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The study suggest:(1) The observation of satellites in root tip and multiplex PCR makers could accurately identify the homozygous and the heterozygosis of 1BL/1RS translocation.(2) Through the restoring degree we could confirm the success of the transfer. The combination of the restoring degree and the agronomy property could increase the efficiency of practical application.(3) This study proposed the transformation from the physiological male sterile to the genetic male sterile, and then establish a method of utilize the strong superiority crosses.

其中复合引物PCR更适合于回交后代中大量目标种子的筛选;(2)恢复基因转育后代定株与不育系测交,依据测交恢复度可准确确定恢复基因在回交后代的延续,恢复基因定株定向转育与性状选择结合可大大提高转育后代的实际应用效果;(3)本研究提出的粘类小麦雄性不育恢复基因Rfv1定向转育体系,可有效地实现小麦由化控强优势组合向三系强优势组合的定向转化,即生理型不育途径向遗传型不育途径的定向转化,进而建拓一套杂交小麦强优势组合多途径利用新体系。

These genes are related to many plant functions, indicating that the heterosis was related with many metabolization pathways.1.2 The quantity of discrepant genes showed that the genetic discrepancy was notalways the same with the discrepancy of expressed genes, indicating that small-scale genetic discrepancy maybe result in large-scale genes discrepantly expression; in other way, some genetic discrepancies might not embodied on the discrepant genes in the experiment.1.3 Nine and eight discrepant genes were found respectively in the superior bulkand minor bulk in the dominant level.

1.2 各张芯片差异表达基因数量表明遗传差异与表达谱差异不尽一致,可能较小的遗传差异可以造成表达谱的巨大改变,而有些遗传变异可能没有在表达谱上表现出来。 1.3 从优势集团和负优势集团中分别找到9个和8个具显性表达水平的差异表达基因,研究表明显性表达水平的差异表达基因的聚合与杂种优势的表现没有相关性。

The higher percentage of cross-fertilized kernel specifically expressed pattern was observed in highly heterotic hybrid, after comparison of gene differential expression between cross-fertilized and self-fertilized kernels during the early development of seed in wheat and maize, respectively. This indicated that there may be have common molecular mechanism for heterosis in different crops.

本文对玉米和小麦在种子发育前期杂交和自交间基因差异表达进行了比较,发现杂交特异表达类型与杂种优势均存在一定的正向关系,说明不同作物杂种优势形成在分子水平上具有共同点,即相同表达模式的基因或基因群在不同作物杂种优势形成中发挥一致的作用。

isolates recovered from tuberculosis patients of rual morth Jiangsu province with variable-number tandem-repeatgenotyping, and analyze factors related to the dominant VNTR genotype.

目的运用可变数目串联重复分型方法研究农村结核病研究现场的结核分杆菌分离株的分子特征,分析与VNTR优势基因型相关的因素。

The RNAs synthesized in PK15 cells of GD and BF strain, representative strain of PCV2 dominant genotype (CHN-2H) and nondominant genotype (CHN-2A) respectively, were characterized by Northern blot and RT-PCR.

采用Northern blot和RT-PCR技术对PCV2优势基因型(CHN-2H)的代表毒株和非优势基因型(CHN-2A)的代表毒株进行了PK15细胞培养的基因转录分析。

The results showed:① The new gemplasms had high nutritive value and taste quality and were similar as the protogene type in each biochemical constitution.② The heterotic rates were negative orientation on the most of quality character,but it was evidently different from the growth vigour character.③ The external...

研究结果表明,(1)甜玉米新种质各种生化成分与原始基因型接近,营养价值高,食味品质好;(2)甜玉米品质性状优势率以负优势为主,明显不同于生长势性状的优势率;(3)普甜、甜脆、超甜玉米籽粒外观性状和糖分含量均受其各自的一对隐性基因控制

A combination of good genes and a stable environment has allowed this particularly ginormous fungus to continue its creeping existence over the past millennia.

优势基因的结合,再加上稳定的环境,使得这种巨型真菌可以在几千里保持活力。

Major difference of gene expression occurred in the qualitative level, especially cross-fertilized kernel specifically expressed pattern.

对三个不同优势的小麦杂交种子和自交种子及其正反杂交种与其双亲种子发育前期基因表达研究表明,杂交种子和自交种子之间存在明显的基因表达差异,且以质的特异表达为主;不同优势的正反杂交当代种子与亲本间基因表达发生了显著的改变,进一步分析发现单亲表达与杂种优势存在一定的正向关系,而杂种沉默表达与杂种优势存在一定的负向关系。

In maize, the results indicated that the patterns of gene expression altered obviously in cross-fertilized kernels as compared to self-fertilized kernels, major difference of gene expression occurred in the qualitative level. Further analysis indicated that higher percentage of total differentially expressed cDNA was observed in highly heterotic hybrid than in non-heterotic hybrid.

对四个不同优势的玉米杂交和自交在种子发育前期基因表达研究表明,玉米杂交种子与自交种子在发育前期存在明显的基因表达差异,强优势的杂交组合与弱优势杂交组合之间表现差异的总cDNA比例存在极显著差异,进一步分析发现,杂交特异表达与杂种优势存在一定的正向关系。

A frameshift mutation at base position 217 of exon 1 and a T/C missense mutation at base position 59 of exon 3 were found in Yingjing angora rabbit with a high frequency; a T/C same-sense mutation at base position 3 of exon 3 was found with similar frequency in three rabbit breeds.

在外显子1的217位检测到由TCT三碱基插入引起的移码突变,在外显子3的59位和3位分别发生了错义突变由T→C和同义突变由T→C。通过计算发现各位点不同的基因型和等位基因频率在3个兔品种中存在较大的差异,位点A、B在长毛兔和肉兔中均有各自的优势基因型和等位基因。

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The basic concept of FOP can be summarized as to further optimize effective prescription according to the standard of curative effects and with the aid of modern science and technology and theories of traditional Chinese medicine.

其基本内涵可概括为:以确有疗效的中药复方为研究对象,以现代科学技术和传统中医药理论为技术支持,以该复方所治病证的药效响应为评价标准,以优化重组疗效更优的新复方为研究目的。

Ever since our world has been a world, native forests have been indiscriminately exploited by man.

自从我们的世界一直是世界原生森林被任意剥削人。

I don't… don't know. He's unconscious.

我不……我不知道他休克了。