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Results:Part1: Mean serum EPO concentration of CRF patients showed significant lower than that of control. After transplantation, renal function recovered quickly while Hb level increased steadily in 37 patients with immediate graft function. Their EPO concentration increased significantly and double peaks of EPO level were obtained at day 4 and week 4. 4 cases in IGF suffered from post-transplant erythrocytosis. But their EPO concentration did not reach statistical significance. 5 cases developed delayed graft function, without EPO double peaks and their EPO concentrations showed signiffcant lower than that of IGF.

结果:第一部分:尿毒症病人血清EPO浓度明显低于正常对照值。37例立即肾功能恢复者,SCr迅速下降,Hb上升,其血清EPO浓度在术后第4d形成第一个高峰,与术前和正常对照比较均有显著差异,其后开始下降,在术后4w时再次升高形成第二个高峰。4例IGF患者在术后16w左右发展为PTE,其EPO浓度值略增高,但与IGF组患者EPO水平无统计学差异;5例DGF患者术后无典型的双峰特点,EPO水平明显低于同时期IGF组,直到肾功能恢复后才逐渐升高。

The results showed that at the onset of this disease, the serum zinc concentration of 20 patients was lower than the limit of the normal value, and the mean concentration of serum zinc in patients was significantly lower than that of the control group.

在 4 1例白血病患儿和 3 3例正常对照儿童中,观察到在明确诊断化疗前,4 1例白血病患儿中有 2 0例患儿的血清锌浓度低于正常低限,他们的平均血清锌浓度为 1 2 3± 3 4 μmol/L ,明显低于对照组 1 8 3± 4 0 μmol/L。

MethodsTwenty volunteers with normal hearing level and tympanic pressure were enrolled and kept at airtight container. The inner pressure was changed to 2 kPa and 4 kPa below normal atmospheric pressure in 50 s and recovered to the normal pressure, the pure tune hearing threshold of 500, 1 000 and 2 000 Hz and tympanic pressure were measured, and acoustic impedance curve characteristic and tympanic membrane appearance were recorded at each time points.

方法选取听力和鼓室压力正常的受试者20例,即单耳40耳,评估其于50s改变密闭模拟电梯容器压力低于正常大气压2kPa、50s改变密闭容器压力低于正常大气压4kPa、以及压力恢复至正常水平时,言语频率500、1000、2000Hz的纯音听阈,鼓室压值和声阻抗曲线图形形态,以及鼓膜形态。

There was no statistically significant difference in parameters of IVS motion between obstructive and nonobstructive HCM.The data of hypertrophic LVPW motion were lower than those of normal subjects,as well as those of nonhypertrophic LVPW in HCM group.

左室后壁肥厚者收缩期及舒张早期的DTI测值多低于正常,而左室后壁不肥厚者收缩期指标无明显变化,舒张早期指标低于正常;两者相比,前者收缩期及舒张早期各参数多低于后者。

Results 1. The expression of P53, KI-67, hTERT and ToPo II a protein was higher in astrocytoma than that in nomal brain tissue, the expression of these markers was obversely correlated to the grade of astrocytoma (P.05). The expression of Fas was higher in astrocytoma than that in nomal brain tissue, on the contrary, expression of P16 was lower in astrocytoma than that in nomal brain tissue.

结果 1、星形细胞瘤中P53、KI-67、hTERT与ToPoⅡα蛋白表达阳性率高于正常脑组织,P53、KI-67、hTERT与ToPoⅡα蛋白表达与星形细胞瘤的病理组织学分级呈显著正相关(P.05);Fas蛋白在星形细胞瘤中的表达高于正常脑组织,而P16蛋白表达低于正常脑组织,Fas、P16蛋白表达与组织学分级无显著相关性。

To test the hypothesis, neontal rat cadiomyocytes Treat with heat shock(HS,42℃,2h) to induce the expression of HSP70,then teated with 0.5mmol/L H2O2. We first found H2O2 can reduced cadiomyocytes apoptosis and HSP70 only over-expression in the HS group, other two group only express HSP70 a little in the test of expression of HSP70.Then we tested the concentration of Ca2+, the [Ca2+ ] of CON was 192.224+6.654, the [Ca2+ ] of H2O2 group was 290.6918+8.922and the [Ca2+]of HS group was 214.2633+4.484.To further determine how HSP70 repaired the Ca2+ homestasis, we measured calcium transient of each group.

