体

The phytoplasmas diseases were investigated in 30 counties and cities of 10 areas in Yunnan Province. There are 12 phytoplasma diseases, including paulownia witches-broom,chinaberry witches-broom, cactus witches-broom, christmas cactus witches-broom, orange little leaf, petunia flat stem and so on were indentified using PCR with universal primers, which amplified phytoplasma 16SrRNA gene and/or ribosomal protein genes. So the kind of phytoplasma diseases in Yunnnan Province were understood. Identified the characteristic of 16SrRNA and rp gene of phytoplasma and the kind, classification and genitic relativity of these strains using RFLP, clonging and sequence analysis (nearly 20 full-length sequences of phytoplasma 16SrRNA and/or rp gene were submitted in GenBank ); The phytoplasma strains isolated from herbage plants were conserved in storeroom. 9 articles were published in the key journals of China and 1 technique invention patent was applied. The net-page about phytoplasma disease was established. 4 graduate student were bringed up.

对云南省10个地区的30余个县市地区进行了植原体病害的调查采集工作；采用植原体16SrRNA基因通用引物（R16mF2/R16mR1及R16F2/R16R2）和/或植原体核糖体蛋白基因引物（rpF1/R1）对所采集到的病害标样总DNA进行PCR扩增，根据植原体特异扩增条带的出现，共鉴定出泡桐丛枝、苦楝丛枝、仙人掌丛枝、蟹爪兰丛枝、柑桔小叶、矮牵牛扁茎等12种植原体病害，初步明确了云南省植原体病害的种类；通过RFLP技术、克隆及测序技术获得其16SrRNA及部分核糖体蛋白基因的近全长序列（已在GenBank中共登录近20余个相关序列），通过序列比较分析，明确了上述植原体株系中这两个基因的特征并确定了云南省植原体株系的种类、分类地位及遗传相关性；对一些发生在草本植物上的植原体进行了株系保存；在国内核心期刊及一般期刊发表研究论文 9 篇，申请技术发明专利1项；初步建立了有关植原体病害的网页；培养研究生4名。

We have successfully synthesized hydrocarbonbearing inclusions in carbonate at the temperature and pressure of 150 ℃ and 40 MPa, which are near the condition of natural reservoir underground 4 kmAll synthetic hydrocarbonbearing inclusions have been analyzed by microscope and microfluorescence and fourier transform infrared spectroscopyThe results show that the synthesized hydrocarbonbearing inclusions are very similar to those in real reservoirsOur experiments give us four important insights, including:(1) immiscibility of oil and water is the main reason for hydrocarbonbearing inclusions and aqueous inclusions distributed in different fractures;(2) rapid accumulation of oil and gas can be recorded by fluid inclusions;(3) the study of synthetic hydrocarbonbearing inclusions at the temperature and pressure of real reservoir will offer references and standards for the analysis of fluid inclusions in reservoirs;(4) experiment of synthetic hydrocarbonbearing inclusions is a good method for understanding the mechanism of oilwaterrocks interreaction in reservoirs.

文中报道在实际储层温压条件下(150 ℃，40 MPa，大约4 km深度)成功合成了碳酸盐岩烃类包裹体。通过对合成烃类包裹体的显微观察、荧光分析和傅里叶变换红外光谱分析，证实合成了烃类包裹体，且与真实储层中的包裹体具有相似特征，探讨了油水不混溶条件下的流体包裹体捕获机制。实验研究给出4点重要启示：(1)油水不混溶是造成烃类包裹体和水溶液包裹体分带的主要原因；(2)油气快速成藏过程可以被流体包裹体记录；(3)实际储层温压条件下，合成烃类包裹体实验研究有望为储层包裹体分析提供依据和标准；(4)人工合成烃类包裹体为研究含油气条件下储层水岩作用机理提供了一种有效手段。

The spore germination and gametophyte development of 21 ferns are studied. It indicates that :① the development period of prothallium and young sporophyte of the same species is different due to seeding time of spores;② the optimal temperatures of spores germination and gametophyte development of 21 ferns are about 15 -24 ℃;③ the rate of spore germination of rare ferns is much lower than the ferns which occur large populations in nature;④ the treatment with GA 3 can accelerate the germination of the spores;⑤ the prothallium changes from large to small, green to yellow when young sporophyte comes out. The prothalliums of all 21 ferns die away after the appear of the 3rd leaf of sporophytes;⑥ shapes of the 1st and 2nd leaves of the young sporophyte are different from those emerged later;⑦ spores cultured in dark can not germinate;⑧ a prothallium can develop only one young sporophyte though it has many archegoniums, and the spore propagation with leaf mould substrate is an economical method.

