低技术的

On the design of algorithms, a novel exact hierarchical delay analysis method for general circuits is proposed; based on the sensitization theorem for sequential circuits, an exact minimizing clocking method is proposed; based on Boolean process, a waveform simulation method considering interconnecting delay for logic circuit and a parallel waveform simulation method are proposed; a new method that transforms bit-level waveform polynomial to word-level polynomial model is proposed; a multiple valued synthesis algorithm based on multiple valued Boolean process and a wire-centered delay synthesis policy are proposed, in which timing planning, floorplanning, wire planning and optimal clock skew in early design are considered; a two-layers channel routing method for minimizing crosstalk under grid mode is proposed; based on the transition numbers theorems for waveform polynomial, a new method for generation of test with noise effects is proposed.

算法设计方面，提出了一种精确的通用电路层次化延时分析方法；基于时序电路的敏化定理提出时序电路最小时钟周期精确确定方法；提出基于Boolean过程论的考虑互连延迟的逻辑电路波形模拟方法，在分析了波形模拟适合并行化基础上，进一步提出一种并行波形模拟算法；提出一种将位级电路波形多项式描述转化成字级多项式描述的新方法；提出一种基于多值Boolean过程的多值电路综合算法以及一种将前期设计定时规划、前期设计的布局规划和线网结构化方法及低偏移的时钟分配等技术相结合的面向互连延时的综合策略；提出一种串绕最小化的网格模式下的双层通道布线方法；从波形多项式描述跳变数的定理出发提出了一种考虑噪声效应的测试生成新方法。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Many new detection techniques are proposed, that include: To overcome the faults of cross-terms and short unambiguity range for the common Wigner-Ville Distribution , short-time Fourier transform and Gabor transform are applied to SAR moving target detection. To improve the detection performance of WVD in noise environment, a new technique based on the Cross-WVD is proposed. The application of fractional Fourier transform to chirp signal detection is analyzed and extended to SAR moving target detection. To eliminate the restriction of chirp-Fourier transform, a new modified definition is proposed and applied to SAR moving target detection. To detect multiple moving targets whose scattering intensities are different heavily, the CLEAN technique is combined with the above techniques and the ideal detection performance is achieved. At last, the whole dissertation is concluded.

具体的研究成果如下：针对常用的Wigner-Ville分布在多目标情况下存在交叉项，并且多普勒不模糊范围小的缺点，提出将短时Fourier变换和Gabor变换应用于SAR运动目标检测，分析了其优势所在；为进一步提高Wigner-Ville分布低信噪比条件下的信号检测能力，提出基于互Wigner-Ville分布的运动目标检测方法；分析了分数阶Fourier变换在线性调频信号检测中的应用，提出基于分数阶Fourier变换的运动目标检测算法；对离散Chirp-Fourier变换的定义进行了修正，提出修正的离散Chirp-Fourier变换，克服了限制其应用的约束条件，并用于SAR运动目标检测当中；强度相差较大的多运动目标检测历来是机载SAR技术的一个重点和难点，我们将CLEAN思想融入上述多种算法当中，良好地解决了该问题。

The novel PCR technology can accurately categorize the spectrum of FMR1 alleles, including alleles previously considered too large to amplify; reproducibly detect low abundance full mutation alleles; and correctly infer homozygosity in female samples, thus greatly reducing the need for sample reflexing to Southern blotting.

这种新型的PCR技术可以准确判断FMR1基因的类型，包括传统PCR不能扩增的大片段。该法还能检测低浓度的全突变条带，并且重复性好。同时该法还能准确判断女性样本中的纯合子，因此大大减少了需要Southern杂交的样本量。

The invention discloses a multiple address method for communication technology, wherein the overall system bandwidth is sectionalized based on the users' service requirement, and the system sub-segment module allocates the user with corresponding transmitting sub-segments to transmit the user information.

本发明公开了一种新的用于通信技术的多址方法，它是根据用户的业务需求，将系统总的带宽进行分段，由系统子段分配模块为该用户分配相应的传输子段来传递用户的信息。当用户的数据率较低的时候，分配一个传输子段；当用户的数据率较高的时候，分配多个传输子段。

Today the company's International Combustion Division has developed close working relationships with the leading companies in this industry, and can demonstrate technology which maximises both hydrocarbon processing performance and environmental protection.

联迈国际的核心专利是&THERMIMAX&燃烧混合锥，该混合锥能够使燃烧设备具有更高效率的，更低的排放以及更稳定的燃烧。由于客户对我们&THERMIMAX&专利技术的应用，使他们在日趋激烈的商业竞争中取得了领先的地位。

On the basis of investigating the resource and utilization of Iris plants in Nanjing, Hangzhou and Wuxi, the paper selected five plants: Iris pseudacorus、 Iris sanguinea、 Iris kaempferi、 Iris japonica、 Iris tectorum to find their tissue culture technology and shade tolerance with lots experiments. The results were summarized as follows:In the culture of Iris sanguined, The shooting tip in the spring and the medium supplemented with 6-BA(1mg/L)+NAA(0.2mg/L) testified the best grouping.

本文在对南京、杭州、无锡等地鸢尾属植物及其园林应用实地调查的基础上，以黄菖蒲、溪荪、花菖蒲、蝴蝶花、鸢尾等五个种为实验材料，从其部分种的组织培养技术和耐荫性两方面入手，通过大量的实验并对所得数据进行统计分析，得出以下结论：溪荪的组织培养中，以春季萌发的茎尖为外植体，以MS培养基附加低浓度的6-BA(1mg／L)与NAA(0.2mg/L)为最佳的诱导分化的组合。

We often our product under the high quality and low cost in timely manner through the mass-production system. JEIL MTECH is certified for ISO 9001 and many kinds of domestic certificate, which are being proof of best technology and well-constructed quality control system.

我们的产品是高质量低成本的，并及时的通过了ISO9001的鉴定，还获得了许多国内的证书，这些都是经过了严格的技术考验，并拥有了很好的质量控制体系。

Their basic instruments are the marimba, traditional Islamic instruments such as lira gabbang , the low toned bumbong and panpipes , as well as melodic angklungs .

创立於1973年，由马尼拉菲律宾综合技术大学的50位大学生组成，乐团所使用的乐器都是采用当地材料手工制成，最基本的乐器包括悦耳的marimba、传统回教乐器lira gabbang、低沈的bumbong和panpipes，以及提供配合旋律的angklungs。

The NTN each kind of bearing product, is produces take 0.01 micron as unit's precision work and the examination technology, and requests the low cost from both the request high performance at the same time the ordinary domestic electric appliances, still could display its bearing function normally until the request in the severe working conditions the astronavigation rocket, NTN bearing all the various trades and occupations, each use machinery development built the foundation again.

NTN 的各种轴承产品，是以 0.01 微米为单位的高精度加工和检测技术生产出来的，从既要求高性能同时又要求低成本的普通家用电器，直到要求在严酷的工作环境中仍能正常发挥其轴承功能的宇航火箭为止， NTN 轴承再各行各业、各种用途机械的发展打下基础。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。