体

Pollen activity of trisomic 2,7,9 was lower than that of diploid significantly;Pollen activity of trisomic 7 was the lowest.

5.9号三体的花粉活力显著低于二倍体；2号、7号三体的花粉活力极显著低于二倍体；7号三体的花粉活力最低。

Some approved aneugens Trip terygiuwlIypogaucum (evelHutch, colchicine, thiabendazole, a tropine sulfate,d-ubocurarine was added into the reactive system respectively and the changes of their absorption values were taken down during different periods under the conditions of 37 0C and 350nm .

本研究通过多步聚合和解聚合反应从猪脑中分离纯化微管蛋白，建立微管蛋白的体外聚合和解聚合反应平衡体系，并将已证实的非整倍体诱发剂（昆明山海棠根部水抽提物、2-（4′-噻唑）苯丙咪唑、硫酸阿托品、d-筒箭毒碱）分别加入此反应平衡体系中，于37℃、350nm条件下，记录此体系不同时期的吸光值变化，与阳性对照物和溶剂对照处理平衡体系后的吸光值在相同时间点作比较，以此评估这几类非整倍体诱发剂对离体条件下的微管蛋白聚合的影响，寻求这几类非整倍体诱发剂是否可通过抑制微管蛋白聚合作用途径而诱发非整倍体。

The invention concerns an artificial block comprising a central core (1) having first (11) and second (12) opposite surfaces each provided with an anvil-shaped side leg (21, 22) with a table (21A, 22A), two sloping sides (21E, 21G; 22E, 22G) and two side surfaces (21B, 21C; 22B, 22C), the tables of said side legs being arranged parallel to said first and second opposite surfaces of the core, the inclined sides of said side legs resting on third (13) and fourth (14) exposed opposite surfaces of said core, said core having fifth (15) and sixth (16) surfaces each provided with side legs and front legs shaped like a truncated pyramid with four side surfaces and one front surface (33) parallel to said fifth and sixth surfaces of the core and to the side surfaces of the side legs.

一种保护层块料，包括一个中心核体(1)，有相对的第一面(11)及第二面(12)，每一面上各有一个砧状侧凸起(21，22)，这两个侧凸起各有一个台面(21A；22A)、两个斜面(21E，21G；22E，22G)和两个侧面(21B，21C；22B，22C)，所述台面相互平行，而且平行于所述中心核体的相对的第一面及第二面，所述侧凸起的斜面支撑于所述核体的相对的可见的第三(13)和第四面(14)。所述核体还有相对的第五(15)和第六面(16)，其上各有一个锥台状的前凸起，该前凸起有四个侧面和一个前表面(33)，该前表面平行于所述核体的第五和第六面，还平行于侧凸起的侧面。

Said simple building comprises building body, roof and pool inside room; the lower building body is complete closed, the upper building body is provided with pillar on lower building body, and is circled with sun-shading net around pillar, the outside of sun-shading net is equipped with movable curtain; the roof is straw roof and has two sides along ridge; the pool inside the room comprises cultivation pool and laying-up basin, each cultivation pool is provide with a overflow bench, the bench surface is equipped with a cushion and the cushion is fixed on slope bench with a plurality of bracing column of same height, and a bamboo curtain is placed on the bracing column for young wood frog rest.

该简易房，包括房体、屋顶和室内池，其下房体为全封闭体；上房体是在下房体上设有房柱，再用遮阳网沿房柱排列的走向围拢，在遮阳网的外面还设有活动帘；屋顶为稻草屋面，沿屋脊坡向两面；室内池包括养殖池和蓄水池，每一个养殖池中均设有漫坡台。坡台面上设有软垫，并有相同高度的多根支撑柱钉穿过软垫固定在坡台上，支撑柱钉上再放置供幼蛙栖身的竹帘。

The zygoma fixture plays a role in transmitting the occlusal force substituting the zagomatic process brace. The deformation tendency of the implants is identical, the displacement of bending is not obviously observed. The displacement of the zygoma fixture near the zygoma is greatly decreased to zero.

结果表明，排骨移植Branemark颧骨种植体种植修复半、全侧上颌缺损模型的应力分布中，应力最大值出现在颧种植体植入颧骨的颈部，颧种植体上应力值较周围支持组织的大，起到了颧突支柱对力的传导作用；种植体结构中弯曲变化的位移不明显，并且种植体整体位移在颧骨附近逐渐减小为零。

Abasic review on dielectric nonlinearity and electric tunable mechanism wasperformed in view of thermodynamics, ferroelectric domain, polar nanoregion,field induced phase transition, grain boundary layer and AC dielectricnonlinearity. The dielectric nonlinearity phenomena in barium stannate titanateceramics were studied and the field dependences of dielectric constant and losstangent were showed.

关于低温介电弛豫行为与极性微区关系的讨论涉及到了弛豫铁电体与扩散相变铁电体之间的相容关系，作者认为所有的扩散相变铁电体都应该是弛豫铁电体或潜在的弛豫铁电体，它们的区别仅在于能够观测到介电弛豫现象的测试频率范围不同。

SEM and TEM observations indicate that the prior austenite deformation not only refines the allotriomorphic ferrite grain but also promotes the nucleation of intragranular ferrite, both of which in turn contribute to the refinement of granular bainite cluster including its ferrite platelets and MA islands.

SEM和TEM观察表明，奥氏体形变不仅细化仿晶界型铁素体，而且促进先共析铁素体在原奥氏体晶内形核，从而有利于细化粒状贝氏体晶团及其内部的铁素体片条和MA岛。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

AVI may be protein matrix and it possesses neither a membrane boundary nor an internal structure,its formation is the result of the anthocyanins transported into the vacuole bind with a protein matrix. In vacuole, AVI is irregular and jelly-like in shape. In AVIs, the attachment of anthocyanins to the matrix protein is likely to be via H-bonds to a sterically restricted site. AVI is suggested to act as vacuolar anthocyanin "trap", preferentially for anthocyanidin 3, 5-diglycosides or acylted anthocyanins. The emergence of AVI can enhance color intensity and results in the "blueness" of color in the vacuole.

花色苷液泡包涵体可能具备蛋白质基质，既无膜包裹又无内部结构，其形成是转运进液泡的花色苷与蛋白质基质结合的结果；液泡里的花色苷液泡包涵体形状不规则，象果冻；在花色苷液泡包涵体中，花色苷可能通过氢键连接于蛋白质基质的一个有限空间位点；花色苷液泡包涵体被认为是液泡中花色苷的&陷阱&，优先摄取花色素3，5-二糖苷或酰化的花色苷；花色苷液泡包涵体的存在可增加液泡色彩的强度并导致&蓝化&。

Methods Chlamydozoan antigen was qualitatively determined using colloidal gold technique. Mycoplasma culture and drug susceptibility were carried out with mycoplasma drug sensitivity kit, and the sensitivity of 10 kinds of antibiotics to mycoplasma was analyzed.

应用衣原体试剂盒定性检测衣原体抗原，支原体培养及药敏选用支原体药敏试剂盒，并对10种抗生素对支原体的敏感性进行了分析。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失