摘 要：以腐叶土为培养基质，对 21 种蕨类植物进行了孢子萌发和原叶体发育的研究，结果表明：①不同时期播种的同种蕨类的孢子，发育出原叶体和幼孢子体所历经的时间长短不同；②孢子萌发和配子体生长发育的适宜温度约为 15 ～ 24 ℃；③稀有蕨类的孢子萌发率低，而在野外能形成较大种群的蕨类的孢子萌发率高；④用 GA 3 处理孢子可以促进萌发；⑤当原叶体上长出幼孢子体时，原叶体由大变小，由绿变黄， 21 种蕨类的原叶体都在幼孢子体上长出第 3 片叶时消失；⑥幼孢子体上长出的第 1 、 2 片叶在形态上与以后长出的叶不同；⑦孢子萌发需要光；⑧ 1 片原叶体尽管有多个颈卵器，但仅发育出 1 株幼孢子体；⑨利用腐叶土进行蕨类孢子繁殖是一种经济实用的繁殖方法。

In this thesis,the AM1,MNDO,MINDO/3(mainly AM1)and INDO/S-CI semiempirical MO methods were used toinvestigate the excited-state intramolecular protontransfer reactions of salicylic acid derivatives—salicylic acid,methyl salicylate,salicylaldehyde,o-hydroxyaceto-phenone,salicylamide and 3-hydroxy-picolinamide (6 conformers and 2-3 anion species);2-(2'-hydroxy-5' methylphenyl) benzotriazole(4 conformers),2-(2' hydroxyphenyl) benzimidazole (3 conformers and 3anion species),Bis-2,5-(2-benzoxazolyl)hydroquinone(3 conformers),2-(2'-hydroxyphenyl)benzothiazole(2conformers) and 7-azaindole dimer (2 conformers).Theinvestigations were described as follows.Geometry optimization,relative stability andhydrogen bonding energy First,for sylicylic acid derivative molecules,the AM1,MNDO and MINDO/3 methods were used toinvestigate ground-state geometry optimization,energies,relative stabilities and hydrogen-bondingenergies on the five kinds of the molecules(designing 6 conformers and 2-3 anion species).Comparing with experimental data,the optimizedgeometry,the order of stability,the hydrogen-bonding energies and the distances between O-O in O-H..O hydrogen bonds by AM1 method were in agreementwith the experimental data,however,the C-C bondlengths optimized by MNDO and MINDO/3 were longer,C-O and O-H bond lengths were shorter;for C-N bondlengths,the results opitimized by MNDO method werethe same as those by AM1 method,nevertheless the C-Nbond lengths given by MINDO/3 method were muchshorter.For some sylicylic acid derivatives(e.g.methyl salicylate,salicylamide),the order ofstabilities on the conformers given by MNDO andMINDO/3 methods were not in agreement with theexisting conformers deduced by experimental methods,and the hydrogen bonding energies calculated by MNDO.and MINDO/3 methods were smaller.Second,the studyon the other systems found that the optimizedgeometry of the proton-transfered product with INDOmethod could not be obtained,only could theoptimized geometry of reactant be obtained,and thecalculated hydrogen bonding energies were greater.Many results of calculation indicated that the studyon the excited-state intramolecular proton transferreaction system using AM1 method was suitable andreliable.

