伴侣

And of course, like their historical soul-mates, they spent years denying the crisis even existed, denouncing critics as alarmists.

当然，这些家伙也和他们历史的心灵伴侣一样，历经多年仍否认经济危机的存在，认为危机只不过是危言耸听罢了。

It is the most patient and cheerful of comp anions.

它是最有耐心、最令人愉快的伴侣。

Poicephalus parrots and caiques cope much better than almost all other species at living with people as companion birds.

波依塞佛拉鹦鹉和凯克鹦鹉养起来，几乎比所有鸟类作为人们的生活伴侣更好。

Overall the ATTACH3D combines the versatility of a mini-HMS carabiner in a compact, light frame for fast and light adventures.

ATTACHE 3D集合了迷你HMS锁扣的各种功能，超轻的框架结构是您探险途中的最佳伴侣。

Or you may date chameleons, choosing partners who conform to your personality.

或者你会和变色龙约会，选择一个合你胃口的伴侣。

INSPECT YOURSELF AND YOUR PARTNER Inspect yourself for a discharge, bumps, rashes, warts, blisters, chancres, sores, lice, or foul odors.

检视自己以及伴侣一旦有任何异常的分泌物、肿块、疹子、疣、水泡、下疳、疮、溃疡、虱子或异味，你就必须做检验。

With the assistance of the chaperons such as calnexin、calreticulin and tapasin,the molecular heavy chain andβ2 micro globulin of the major histocompatibility complexare assembled together to form the steady complex which is further transported to the surface of the APC through the Golgi body.

在calnexin、calreticulin、tapasin等分子伴侣的协助下，内质网腔内的主要相容性复合体(major histocompatibilitycomplex，MHC)Ⅰ类分子重链、β2微球蛋白以及相应多肽片段聚集成较为稳定的复合物，经高尔基体被进一步转运到APC表面；MHCⅠ类分子与抗原肽的有效结合是特异性细胞免疫应答的重要的环节。

The objectives of this study are:(1) To amplify Tb wbbL gene encoding rhamnosyl transferase bypolymerase chain reaction from the genomic DNA ofM.tuberculosis H37RV strain;(2) To clone PCR product of Tb wbbLgene into a cloning vector pMD18-T for sequencing;(3) To subcloneTb wbbL gene to an expression vector pET16b to construct pET16b-Tb wbbL; and to overexpress Tb WbbL protein in E.coli BL21(DE3)under different induction conditions;(4) To establish theco-expression system for expressing chaperons of pKJE7 plasmidand soluble Tb WbbL protein in E.coli BL21(DE3) under differentinduction conditions;(5) To test expressed WbbL protein by SDS-PAGEand Western blot methods.

本论文的目的是：(1)利用 PCR 方法从结核分枝杆菌 H37Rv 菌株的基因组DNA 中扩增出 Tb wbbL 基因；(2)将 Tb wbbL 基因克隆到pMD18-T 克隆载体中，经 DNA 序列测定证实为正确的基因；(3)将 Tb wbbL 基因亚克隆到 pET16b 表达载体中并通过改变不同的诱导条件在大肠杆菌 BL21(DE3)中表达 Tb WbbL 蛋白质；(4)建立在 BL21(DE3)大肠杆菌中共表达 Tb WbbL 蛋白质与分子伴侣的体系，优化表达条件以高效表达出可溶性 Tb WbbL蛋白质；(5)用 Western blot 方法鉴定所表达的 Tb WbbL 蛋白质为 Tb wbbL 基因产物。

Women are now considered as equal partners, not as chattels or housekeepers.

如今妇女已被视为平等的伴侣，而非私有财产或家庭主妇。

My future mate will have hair the color of chestnuts.

我未来的伴侣将会有茶色的头发。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。