伤寒沙门氏菌

More than 90% of the total incidence of typhoid and paratyphoid were typhoid previously, as the widely use of Vi bacterin, in the middle of 90s in 20th century, the popular strain changed, in some provinces the cases of typhoid decreased, but that of paratyphoid increased largely, with the outbreak trend from spot to part and from inshore provinces to backland provinces. In recent years, paratyphoid becomes the main infectious diease in some areas, and ofen develop to serious epidemic situation. So setting up a rapid and accurate detecting metod is necessary.

以前我国伤寒发病占伤寒副伤寒总发病的90%以上，后来由于伤寒Vi疫苗的广泛使用，20世纪90年代中期，流行菌株发生转变，我国部分省份伤寒病例逐渐下降，而由甲型副伤寒沙门氏菌引起的副伤寒病例却大幅上升，并呈现由散发到局部暴发，由沿海省份向内地省份推进的流行趋势，近几年来成为我国一些地区传染病突发事件的主要病种，常发展成重大疫情，急需建立快速、准确的诊断方法。

It was estimated that 1.4 million human cases with non-typhoidal Salmonella infections, and probably 800-4000 related deaths occur in the United States annually. In terms of public health, avian salmonellosis is a hazard to cause food poisoning cases in humans.

沙门氏菌症为重要的人畜共通传染病，美国每年约有、40万人以上感染非伤寒性沙门氏菌症，造成800-4000件死亡案例，以公共卫生的观点，家禽感染沙门氏菌最易引起人们食物中毒。

Alternatively, if the precis is to include a mention of methodology, it might read as follows,"Using ground meat from a variety of commercial sources and standard conditions for the culture of bacteria, we showed that refrigeration failed to prevent food poisoning by hamburgers contaminated with Salmonella typhimurium."

二者择一地，如果摘要是包括方法学的提及，它也许如下读，"使用绞肉从各种各样的商业来源和标准条件为细菌文化，我们表示，冷藏没有由汉堡包防止食物中毒沾染与鼠伤寒沙门氏菌"。

A bacteriocin-like substance producing strain T12 was screened from 212 lactic acid bacteria isolated from intestine of healthy pigs by paper disk test and was identified as Lactobacillus. The supernatant of T12 exhibited strong inhibitory activity against the gram-positive bacteria, including Micrococcus lysodeikticus, Staphylococcus aureus, Bacillus subtilis and Bacillus cereus. A strong activity was also observed against the gram–negative bacteria and some eumycete, such as Escherichia coli, Salmonella typhimurium and Fusarium graminearum.

通过滤纸片法从健康肉猪猪大肠、小肠中分离得到212株抗生物质产生菌，以杯碟法复筛，得到1株对溶壁微球菌、金黄色葡萄球菌、枯草芽孢杆菌、蜡状芽孢杆菌等革兰氏阳性菌和大肠杆菌、鼠伤寒沙门氏菌等革兰氏阴性菌以及部分真菌如禾谷镰刀霉均有强烈抑制作用的乳酸菌。

The Taenia solium oncosphere 18 gene was cloned and reconstructed into expression vector pYA3341.The recombinant plasmid pYA3341-TSOL18 was finally electro-transformed into an attenuated S.typhimurium definitive host strain of X4500 and the recombinant strain of X4500 (pYA3341-TSOL18) was identified its immunogenicity of expression protein, stability, growth curve, safety in vitro and immunity evaluation by animal experiment in mouse.

克隆并改造TSOL18基因，构建重组质粒pYA3341-TSOL18，电转入鼠伤寒沙门氏菌终宿主菌株X4550，体外鉴定重组菌X4550(pYA3341-TSOL18)表达蛋白的免疫原性、稳定性、生长曲线、安全性和小鼠免疫试验进行评价。

In order to examine the safety of Evening Primrose Oil Capsule in toxicology, a series of tests are performed, including the acute peroral toxicity test in mice, Ames test, PCE micronucleus test in Bone Marrow cells on mice, sperm abnormality test on mice and 30 days feeding test on rat.

为了探讨其毒理学安全性，本研究利用小鼠急性经口毒性试验、鼠伤寒沙门氏菌/哺乳动物微粒体酶试验、小鼠骨髓嗜多染红细胞微核试验、小鼠精子畸形试验、30天喂养试验等方法对月见草油胶囊进行了食品安全毒理学检测。

We used the immunohistochemical ABC method to detect the expression of NF-κB at 2、 6、 12、 24、 48、 72 hours after infected by Salmonella typhimurium, and we deal the data statistically with univariant analysis of variance and Student-Newman-Keuls multiplerange test.

三组分别在感染鼠伤寒沙门氏菌后2、6、12、24、48、72小时6个时点，用免疫组织化学方法(抗NF-κB p65抗体、ABC法，石蜡包埋)和阳性细胞面积分布率半定量测定小肠、结肠及肾脏中NF-κB活性表达百分率，采用单变量多因素方差分析和Q检验做统计学处理。

The mutagen mechanism is presumed single factor dominant.(4)There was 85 percent transvertion in mutagen spectra, and they were all G:C→T:A transvertion. The maximal possibility of mutagen mechanism is analyzed as followed:ion beam implant → breakage cell surface and enter the interior → stimulate plentiful radical born → radical oxidize and damnify DNA → G becomes 8—oxoG → 8—oxoG pairing A → result in G:C→T:A transvertion(5)Production of transition was presumed that ion beam implantion damnified DNA, stimulate activity of MMR interveniented by methyl reducing.

诱发突变谱中碱基颠换比例是0.85，且是全部G：C→T：A颠换，推测低能离子束诱变鼠伤寒沙门氏菌得分子机制可能为：离子注入→通过破坏细胞表面进入内部→刺激产生大量自由基→自由基氧化损伤DNA→G变成8氧—7，8—二氢鸟嘌呤(8—oxoG)→8—oxoG与A配对→导致G：C→T：A颠换。

Typhimurium TA 98 and TA 100. 38 kinds of soy sauce showed significant direct-acting mutagenicity toward 2 strains without S9 mix; and other 2 kinds presented indirect-acting mutagpnicity with S9 mix.

亚硝酸盐浓度50 mmol/L时，38种酱油对鼠伤寒沙门氏菌TA98和TA100在未加代谢活化系统时均显示出直接的致突变性；另外2种在加入代谢活化系统后也显示出间接的致突变作用。

Construction and Confirmation of Expressing Plasmids Carrying A Region of S.

基因重组鼠伤寒沙门氏菌防龋疫苗的研究——Ⅱ。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。