本论文用AM1、MNDO、MINDO/3（主要是AM1）和INDO/S-CI半经验分子轨道方法对水杨酸衍生物系列——水杨酸、水杨酸甲酯、水杨醛、O-羟基乙酰苯酮、水杨酰胺和3-羟基吡啶酰胺（6种异构体和2-3种阴离子）；2-（2'-羟基-5'-甲基苯基）苯并三〓唑（4种异构体）；2-（2'-羟基苯基）苯并咪唑（3种异构体和3种阴离子）；2，5-二间氮杂氧茚氢醌（3种异构体）；2-（2'-羟基苯基）间〓杂硫茚（2种异构体）和7-〓吲哚二体（2种异构体）的激发态分子内质子转移反应在以下几个方面进行了较系统的理论研究：几何构型优化和相对稳定性及氢键能首先以水杨酸衍生物系列分子为例，用AM1、MNDO和MINDO/3方法考察了5种分子（每种分子设计6种异构体和2-3种阴离子）的基态几何构型优化，能量、相对稳定性和氢键能计算，通过和实验数据进行比较，AM1方法给出的优化几何构型、稳定性次序、氢键能和O—H。。。O氢键的0—0距离与实验数据吻合最好，MNDO和MINDO/3方法优化的C-C键长偏长，C-O键和O-H键长偏短；对于C-N键长，MNDO和AM1优化结果差别不大，而MINDO/3给出了过短的C-N键长，MNDO和MINDO/3方法给出的有些水杨酸衍生物分子（如水杨酸甲酯和水杨酰胺）异构体的稳定性次序和实验上推测的可存在异构体结果不一致，MNDO和MINDO/3方法给出的氢键能偏低，对其他体系的研究发现INDO方法常常不能得到质子转移产物的优化几何构型，只能得到反应物的优化构型，并且估算的氢键能偏高，大量的计算结果表明AM1方法对本论文研究的激发态分子内质子转移反应体系是适宜和可靠的。

The results showed that: with the regression analysis of Betula platyphylla, Pinus koraiensis, Abies nephrolepis, Picea koyamai var koraiensis, and Betula costata, there was a significant negative correlation between neighborhood interference index and the growth (0.40~0.89) and the correlation extent depended on the neighborhood influence zone and slope. The neighborhood influence zone corresponding to maximal regression coefficient R~2 was the neighborhood influence radius. Based on the linear subsection regression between neighborhood interference index and influence zone of five species, the neighborhood interference index increased with the increasing zone and there was a previous increasing trend in a certain scope, while the increasing trend slackened beyond the zone. The neighborhood influence zone corresponding to transition point was the neighborhood influence radius. The neighborhood interference index positively correlated with the neighborhood influence zone and the range of R~2 was 0.40-0.64. With the analysis of tree cores of Juglans mandshurica, Fraxinus mandshurica at middle and lower slope in a plot which was fostered in 1995, the distance which the growth release farthest neighborhood and object stub was judged, thus the neighborhood interference radius was inferred. According to the studies, we found that the neighborhood interference radius was different among different trees, even if the neighborhood interference radius of the identical tree species also had the difference at different slopes.

结果表明：通过对白桦、红松、冷杉、红皮云杉和枫桦树种进行邻体干扰指数与生长量回归分析发现，邻体干扰指数与生长量呈显著负相关(0.40～0.89)，相关程度因邻体影响范围和坡位而异，R~2达到最大值所对应的影响范围即为邻体影响半径；通过对白桦、红松、冷杉、红皮云杉和枫桦树种进行邻体干扰指数与影响范围的线性分段拟合，邻体干扰指数随影响范围的增加而增加，其在一定的范围内上升较快，而超出该范围后上升的幅度变慢，变化的转折点所对应的影n向范围可以判定为邻体影响半径，线性回归显著(0.40～0.64)；通过对1995a抚育的胡桃楸、水曲柳样树进行研究，分析邻体树种的中坡位和下坡位的生长锥芯，判断产生生长释放的最远邻木与基株树桩所对应的距离，推断邻体影响半径；根据研究发现，邻体影响半径因树种不同有一定的差异，即使同一树种的邻体影响半径在不同坡位也有一定的差异。

Specifically, itcontains 8 chapters.In chapter 1, the formation, structures, properties and the futureprospect of liposome were thoroughly reviewed;In chapter 2, the stibility and permeability of phopholipid -eleostericacid liposome were studied together with the effect of polymerizationof eleostearic acid. This membrane system was very sensitive to 〓,the effect of 〓 was clarified to increase the aggregation/fusion ofliposomes and made the permeability of mixed liposomes much higher;In chapter 3, two polymerizable conjugated diyne bolaamphiphiles were synthesized. They could form very stable mixed liposome, andthe diyne could be polymerized by UV light in bilayer liposomes, as aresult, the stability of mixed liposome against solvent or surfactantafter polymerization were enhanced. In chapter 4, two kinds of amphiphilic amino acids were synthesized andstable liposomes were formed therefrom After the condensationpolymerization of amino acid in bilayer liposomes, stable polypeptide liposomes were obtained, which had lower phase transition temperatureand higher permeability.In chapter 5, four kinds of glycolipids were synthesized and theiraggregation behavior in water was comparied. When incorporated intophospholipid bilayer membranes, they could increase the phase transitiontemperatures and inhibit the aggregation and fusion of mixedliposomesat lower temperature.In chapter 6 and 7, three kinds of steroidal bolaamphiphiles withdifferent chain lengths were synthesized. Incorporation of steroidalmoiety to the center of lipid bilayer membrane obviously increased themobility of lipid membrane and shifted Tc to lower temperature side incomparasion with cholesterol. The bolaamphiphile which was shorter thanthe hosted lipid bilayer membrane thickness influenced the lipid packingmore obviously.