实验中应用MTT和流式细胞技术检测细胞的凋亡,发现损伤组的存活率明显低于其他2组,热休克组(Heat shock group,HS group)细胞存活率略低于正常组细胞;免疫组化结果表明只有在HS组才检测到阳性反应,即是HSP70的表达,其他2组均为弱阳性结果;应用离子成像技术对细胞内钙离子浓度进行测定,发现损伤组290.6918+8.922明显高于正常组192.224+6.654和HS组214.2633+4.484,HS组细胞内的钙离子浓度略高于正常组;为了进一步探讨HSP70对于ROS引起的心肌凋亡过程中钙离子的调控机制,又对各组的心肌细胞进行了钙瞬变的测定。

Results:① The PO2 of model group reduces obviously, but the PCO2 raises obviously, compared with normal contral group having notable difference; The PCO2 of every curing group is lower than the normal contral group equally, the PCO2 overtops the normal contral group obviously, and having notable difference. It explains that the model is maded successful.

结果:①模型组的 PO2明显降低,而 PCO2明显升高,与正常组相比有显著性差异;治疗各组 PO2均低于正常组, PCO2明显高于正常组,且有显著性差异,说明造模是成功的。

Results In pre-school children, tibial and radialis bone strength of obese group was worse than normal weight group, and the descending risk of bone strength in obese group was 6.87 and 2.24 times of normal children. Bone strength was not different in overweight and normal weight groups.

结果 学龄前肥胖儿童胫、桡骨骨强度均低于正常儿童,胫、桡骨骨强度下降风险分别是正常儿童的6.87倍和2.24倍;超重组与正常组无差别。

In school children, tibial bone strength of obese group and overweight group, normal weight group was in the increasing order; the descending risk of bone strength in obese and overweight group was 9.73 and 2.54 times of normal group respectively. Radialis bone strength of obese group was worse than overweight group and normal weight group, while the bone strength of overweight group and normal weight group was not different.

学龄儿童正常、超重、肥胖组的胫骨骨强度依次下降,肥胖、超重组胫骨骨强度下降风险是正常组的9.73和 2.54倍;学龄肥胖组桡骨骨强度低于正常组,超重组与正常组无差别。

The results showed that (1) COX-2 mRNA and protein existed in the mature male rat testis. COX-2 was localized in spermatocytes by immunohistochemistry.(2) COX-2 also existed in adult man testis. COX-2 protein was positively stained in spermatocytes and Leydig cells.(3) 2 weeks after administration of rofecoxib, the level of testosterone in the whole testis reached its lower values, being only 50% of control values. However, testosterone level recovered during 4 weeks. After such treatment, histologic examination of these testes showed atrophy of the seminiferous tubules and maturation arrest of spermatogenesis.(4) 2 weeks post-EDS, expression of COX-2 decreased significantly (P.005), in comparison with vehicle-treatment control. 4 weeks after treatment, a new generation of fetal like Leydig cells repopulated in the testicular interstitium resulting in COX-2 expression partially recovered. Although the expression of COX-2 mRNA and protein are enhanced at 8th week after using external testosterone, it wasn't significant higher than control group.

实验研究证明:(1)正常成年雄性大鼠睾丸组织中COX-2在mRNA和蛋白质水平均存在表达,免疫组织化学结果显示COX-2定位于曲细精管内的生精细胞;正常成年男性睾丸组织中同样存在COX-2表达,免疫组织化学结果显示COX-2定位于曲细精管内的生精细胞和Leydig细胞;(2)服用特异性COX-2酶抑制剂rofecoxib 2周后,实验组大鼠睾丸组织内睾酮的含量减少,为正常对照组的50%;持续用药4周后睾丸组织内睾酮浓度逐渐恢复至正常水平;COX-2酶活性降低后病理组织切片显示睾丸内曲细精管萎缩,生精紊乱,持续用药4周时影响最明显;(3)注射特异性Leydig细胞杀灭剂EDS 2周后,实验组大鼠睾丸组织内睾酮浓度降至极低水平时,COX-2的蛋白和基因表达水平也显著低于正常空白对照组(P.005);使用EDS后第4周,睾丸组织中的睾酮浓度逐渐回升,同样组织中COX-2蛋白表达和mRNA表达水平也相应提高接近正常水平;使用EDS后4周开始给予外源性睾酮,6周和8周时COX-2表达水平的绝对值虽有提高,但与正常大鼠空白对照组比较并无显著性差异,说明外源性雄激素刺激睾丸合成COX-2的作用并不明显。

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