佐剂学

The inactivated tetravalent Riemerella anatipestifer vaccine which contained serotypes SCAU1, SCAU2, SCAU3, and SCAU4 chosen on the basis of epizootiology survey and employed propolis as adjuvant was prepared.

在流行病学调查的基础上，选择华南地区流行广泛的鸭疫里氏杆菌 SCAU1、SCAU2、SCAU3和SCAU4血清型菌株制备蜂胶佐剂四价灭活疫苗。

Recently, it is proved that SP is also involved in pain modulation, acupuncture analgesia and immunoin- hibition. However, the role of CGRP in them is unclear Therefore, the dy- namic changes of CGRP-, SP-like immunoreactivity and expression ofα- CGRP,β-CGRP mRNAs in dorsal root ganglion and spinal cord in the arthritic rats were observed by using immunohistochemistry and in situ hybridization techniques combined with behavioral tests, respectively. Subsequently, the effects of this peoptide on the develoment of polyarthritis were investigated by intrthecal injections of CGRP and its antagonist in the rats. In addtion, GAL is a novel neuropeptide which coexists with CGRP and SP, but it may play a different role in the primary sensory system. Thus, we also observed the changes of GAL and GAL mRNA in DRG neurons and the spinal cord. The results were as follows

为此本文拟在大鼠佐剂性关节炎模型上，结合行为学测定、用免疫组织化学、原位核酸分子杂交技术观察脊髓背角及DRG中CGRP、SP、α-CGRP mRNA、β-CGRPmRNA的动态变化及电针的影响，此外，用鞘内和外周注射CGRP的工具药探讨了CGRP在佐剂性关节炎发生发展中的可能作用；甘丙肽（Galanin，GAL）也是新近发现的一个神经肽，并与CGRP和SP共存于DRG及初级传入末梢中，在神经损伤模型中脊髓CGRP、SP减少，GAL则明显增加，显示GAL与CGRP和SP的作用可能不同，因此在佐剂性关节炎模型上也同时观察了GAL和GAL mRNA的变化，实验结果如下

Methods: The study was designed to observe SS and influence of serum CD_4~+、CD_8~+、IL-2、IL-6、TNF-α, splenic lymphocyte transformation rate, the expression of SS、SSTR-2 in spinal cord、PAG、NRM and hypothalamus the coexistence of SS and enkephalinergic neuron in back corner of spinal cord, the coexistence of SS and 5-HT neuron in NRM by using methods of behavioral nociceptive threshold test, measure of foot's cubage, flowcytometry, MTT, radioimmunity, HPLC-ECD, immunohistochemistry, laser confocal microscope on FAC-induced hyperalgesia.

以弗氏完全佐剂诱发的炎症痛模型大鼠为研究对象，应用行为学痛阈观察、足跖容积测定、流式细胞检测法、MTT法、放射免疫法、高效液相电化学法、免疫组织化学技术法、激光共聚焦技术等方法，观察SS和电针对痛阈、大鼠CD_4~+、CD_8~+、IL-2、IL-6、TNF-α水平和脾淋巴细胞转化率等指标的影响，以及SS、SSTR-2在脊髓和PAG、NRM及下丘脑部位的表达、在脊髓SS与脑啡肽能神经元的共存现象及在NRM中与5-HT能神经元的共存情况。

METHODS: Adjuvant-induced arthritis rats were treated with acupuncture therapy, Chinese herb treatment or both in combination for one week, then the density of TMPH in heart, liver, kidney, and hind paw was determined by HPLC. The cAMP level in the heart and liver was also measured by radioimmunoassay. The blood changes were detected through the test of blood rheometer.

以佐剂性关节炎大鼠为模型，观察单纯针刺法、单纯药物法及针药结合法分别连续治疗一周后，用HPLC法测定各组大鼠心脏、肝脏、肾脏及病灶局部组织中盐酸川芎嗪的含量；用放射免疫法测定各组大鼠心脏、肝脏中cAMP水平；用血液流变仪测定各组大鼠的血液流变学变化。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。