全文共分8章：第一章对脂质体的形成、结构、性质及展望进行了较为详细的文献综述；第二章研究了磷脂-桐酸脂质体的稳定性，通透能力及桐酸的聚合对这些性质的影响；磷脂-桐酸混合脂质体为一类对〓灵敏的脂质体，〓的作用首先是使脂质体集聚然后使脂质体融合，并加速内包荧光物的释放；第三章通过合成两种可聚合共轭双炔双极性双亲分子DDCA，DDOL，研究了共双炔分子在双分子层脂质体膜上的聚合及对脂质体性质的影响，聚合可以提高脂质体相对于溶剂及表面活性剂的稳定性；第四章合成了两类氨基酸为极性基团的双亲分子，它们均可以在超声下形成稳定的脂质体结构；氨基酸基团可以在脂质体上进行缩聚反应，若聚合后脂质体表面仍有足够的亲水能力，则可得到稳定的多肽型脂质体；聚合后脂质体的相变温度降低，通透能力增加；第五章合成了四种亲水基团为单糖基的双亲分子GL-l，GL-2，GL-3， GL-4，研究了它们在水中的分散情况、集合体形态与分子结构的关系；在DMPC双分子层膜中加入糖脂分子可以使脂质体的相变温度提高，阻止脂质体在低温放置时的集聚与融合；第六章-第七章合成了三种不同碳链长度的双极性含胆甾环双亲分子 CL-1，CL-2，CL-3；它们可以象胆固醇一样与磷脂混合形成稳定脂质体，胆甾环基团位于脂质体双分子层膜的中间；与胆固醇的作用相反，它们可以增加磷脂双分子层膜的流动性，降低混合脂质体的相变温度；三种分子的作用与其碳链长度和磷脂双分子层膜的厚度有关，比膜厚度短的分子影响最大。

Based on the results, it has found that six distinct layers can be identified from lateral to medial in the olfactory bulbs, and the adults have more cells than the subadults. Ventricles of the adults take up larger vacuity, have richer choroids plexus, and primordial pallium and primordial hippocampus of them are more developed than the subadults, in which the primordial piriform is well-developed. Ventral to the primordial hippocampus, there is a septal area which can not be divided into medial and lateral parts in both adults and subadults, nor there is lateral limiting sulcus to separate the primordial piriform and the corpus striatum, but only in the subadults, a medial limiting sulcus can be clearly identified to separate the primordial hippocampus and the septal area. Besides, the corpus striatum of Onychodactylus fischeri is paleostriatum. The cell group that located at two sides of the third ventricle is amygdale, and shape and size of neurons within the telencephalon are poorly differentiation.

结果表明：爪鲵嗅球内细胞从外到内大致可分为6层结构，亚成体的细胞数量少于成体；成体爪鲵的侧脑室所占空间较大，脉络丛更为丰富，原始大脑皮层和原始海马都比亚成体发达，后者大脑内原始梨状区较为发达；隔区位于原始海马的下方，成体和亚成体都没有外侧隔核和内侧隔核之分，也没有外侧界沟作为原始梨状区和纹状体的分界，而在亚成体的侧脑室壁有内侧界沟区分原始海马和隔区，成体则没有；此外，爪鲵的纹状体属于古纹状体，杏仁核是位于第三脑室两侧的两个细胞团，端脑内细胞形态和大小分化较为单一。

