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Gondii, were cloned by PCR respectively. The PCR products were digested by the corresponding enzymes and ligatd into the intermedial vectors. Finally, the inducible RNAi vector, pBSK-HSP70/5UTR-IntronC-HSP70/3UTR, with the HSP70 gene promotor as a promotor, the intron C sequence ofβ-tubulin gene as intervening sequence, 3UTR sequences of HSP70 gene as transcription stop signals, was constructed successfully, and the results of sequencing were correct. 3The construction of the inherited and inducible RNAi vector system of T. gondii: The fragment of SAG1/5UTR-eGFP-SAG1/3UTR in pBSK-SAG1/GFP vector was cloned into the vector of pBSK-HSP70/5UTR-IntronC-HSP70/3UTR to construct pBSK-GFP-Hairpin vector, then the fragment of GFP-Hairpin in pBSK-GFP-Hairpin vector was cloned into pHANA-0.5 vector.

弓形虫可诱导的反向重复序列RNAi载体的构建:设计引物,通过PCR分别扩增弓形虫HSP70基因5&UTR启动子序列(HSP70/5UTR)、HSP70基因3&UTR序列(HSP70/3UTR)及β-微管蛋白基因内含子C序列,通过酶切连接,构建以弓形虫热休克蛋白HSP70基因启动子进行驱动的,以β-微管蛋白基因内含子C序列作为间隔序列,以HSP70基因3UTR序列作为转录终止信号的反向重复序列RNAi载体pBSK-HSP70/5UTR-IntronC-HSP70/3UTR,序列测定结果正确。

Objective: To investigate the protective effect ofα-melanocyte stimulating hormoneon ARDS rats with acute hemorrhagic shock and sequentially administered endotoxin intratracheally and its mechanism.

目的:研究α-黑素细胞刺激素对急性失血性休克肺内毒素损伤两次打击ARDS模型大鼠的保护作用及其机制。

Hypertonic electrolyte glucose solution group, isosmotic electrolyte glucose solution group and control group.

目的 比较应用1.8%的高渗氯化钠葡萄糖溶液和0.9%的等渗氯化钠葡萄糖溶液进行烧伤早期抗休克治疗对补液量及肺血管通透性的影响。

Objective To compare the effects of hypertonic electrolyte glucose solution with isosmotic electrolyte glucose solution as different resuscitation fluids on volume of fluid replacement and pulmonary vasopermeability at early stage of burn shock.

作者:沈小鹏,胡森,柴家科,邹晓防,高瑗目的比较应用1.8%的高渗氯化钠葡萄糖溶液和0.9%的等渗氯化钠葡萄糖溶液进行烧伤早期抗休克治疗对补液量及肺血管通透性的影响。

Sequences analysis indicated 26 unique cDNA sequences that encoded putative proteins showing similarities to HL35 antigen U, hlim3, heat shock protein, NADH dehydrogenase subunit 1 and calreticulin of H. longicornis, disulfide isomerase of Ambiyomma variegatum and Ixodes scapularis.

结果表明:在长角血蜱雌蜱唾液腺cDNA表达文库中获得26个长角血蜱免疫相关cDNA序列,其编码蛋白与长角血蜱HL35、黏附素hlim3假定蛋白、热休克蛋白、NADH脱氢酶、钙网蛋白以及杂色花蜱和肩突硬蜱的二硫异构酶等具有同源性。

Knurled shock collars allow you to get a good grip for easy adjustments and o-rings in the collars prevent any unwanted movement.

技术特性休克衣领,让您获得良好的抓地力,方便调整和O型环,在衣领,防止任何不想要的运动。

Results: Twenty one differential protein spots were screened and 5 differential proteins were identified. They included Ezrin protein, transcription elongation factor A, and lamin A/C which were up-regulated in LoVo cells and non-metastatic protein expressed in isoform a (NM23 A) and heat shock protein 8 (HSPA8) which were up-regulated in SW480 cells.

结果:筛选出21个差异蛋白质点,成功鉴定出5种差异蛋白质,其中在LoVo细胞株中表达上调的蛋白有埃兹蛋白、转录延长因子A (transcription elongation factor A)和甘氨酸A/C,在SW480细胞株中表达上调的蛋白有NM23蛋白[non-metastatic cell 1 protein (NM23A) expressed in isoform a]和(heat shockprotein 8,热休克蛋白8)。

The main results and conclusions are as followings.1, The histone deacetylase inhibitors were used to feed the larve of the fly, and then the polytene chromosomes were observed under the microscope.

得到的主要结果和结论如下: 1,通过用去乙酰化酶抑制剂处理果蝇幼虫,观察果蝇多线染色体在热休克基因处的形态变化,发现去乙酰化酶抑制剂介导的乙酰化水平的升高可引起染色体结构的显著变化,推测乙酰化修饰不仅影响分子间的相互作用而且还可以影响染色体的高级结构。

Center of war wound and trauma care in plateau,general hospital of Tibet military area command,lasa 850003

目的 探讨高原(海拔>3658m)不同人群创伤失血性休克患者综合治疗的特点。

In cases of rupture, lightheadedness or shock may occur.

输卵管破裂是会出现晕厥或者休克

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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。