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优球蛋白

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Euglobulin precipitation combined with gel filtration is a good method for purification IgM Mabs from ascites.

本文通过优球蛋白沉淀结合凝胶过滤法纯化腹水IgM型抗体,并与传统的硫酸铵沉淀结合凝胶过滤法相比较。

Methods:Ascites fluid was subjected to ammonium sulfate precipitation or euglobulin precipitation.

分别以硫酸铵沉淀或优球蛋白沉淀结合凝胶过滤法,从腹水中纯化IgM型单克隆抗体。

METHODS Using a combination of cryoglobulin precipitation,euglobulin precipitation, ion-exchange chromatography,(NH4)2SO4 precipitation and affinity chromatography, the impurity protein of different quality was removed, and the easy and effective condition was selected toincrease the purity and content of C2 in the final product.

方法利用冷球蛋白沉淀、优球蛋白沉淀、离子交换色谱、硫酸铵沉淀和亲和色谱几种方法,将血清中不同性质的杂蛋白除去,选择相对简单且分离效果好的条件,提高终产物中目标蛋白C2的纯度和含量。

Rabbit anti-thrombosis in oral fungus polysaccharides 18.5mg/kg, can significantly extend the specificity of thrombus and fibrin thrombus formation time and shorten the length of thrombus, reduce the thrombus wet weight and dry weight, reduce platelet count, lower platelet adhesion rate and blood viscosity, and can significantly shorten the guinea pig euglobulin lysis time, lower plasma fibrinogen levels, elevated plasmin activity, results showed that the fungus polysaccharides significantly antithrombotic effect.

抗血栓形成兔口服木耳多糖18.5mg/kg,可明显延长特异性血栓及纤维蛋白血栓的形成时间,缩短血栓长度,减轻血栓湿重和干重,减少血小板数,降低血小板粘附率和血液粘度,并可明显缩短豚鼠优球蛋白溶解时间,降低血浆纤维蛋白原含量,升高纤溶酶活性,结果表明,木耳多糖有明显的抗血栓作用。

Rabbit anti-thrombosis in oral fungus polysaccharides 18.5mg/kg, can significantly extend the specificity of thrombus and fibrin thrombus formation time and shorten the length of thrombus, reduce the thrombus wet weight and dry weight, reduce platelet count, lower platelet adhesion rateand blood viscosity, and can significantly shorten the guinea pig euglobulin lysis time, lower plasma fibrinogen levels, elevated plasmin activity, results showed that the fungus polysaccharides significantly antithrombotic effect.

抗血栓形成兔口服木耳多糖18.5mg/kg,可明显延长特异性血栓及纤维蛋白血栓的形成时间,缩短血栓长度,减轻血栓湿重和干重,减少血小板数,降低血小板粘附率和血液粘度,并可明显缩短豚鼠优球蛋白溶解时间,降低血浆纤维蛋白原含量,升高纤溶酶活性,结果表明,木耳多糖有明显的抗血栓作用。

Application of McAbs: the ascites of McAb A_12B_4 were purified with euglobulin precipitation, and tagged with colloidal golds, then the immuno-gold filtration assay was developed to examine avian influenza virus. The results showed that the positive outcome was gained with avian H_9, H_7 and H_5 influenza virus.

单抗的初步应用:A1284株腹水经优球蛋白沉淀法纯化后与胶体金标记,应用于胶体金试纸条检测试验,金标A1284株单抗与H9、H7、H5三种亚型禽流感病毒反应结果呈阳性。

This paper reporte dthe results of replacement of human Ciq with guinea pig Clq isolated by pseudoglobulin precipition method in solid phase enzyme-linked immuno-sobent assayfor the detection of human circulating immune complexes.

本文首次报道了以优球蛋白沉淀法提取的豚鼠C_1q替代人C_1q,作固相酶联免疫吸附试验检测人循环免疫复合物的结果。

Hemostatic mechanism total saponins of Yinfenglun were studied on plasma recalcified time, FT, KFTT and ELT. Result: TSY at different doses could markedly shorten bleeding time, reduce bleeding volume in mice. TSY also could shorten clotting time of mouse, rat and dog. TSY could influence both intrinsic coagulatian system and extrinsic coagulatian system, and had no effect of antifibrinolysis.

结果:荫风轮总苷不同剂量可明显缩短小鼠断尾出血时间,减少出血量,并能缩短小鼠、大鼠、犬凝血时间;可明显缩短家兔血浆复钙时间、凝血酶原时间和白陶土部分凝血活酶时间,但对大鼠优球蛋白溶解时间无明显影响,表明荫风轮总苷既能影响内源性凝血系统,又能影响外源性凝血系统。

The blood sample of carotid was drawn out in 24 rabbits 30 minutes after veinal injection of xinmaining and prothrombin time was recorded.

结果 :与对照组比较,不同浓度的心脉宁可延长凝血时间和凝血酶原时间,并缩短优球蛋白溶解时间。

The difference in the above indices between chalky and non chalky milled rice in Zhaiyeqing 8 was not remarkable. Crude protein content of chalky milled rice in Zhaiyeqing 8 was significantly lower than that of non-chalky rice, hot there was not remarkable changes between chalky and non chalky milled rice in Gangyou 527. Glutelin content fell significantly, albumin, globulin, prolamine and lysine contents did not change remarkably in chalky milled rice compared with non chalky milled rice in the two varieties.

窄叶青8号垩白米与非垩白米的上述各指标间无显著差异窄叶青8号垩白米的粗蛋白含量显著低于非垩白米,而冈优527垩白米的粗蛋白含量与非垩白米相比无显著变化2个品种垩白米的清蛋白、球蛋白、醇溶蛋白、赖氨酸含量与非垩白米间无显著差异,但谷蛋白含量显著低于非垩白米。

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Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

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