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An amount increase ofPAI-3 andK10 was observed after theKCswere cultured for48 h in high calcium medium,butat72 h the exprssion ofPAI-3 decreased. The positive stain ofPAI-3 wasmainly localized in perinuclearmembrane in lowcalcium medium and in cytoplasm in high calcium medium. PAI-3 was incorporated into cornified envelope ofterminally differentiated keratinocytes.

结果 高Ca2+浓度下培养24 h后，分化标记物K10弱阳性表达， PAI-3呈阳性表达；培养48 h后， K10强阳性表达，而PAI-3的阳性表达明显增加， 72 h后，表达开始降低，在低Ca2+浓度下阳性染色主要位于细胞核膜周围而高Ca2+浓度下主要位于细胞质，同时， PAI-3存在终末分化KCs角质壳中。

And cribriform roof is behind it. On endoscope, we can see anterior ethmoid artery is in the insertio of basal lamella of bulla and frontal sinus, in about 2mm range. They are all behind along ostium of frontal sinus and in front of the basal lamella of bulla, from posterolateral skull base to anteromedial skull base.

鼻内镜下，筛前动脉位于筛泡基板与颅底附着处、前后2 mm范围内，沿颅底由后外向前内走行，其行程始终位于额窦开口后缘的后方和筛泡基板的前方。

As for sulfur isotopic composition, the general feature of two types deposits is a single peak distribution. All δ〓s values of the ores are near zero. The difference of different deposits lies in that the δ〓s values (1. 8-5. 2‰) of LDT are of small positive value and are similar to that of the metamorphic rocks and dioritic porphyrites in the region while the δ〓s values of SDT are always near zero. Features above suggest that two types of deposits have closely genetic relationship to metamorphic and magmatic rocks.

硫同位素组成上，两类金矿床共同的特征是均具有明显的单峰塔式效应，且δ〓S值均位于0值附近不大的变化范围内；不同的是，近程式金矿的δ〓S平均值均接近于0，与该类金矿具密切成因联系的岩体的δ〓S值也接近于0值，而远程式金矿的δ〓S平均值均为小的正值，变化范围为1.8～5.2‰，区内太古代变质岩及矿区内闪长玢岩脉的全岩δ〓S值位于这一区间内，反映了远程式金矿床其成矿物质不仅来源于重熔岩浆，同时还可能部分来源于变质岩围岩。

The S type of CMS is a gametic sterility type, and its pollen sterility was considered to be associated with the expression of R region in the mitochondria.

其中两个含有完整R区的BamHI片断已被克隆测序，结果表明在这两个片断中cox1和cox2分别位于R区上游，但与之位于不同的极性单链上。

Results: the location, shape and number of the sympathetic ganglia were quite variable, and there was 1 to 6 in number unilaterally. Lumbar sympathetic trunk divided into 2-3 branches at the distal part were found in 12 sides. Lumbar arteries were all located under the lumbar sympathetic trunk and the lumbar veins, consisting (68.42+4.35)% situated superficially to the trunk, mainly in L4 arid L5 veins. Genitofemoral nerve pierced psoas muscle at the level of L2-L4 or its intervertebral disc (0.83±0.48) cm away from the medial edge of psoas muscle.

结果：腰交感神经节数目及位置变异较大，每侧1~6个不等；腰交感干可分裂为2~5支，分裂处多在干的下端，本组共发现12侧发生分裂；腰动脉均位于神经干的深面，腰静脉位于腰交感干浅面者占(68.42±4.35)%，以腰4、5静脉多见；生殖股神经穿出腰大肌平面多数与第2~4腰椎或椎间盘对齐，穿出点距腰大肌内侧缘(0.81±0.48)cm。

The data byχ~2 were consistent with the separation ratio as 1: 2: 1 χ~2=4.028＜χ_(0.05,2~2=5.99, which further conformed that glandless character was controlled by a incomplete dominance gene, Gl_2~e. 2. SSR markers every about 5cM were selected in the chromosome 12 of our lab genetic linkage map to screen the parent plants. The markers with the polymorphism were used to amplify the DNA of 210 F_2 segregating plants selected randomly and recorded the marker genotypes. After eliminating the segregation distortion markers byχ~2 test, other SSR markers were detected by linkage analysis.

二、参照本实验室海陆种间遗传连锁图谱第12染色体上的SSR标记，平均每5cM左右选择一个标记进行亲本多态性的筛选，筛选出具有多态性的标记位点进一步扩增随机抽取的210个F_2群体单株的DNA，鉴定每个个体的标记基因型。X~2检测剔除偏分离标记后进行连锁分析，发现Gl_2~e基因位于CIR362与NAU5079之间，进一步参考图谱上位于CIR362与NAU5079之间的标记加密图谱，最终将其定位在CIR362和NAU2251b、NAU3860b、STV033之间。

Handrailing shall be a two-rail system with the top rail 42 inches (1.07 m) above the walkway surface and the intermediate rail 21 inches (53.3 cm) below the top rail.

扶手应该采用双轨道系统。顶栏位于人行道表面以上42英寸（1.07m），中栏位于顶栏以下21英寸（53.3厘米）。

The structure consists of discrete adamantylammonium cations lying on crystallographic mirror planes and hexa- fluoridogermanate anions disordered about sites of 2/m point symmetry.

它的结构中包含位于晶体学对称面上的离散的金刚烷胺阳离子，而无序的六氟锗酸盐阴离子位于2/m对称点。

There are 11 gene bindings disappear in the normal group, but appear in the model group, and then disappear in the treat group, that indicates the 11 gene bindings are correlated with the epilepsy and Caohuozhimutang. After searching in the Gene Bank, 7 bindings in the 11 gene bindings are homogenic with the 8q31 ribosomal protein gene, 15p12 paraneoplastic neuronal antigen gene, 4q22 diacylglycerol kinase iota gene, xq37 FMR2 protein and 11p11 roudabout homolog 1, and 4 bindings of which are novel gene.

经NCBI美国国家基因库检索，这11条基因中的7条已知基因分别与位于鼠染色体8q31位置的核糖体蛋白、15p12位置的周围肿瘤神经元的抗原(paraneoplastic neuronal antigen)、4p22位置的甘油二酯酶、xq37位置的FMR2蛋白（FMR2 protein）的基因以及以及位于果蝇染色体11p11位置的交叉同族体（roundabout homolog 1）有极高的同源性；有4条基因片段同源性较低，为功能未知的新基因，已经在GeneBank进行了注册，注册号分别CK325391、CK325392、CK325393、CK325394。

Mutant libraries created by saturation mutagenesis of each single amino acid site D168, A225, K434 and E435 were screened to further improve the capability of cytochrome P450 BM-3(A74G/F87V/L188Q) mutant from Bacillus Megaterium which can hydroxylate indole into indigo. Results showed that D168, K434 and E435 are located at the functional domain of P450 BM-3 protein while A225 is sited at the unfunctional domain.

为了进一步获得具有更高活力的细胞色素P450 BM-3(F87V/A74G/L188Q)突变酶，利用饱和突变技术对该突变酶的4个氨基酸位点D168、A225、K434、E435分别进行单一的随机突变，通过对其羟基化吲哚生成靛蓝的催化性能表征，发现P450 BM-3氨基酸残基的168、434和435位均位于蛋白功能区域，而225位则位于非功能区域。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