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The results showed that:The reductions in the shoot and root dry weights, leaf area,stern diameter of grafted plants were lower than those of ungrafted plants under the same NaCl stress.Na~+,MDA,H_2O_2 contents,O_2~- producing rate,Na~+/K~+ ratio, Na~+/Ca~(2+) ratio were lower,whereas K~+,solube sugar,shoot water content,SOD,POD, CAT,APX activity,the stability of AsA/DHA,Photosynthetic rate,transpiration rate and stomatal conductance in the leaf of grafted plants were higher than those of ungrafted plants at the same NaCl stress.Intercellular CO_2 concentration of grafted plants decreased whereas that of ungrafted plants increased significantly under salt stress.

结果表明,盐胁迫下,嫁接苗地上部和根干重、叶面积、茎粗受抑制的程度显著低于自根苗;嫁接苗叶片中Na~+、MDA、H_2O_2含量、超氧阴离子产生速率、Na~+/K~+和Na~+/Ca~(2+)比值显著低于自根苗,而叶片中K~+含量、可溶性糖含量、地上部含水量、SOD、POD、CAT、APX活性、AsA/DHA的稳定性、光合速率、蒸腾速率、气孔导度显著高于自根苗;嫁接苗的胞间CO_2浓度显著下降,自根苗的则显著上升。

In conclusions: 1 The treatment that buffalo occytes are cultured in Ionomycin (5 min)+CDM (3 h)+ 6-DMAP(3 h) after ICSI with sorted sperm can improve their subsequent embryonic development; 2 The dead ultrasoniced sorted sperm can be used for ICSI; 3 The result of ICSI with sorted sperm is similar to that with unsexed sperm, but the result of IVF with sorted sperm is lower than that with unsorted sperm; 4 The treatment of heparin after ICSI with sorted sperm can improve the blastocyst development rate; 5 The development rate of blastocyst with sorted sperm was variable among buffalos indicating that sorting high quality sperm from specific buffalos can increasethe efficiency of ICSI.

结果表明:1水牛分离精子ICSI后用Ionomycin(5min)+CDM(3h)+6-DMAP(3h)方法激活处理能提高其胚胎发育率;2分离后的死精子经超声断尾也能用于水牛卵母细胞的ICSI;3分离精子的ICSI效果不低于未分离精子,但分离精子的IVF效果却低于未分离精子;4肝素能促进分离精子ICSI后的胚胎发育;5分离精子ICSI后的胚胎发育率在不同公牛之间有差异,选择能产生高质量精子的公牛进行精子分离,可以提高ICSI的效率。

The results show that there were no significant differences among the four forage rations in rumen pH, the concentrations of total volatile fatty acids, acetate, propionate, the ratios of acetate to propionate, butyrate, isobutyrate and valerate(P﹥0.05). Only in 11:30, the concentrations of isovalerate caused by ration A and B were significantly higher than the other two rations(P﹤0.05).The milk yield caused by the ration C was significantly lower than the others (P﹤0.05). However, there were also no significantly differences among these four rations in the DM, fat, sugar, protein and scc of the milk. The milk fat and sugar of ration B and C were higher than ration A and D in number as well.

试验结果显示:各粗料组合日粮对各个时间点的瘤胃发酵指标:pH、TVFA、乙酸、丙酸、乙酸/丙酸、丁酸、异丁酸、戊酸浓度均无显著差异(P﹥0.05),只在11:30时,日粮A、B的异戊酸浓度显著低于日粮C、D (P﹤0.05);日粮C的产奶量显著低于其它三个日粮,而在乳干物质、乳脂、乳糖、乳蛋白、体细胞数上,各日粮均无显著差异,从数值上看,日粮B、C的乳脂和乳糖高于A、D。

objective to study gastrodia elata blume to effect tau protein,sod,mda expression in gyrus hippocampi and cerebral cortex of experimental mice.methods to inject okadaic acid to mice ventriculus lateralis and to measure tau protein level,sod activity and lipid superoxide mda content of sod control group,oa injection group,oa and gastiodia elata injection solution group.results tau protein of experimental group(p<0.05/p>0.05),sod was lower than model group(p<0.001) and was higher than control group(p<0.05).conclusion gastrodia elata blume can increase sod activity and reduce tau protein expression and superoxide lipid forming in brain tissue of experimental dementia mice caused by oa.it can prevent and treat ad.

目的 观察天麻对痴呆模型大鼠海马、皮质神经元微管相关蛋白、超氧化物歧化酶和脂质过氧化物丙二醛表达的影响,探讨其治疗阿尔茨海默病(alzheimer disease,ad)的作用机制。方法用冈田酸(okadaic acid,oa)注射大鼠侧脑室造模,测定模型组、实验组、对照组海马和皮质tau蛋白、sod、mda的含量。结果实验组tau蛋白低于模型组(p<0.001),高于对照组(p<0.05);sod高于模型组(p<0.001)和对照组(p<0.05);mda低于模型组(p<0.001),高于对照组(p<0.05)。结论天麻可增强oa致实验性痴呆大鼠脑组织sod活性,降低mda蓄积和tau蛋白生成,具有防治阿尔茨海默病的作用。

Article 20: The wage of a worker during his/her probation period may not be lower than the lowest rung on the wage scale for the same position with the Employer or 80% of the wage specified in the employment contract, and may not be lower than the minimum wage rate in the place where the Employer is located.