Lithologies are mostly sand and carbonate rocks, caps are mud rock, shale and magmatite. Entrap types are anticline, fault block rebuilt by breaking and the screened entrap diapered rock mass. The composition is CO_2, N_2, CH_4, C_2H_6 and He etc. It is magma air source body and its geologic fashions are intrusion and buried volcanic conduit. The relations between gas reservoirs and gas source bodies have three types: magma intrusion-breaking-communicated gas reservoir, magma intrusion-contacted CO_2 reservoir and buried volcanic conduit-contacted gas reservoir. The CO_2 migration in magma intrusion is consisted of fusing and crystallizing phases;it in volcanic conduit is consisted of near-surface effusion and crystallizing phases.The buoyancy of CO_2 in water far more than migration resistance in breaking or chink, CO_2 is easy float upward, the floating can results in differentiation of different density gases and concentration of sealed gas. The gas in sand reservoir firstly migrate into the higher porosity and coefficient of permeability sand, and along with the pressure going up it migrate into the lower. In magma intrusion-breaking-reservoir migration, CO_2 firstly migrate into watered breaking, began gravity differentiation and concentrate, the gas pressure time and again go up, CO_2 migrate into reservoir and concentrate under expansibility as the pressure reach upward a given extend. The CO_2 in reservoir experience four breaking modes: chemistry deposition, dissolution, diffusion and mechanic breaking, the pressure balance can be broken by faulting and the CO_2 will further migrate and form new reservoir.

济阳坳陷已发现的八里泊、阳25、平方王、平南、高青、花17 CO_2气藏主要储集层位有奥陶系、中生界、沙四段、沙三段、沙一段、馆陶组和明化镇组，储集层岩性以砂岩和碳酸盐岩为主，盖层以泥质岩、页岩和岩浆岩为主。；圈闭类型主要为受断裂改造的背斜、断块及刺穿岩体遮挡圈闭。；气体成分主要有CO_2、N_2、CH_4、C_2H_6、He等。；主要气源体为岩浆气源体，气源体的主要地质形式为侵入体和埋藏的火山通道。；气藏和气源体的空间关系有岩浆侵入体一断裂一气藏沟通型、岩浆侵入体-CO_2气储集层接触型和埋藏火山通道-气储集层接触型三种类型。；岩浆侵入体CO_2气运移分为熔融运移阶段和结晶运移阶段，火山通道中CO_2气运移分成近地表喷发阶段和结晶运移阶段。；断裂中，CO_2在水中的浮力远大于运移阻力，CO_2气容易上浮，CO_2在断裂中的易浮性导致不同密度气体的分异和走向上封闭的断裂气体相对富集。；气体在砂岩储集层运移聚集具有选择性，会优先进入孔隙度和渗透率较高的砂岩，随着压力增加，才会进入孔隙度和渗透率较低的砂岩；在岩浆气源体-断裂-储集层空间输导格架下，CO_2气在膨胀力的驱动下，首先进入含水的断裂并重力分异而聚集，气体压力会不断增高，当压力增至一定程度，CO_2气会向高孔隙度、渗透率的储集层运移并聚集。；在岩浆气源体-储集层接触空间输导格架下，CO_2气受膨胀力的驱动直接向储集层运移并聚集。；成藏的CO_2气会经历化学沉淀、溶解、扩散和机械破坏四种破坏方式，会受断裂切割而打破压力平衡，沿断裂进一步运移和聚集成藏。

In meiosis of pollen mother cells of the tetraploids and the hexaploids, only bivalents were observed in most of the populations, although in several populations or individuals a few univalents or tetravalents appeared. In the hexaploids, hexavalents were not observed.

四倍体和六倍体的花粉母细胞减数分裂染色体构型在大多数居群都为二价体(四倍体中30个二价体，六倍体中45个二价体)，少数居群或是个体除二价体为主外还出现单价体和四价体，六倍体类型没有出现六价体构型。

With the development of embryo, Myf5 expression decreased gradually in somites in the anterior region, but remained strong in the newly formed somites; After 30 somites formed, MyoD expression decreased in the somites except the caudal somites. At the hatching stage, MyoD and Myf5 were expressed in head muscle cells and fin muscle cells. In the growing fish, Myf5 was expressed in the skeletal muscle and intestine, and in adult flounder, Myf5 was only expressed in muscle. In the growing fish and adult fish, MyoD was only expressed in muscle.

在胚胎发育早期，Myf5在近轴中胚层中表达，体节发生过程中，Myf5在体节中表达，MyoD基因最早在分节板的体节前细胞中表达，随后在近轴细胞、体节中表达；随着胚胎的发育，Myf5在成熟体节中表达量降低，在新生体节中表达较强；MyoD自30个体节时期后只在新生的尾部体节中表达，在成熟的体节中表达量降低；在孵化期，MyoD和Myf5在头部及鳍的肌肉、尾部的体节中表达；生长期的牙鲆中，Myf5在骨骼肌和肠中表达，成体牙鲆中，Myf5只在肌肉中表达；生长期的牙鲆及成体牙鲆中，MyoD只在肌肉组织中表达。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失