS-PAGE分离和放射自显影,结果显示水稻细胞质膜上的nsLTP受中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合,可能是其受;细胞外碱化实验表明,nsLTP144不能促使拟南芥原生质细胞培养液的细胞外碱化反应,却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质产生的细胞外碱化反应,表明nsLTP和Cryptogein结合细胞膜上相同的位点,保护了植物细胞免受Cryptogein导致的细胞程序性死亡,并诱导系统获得性抗性的产生;外Pull-down实验表明,nsLTP144和水稻的OsCaM1具有相互作用,暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

These structures are conveniently displayed pictorially with links shown as arrows.

每个结构链接到通过成员 next 链接到下一个结构。这些结构就被链接起来。

The effects of different electron donors such as ethyl benzoate diisobutyl phthalate, 2,2,6,6-tetramethyl piperidine and various silanes as internal donor and external donor respectively were investigated.

研究了不同给电子分别作为内给电子和外给电子对MgCl2载催化剂系活性和定向性能的影响。

The predicted sand bodies are distributed in north-cast and approximately north-south orientation. The sandstone reservoir, which is getting thin and pinch out to the high west-north structure, is easy to form the lithological trap with sand body updip pitchout and the structural lithologic trap. Lens sandstones developed and are distributed in source rocks, It is easy to form lithologic traps.

预测砂分布呈现出北东向、近南北向展布特征;向西北构造高部位方向变薄尖灭的储集砂极易形成上倾尖灭岩性圈闭及构造-岩性圈闭,透镜砂岩也较发育,其分布于生油岩区,容易形成岩性圈闭。

Distribution and change of plastid pigment in various phases of tobacco leaves were introduced,and analysis and effect of plastid pigment on tobacco quality were described.

简要介绍烟草不同时期质色素的分布及变化研究现状,综述了烟草质色素的分析方法及对烟草品质影响的研究进展,展望了烟草质色素研究在烟草品控中的应用。

This phenomenon suggests that the interactions between the nucleus and plastids are reciprocal: the development of plastid is controlled by the nuclei, on the other hand, certain nuclear genes by plastids.

这说明细胞核和质之间的作用是相互的:质的发育受细胞核的调控,另一方面,某些核基因的表达也受到质的控制。

It was proved by ultrastructure observations that male plastid excluded during microspore mitosis.

超微结构的研究证明了菜豆雄性质在小孢子分裂时被排除,质全部分布在营养细胞中,初形成的生殖细胞已不含质

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。