低温实验法

Finally, according to Chinese pharmacopeia (published in 2005) and lots of stable experiments, we can find the best condition for storing GSH is low temperature and dry environment.

采用HPLC法测定产品中GSH的含量，其含量为46.05%，按照中国药典2005年版的规定，对产品进行稳定性实验，确定其为低温、防潮、避光保存为好。

Failure test and displacement evaluation were used to study the biomechanics of reconstruction of ACL by hamstring tendon knot implant fixation in 13, simple tendon knots, and hamstring tendon knot with bone bolt press fit technique in 5 porcine knees.

本研究采用绳肌腱结嵌压固定法和髌腱骨界面螺钉固定法重建交叉韧带，从生物力学的角度来探讨不同材料与固定方法对固定强度的影响。1 材料与方法1.1 实验材料深低温冷冻保存的猪膝关节 35个模拟交叉韧带重建，常温下解冻，取其胫骨、股骨和髌腱骨复合材料和绳肌腱。

Comparatively sundried flowers are more suitable to be extracted as an industrial material although the concrete yield of fresh flowers is a little more. At the same time the experiment process fully proves the selectivity of SFE-CO2. Low temperature and low pressure are suitable to extract concrete from natural flavor and fragrance;(2) The materials prepared to extract rutin are the flowers that are gotten rid of concrete by SFE-CO2. The extraction methods include the SFE-CO2 modified by alcohol, the method of hot alkali-precipitate with acid, and alkaly dissolving and acid subsiding combined with microwave or ultrasonic.

66%；产品运用GC-MS做了鉴定；虽然鲜花浸膏产率较高，但干槐花的物料状态更适合工业生产；同时实验过程充分体现了SFE-CO2的选择性，低温低压条件适合香料的SFE-CO2；(2)以超临界CO2(Supercritical CO2，简称SC-CO2)萃取过浸膏的干槐花为原料，运用夹带剂修饰的SFE-CO2、超声或微波或直接加热的碱溶酸沉法提取中药--芸香甙，相比之下，夹带剂修饰的SFE-CO2和超声辅助的碱溶酸沉法提取效果较好，芸香甙提取率分别为2。

Experiments of HTS research involve a variety of approaches that are crucial to modern physics, for examples, the achievement and control of low temperature, the proper usage of various thermometers and level monitors, the tetrode method of resistance measurement, the identification and elimination of spurious electromotive force and computer data analysis, etc.

低温的获得和控制、各类温度传感器和液面计的特性、电阻的四引线测量法、乱真电动势的判定和消除、数据的计算机处理等，都是物理实验的重要内容。

The toxicity of cryoprotectans increased as the concentration of its increased.3, the survival rate of embryos microinjected with 6M PM was 25.07±1.57% after being coolled in -20℃ for 10 minutes by programmed cooling method, whenas ,the survival rate of controls dealt with five steps balance method was 20.88±2.84%, which indicated that the chilling sensitivity of embryos with microinjection decreased.

而任何一种抗冻剂均随着浓度的增加其毒性随之增加。3、另外，在冷敏感实验中，显微注射6M的PM的胚胎胚胎，应用程序化法处理，以2℃/min的速率降至-20℃，平衡10min后解冻处理，发现注射PM的胚胎低温处理后成活率为25.07±1.57%，而6M的PM五步平衡法同步处理的对照组胚胎成活率为20.88±2.84%，说明显微注射的胚胎冷敏感性一定降低。

The technology of deepfrozen nerve grafts,freezingdrying nerve grafts,frozenirradiated nerve grafts,freezingthawing nerve grafts,are more mature and simpler to apply.However,their effects are also unstable.

单纯深低温冷冻处理、冷冻干燥法处理、冷冻和放射方法处理、冻融处理方法处理等方法技术较成熟、方法简单，但在实验及临床中都存在着不能彻底有效清除抗原问题，使其效果不稳定。

From factorial experiments, it was observed that in conventional extrusion treatment, defatted soy flour, aqueous acidic leached soy protein concentrate and soy protein isolate was able to be texturized, whereas SPC was not.

通过挤压实验可知，在常规的挤压条件下，低温粕、酸法SPC和大豆分离蛋白均可实现组织化，而醇法SPC无法实现有效的组织化。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。