伤寒

To investigate the molecular diversity of the avian Salmonella, 12 S. typhimurium strains and 12 S. pullorum strains were identified by automatic microbiological assay and their genomic DNA were digested by restriction endonuclease Xba Ⅰ and typed by Pulsed-Field Gel Electrophoresis.

为应用脉冲场凝胶电泳从分子水平上对禽源沙门氏菌之间的差别进行分析和研究，本研究采用自动微生物鉴定仪对12株疑似鼠伤寒沙门氏菌和15株疑似鸡白痢沙门氏菌进行了鉴定。

Pullorum disease ; fowl typhoid ; blocking ELISA

鸡白痢；鸡伤寒；阻断ELISA ；单克隆抗体

To prevent cross-contamination of the virus from different animals, the feed eaten by the breeding hens cannot contain animal products; they cannot test positive for Salmonella enteritidis, Salmonella pullorum or Salmonella typhi, nor for avian flu or avian leukosis viruses; and they must also be free of external hormones and antibiotics.

魏逸之称呼这些是「clean eggs」──为了避免不同动物体内的病毒交互感染，种鸡所吃的饲料不得含有动物成分；它们体内不得检出肠炎沙门氏杆菌、鸡白痢沙门氏杆菌、伤寒沙门氏杆菌，以及A型禽流感、禽白血病等病毒，也不得有贺尔蒙与抗生素的残留。

Methods Seventy-four strains of Salmonella enterica serovar enteritidis (S. enteritidis), S. pullorum and S, gallinarum isolated from chickens were determined for biofilm using crystal violet staining, and the strain C050041 with well biofilm formation was chosen to construct a mutant library using transposon mutagenesis.

利用结晶紫染色定量法对74株鸡源的肠炎、鸡白痢和鸡伤寒沙门氏菌进行生物膜测定，选择生物膜生长较好的肠炎沙门氏菌C050041，采用转座子随机插入法构建突变株库。

From analysis of five misdiagnosed cases of typhoid fever,we have drawn the following causes for errors in thinking:thinking highly of the appearance while neglecting the essence:having blind faith in authority,thinking statically and in one-sided,excessively relying on accessory examinations,underrating the history-taking and being satisfied with the known diagnosis.

通过对伤寒误诊病例的分析，提出导致思维偏差的原因有：重视现象、忽略本质，迷信权威，静止和局限性的诊断思维，过分依赖辅助检查，轻视病史询问、满足已知诊断等。

We used the immunohistochemical ABC method to detect the expression of NF-κB at 2、 6、 12、 24、 48、 72 hours after infected by Salmonella typhimurium, and we deal the data statistically with univariant analysis of variance and Student-Newman-Keuls multiplerange test.

三组分别在感染鼠伤寒沙门氏菌后2、6、12、24、48、72小时6个时点，用免疫组织化学方法(抗NF-κB p65抗体、ABC法，石蜡包埋)和阳性细胞面积分布率半定量测定小肠、结肠及肾脏中NF-κB活性表达百分率，采用单变量多因素方差分析和Q检验做统计学处理。

The mutagen mechanism is presumed single factor dominant.(4)There was 85 percent transvertion in mutagen spectra, and they were all G:C→T:A transvertion. The maximal possibility of mutagen mechanism is analyzed as followed:ion beam implant → breakage cell surface and enter the interior → stimulate plentiful radical born → radical oxidize and damnify DNA → G becomes 8—oxoG → 8—oxoG pairing A → result in G:C→T:A transvertion(5)Production of transition was presumed that ion beam implantion damnified DNA, stimulate activity of MMR interveniented by methyl reducing.

诱发突变谱中碱基颠换比例是0.85，且是全部G：C→T：A颠换，推测低能离子束诱变鼠伤寒沙门氏菌得分子机制可能为：离子注入→通过破坏细胞表面进入内部→刺激产生大量自由基→自由基氧化损伤DNA→G变成8氧—7，8—二氢鸟嘌呤(8—oxoG)→8—oxoG与A配对→导致G：C→T：A颠换。

I said to the members of the Brahmo Samaj:'Suppose a typhoid patient is kept in a room where there are jars of pickles and pitchers of water. How can you expect the patient to recover?

我普经对梵志会的成员说：'假如把一位伤寒病人被关在放有大量泡菜和开水的房间里，怎么能期待病人能够恢复？

Typhimurium TA 98 and TA 100. 38 kinds of soy sauce showed significant direct-acting mutagenicity toward 2 strains without S9 mix; and other 2 kinds presented indirect-acting mutagpnicity with S9 mix.

亚硝酸盐浓度50 mmol/L时，38种酱油对鼠伤寒沙门氏菌TA98和TA100在未加代谢活化系统时均显示出直接的致突变性；另外2种在加入代谢活化系统后也显示出间接的致突变作用。

Blood in the stool caused by many reasons can be divided into two categories, one's own bowel disease, can be divided into:① intestinal ulcers and inflammation, such as bacterial and amebic dysentery sputum, typhoid fever, ulcerative colon Yandeng;⑧ parasitic infections, such as hookworm, schistosomiasis, etc.;② tumors, such as cancer, colon cancer, intestinal polyps;④ intestinal blood circulation disorder, such as hemorrhoids, intussusception, intestinal strangulation, etc.;⑥ injury, such as anal fissure, stool drying equipment inspection or scratches.

由很多原因造成的便血，可分为两大类，自己的肠道疾病，可分为：①肠道溃疡和炎症，如细菌性和阿米巴性痢疾痰，伤寒，溃疡性大肠炎等；⑧寄生虫感染，如钩虫，血吸虫病等；②肿瘤，如癌症，结肠癌，肠息肉；④肠道血液循环障碍，如痔疮，肠套叠，肠绞窄等；⑥损伤，如肛裂，大便干燥，设备检查或划痕。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。