第二十条劳动者在试用期的工资[y13] 不得低于本单位相同岗位最低档工资或者劳动合同约定工资的百分之八十,并不得低于用人单位所在地的最低工资标准。

Result After addition of wood vinegar, the fermentation temperature of sawdust was obviously increased, and the highest temperature reached 67.0℃,being 3.5℃ higher than that of CK, and the days when fermentation temperature was above 55℃ reached 57 d, being 40 d more than that of CK. After addition of wood vinegars, the bulk density, water-holding voidage and total voidage of sawdust before and after maturity were all higher than that of CK, pH value and EC value were all lower than that of CK, and the proportion of sawdust whose particle size was smaller than 0.5 mm was higher than that of CK, and that of sawdust whose particle size was 0.5-1.0 mm and bigger than 1.0 mm were lower than that of CK.

结果]添加木醋后,木屑的发酵温度明显提高,最高温度为67.0℃,比对照高3.5℃,发酵温度大于55℃的天数达到57 d,比对照多40 d;添加木醋后,木屑的容重、持水空隙度和总空隙度腐熟前后均高于对照,pH值和EC值均低于对照,粒径<0.5 mm的木屑所占比重高于对照,粒径为0.5~1.0 mm和>1.0 mm的木屑所占比重均低于对照。

The ISLQ scores of cognitive component,emotional component and total scores were significantly higher than control group. The scores of all dimensions in research group were lower than control group. And the scores of family life,affiliate with accompanier,school life,self-cognition,body emotion and anxiety experience were significant difference in two groups.

焦虑组ISLQ评分在认知成分、情感成分、总分上均低于对照组,差异有统计学意义;两组8个维度水平的比较中,各维度的得分焦虑组均低于对照组,除了生活环境、抑郁体验维度外,其余家庭生活、同伴交往、学校生活、自我认识、躯体情感、焦虑体验6个维度差异有统计学意义。

Different strains have different reaction to the delay of oviducts in body after animal death. After 10min delay C57BL/6 mouse oocytes have death rate of 56.5%,significantely higher than Kunming mouse (47.6%);Spontaneous activation rates were respectively 13.3% and 46.0%, C57BL/6 were obviously lower than Kunming mouse.4. The oviducts were obtained after being delayed 5min 24h after the mice were injected with hCG. The oocytes were cultured in CZB. About 81.1% occurred spontaneous activation, evidently lower than parthenogenetic rate (96.4%) with SrCl_2. Spontaneous activable oocytes had high cleavage rate(93.2%) and 4-cell rate(87.3%). However, spontaneous activable oocytes had blastula development rate(18.7%) as low as parthenogenetic oocytes by SrCl_2(22.9%).

不同品系小鼠卵母细胞对输卵管在体内滞留产生的反应不同,滞留10min C57BL/6系小鼠卵母细胞死亡率为56.5%,显著高于昆明鼠(47.6%);自发激活率分别为13.3%和46.0%,C57BL/6系小鼠显著低于昆明鼠。4.hCG后24h体内滞留5min卵母细胞在CZB中培养自发激活率为81.1%,显著低于SrCl_2孤雌激活率(96.4%);自发激活的卵母细胞有较高的卵裂率(93.2%)和4-cell比率(87.3%),但囊胚率(18.7%)较低,同卵龄的卵母细胞经SrCl_2孤雌激活囊胚发育率为22.9%,差异不显著。

RESULTS:In the serum,SOD activity of all the nm-haFGF groups and the haFGF group increased significantly while the content of MDA decreased dramatically compared with the model group;The content of BUN and Cr aland haFGF group rose significantly compared with the model group,while MDA decreased dramatically.

结果:缺血再灌注24 h后,nm-haFGF所有剂量组和haFGF组血清SOD活性明显高于模型组,MDA含量明显低于模型组,而血清和尿BUN和Cr含量均明显低于模型组;肾组织SOD活性在nm-haFGF 20 ìg/kg和40 ìg/kg剂量组和haFGF组明显升高而MDA含量明显降低。

Group T has the same tendency as group B; but the degree of alveolitis and pulmonary fibrosis decreased. Compared to group B, the expression of TGF-β1, Smad3 was decreased significantly in group T, so did the index of lung cells apoptosis.

干预组各时间点TGF-β1和Smad3表达明显低于模型组(P.05),28天时大鼠肺组织中细胞凋亡指数干预组明显低于模型组。

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