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DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.

诱导出的DC共同表达CD83和CD14分子,CD83分子表达明显高于国内报道;肝癌患者DC培养上清中TNF水平高于健康人,提示肝癌患者DC仍具一定的抗原呈递能力,适当调控可使其行使APC功能,以期在肝癌个体化疫苗中发挥作用;DC负载肝癌可溶性抗原后,抗原呈递能力降低,BCG HSP70可促进DC成熟,增加其抗原呈递能力,预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子;肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞,活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主,同时分泌较高水平Th1型细胞因子IL-12和TNF,并抑制4种肝癌细胞生长;冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤,提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用;用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽,在体外诱导肝癌特异性淋巴细胞,活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF,并具较强杀伤活性,此CTL同时具备NK细胞特征;DC对肿瘤细胞的抑制作用可能是通过吞噬实现的,Fas-L在DC抑制中也起一定作用;DC对人肝癌裸鼠皮下移植瘤的抑制率为97%;在肝癌DC疫苗的作用中,至少联合3种以上机制,即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。

Numerically evaluate the value of the largest Lyapunov exponent on diagnosing the various stenosis degrees of internal carotid arteries in white rabbits and in order to diagnose the angiostenosis with transcranial Doppler ultrasound.

探讨了血流信号的最大Lyapunov指数在诊断白兔颈内动脉不同程度狭窄中的价值,以期达到利用经颅多普勒超声量化诊断血管狭窄的目的。

To explore the properties of the cortical response to visual coherent motion and reveal the possible neural mechanism of cortical deficit of visual motion perception in anisometropic amblyopia so that we can diagnosis and treat it earlier and specifically.

以期能进一步揭示屈光参差性弱视视觉运动功能缺陷的可能皮层机制,为早期发现和早期治疗潜在的弱视病人提供理论依据并帮助临床根据弱视不同的脑功能区损害特点进行有针对性的诊疗。材料与方法1。

Therefore,the experiment of clearance rate and filter rate of Anodonta woodiana was done in the lab to verify its effectiveness.

故以本论文於研究室中进行圆蚌的藻类滤除试验,以期在未来田间试验放养圆蚌中能够有所依据,达到最经济的且有效的处理效果。

On the basis of ESTs, three antenna specific protein—Ac-ASP1, Ac-ASP2 and Ac-ASP3, were cloned and identified through the transcriptional profiles and immunocytochemical localization in antennal sensilla. All above will produce an important basis to the exquisite olfaction and action of discrimination against others and resistance to mites of A.

在此基础上,着重通过对中蜂主要的触角特异蛋白——Ac-ASP1,Ac-ASP2和Ac-ASP3进行基因克隆,并通过其在触角中的转录表达谱和在触角不同感器内的亚细胞定位,以期对上述分子进行鉴定,最终对揭示中蜂灵敏的嗅觉以及独特的排异和抗螨能力奠定重要的基础。

To investigate the antioxidant activities and total polyphenol content in two Zicao species, Arnebia euchroma and Lithospermum erythrorhizon.

背景/目的本研究拟探讨新疆紫草及紫草之抗氧化活性及其总多酚类成分之含量,以期了解两者间之药理活性差异。

Objective: To explore the relationship between angioarchitectures of cerebral arteriovenous malformations and hemorrhage and to reveal the risks of bleeding.

目的:探讨脑动静脉畸形的相关血管构筑学因素与出血的关系,以期预测脑AVM出血的危险性。

The COD value in the dorsal and dorsolateral subdivisions of the PAG in the kindled audiogenic seizures-prone rats were significantly higher than that in the naive audiogenc seizures-prone rats.

用光镜观察PAG各节段各亚区切片上突触密度的变化,以期在光镜水平上了解PAG在惊厥状态下超微结构的某些变化。

Whereas, this article will adopt the autology of New Media Art to discuss its meanings of ontology. The newness of New Media Art will be highlighted. That is, how we orientate and interpret the core of"new", how we distinguish and classify the impact caused by the application of new technologies and mixed media, how to explain and analyse the contradictive as well as unitive relations among image, time and space in New Media Art. This article argues about some controversial academic notions and defines aesthetic scopes logically, in a hope to provide the study of New Media Art with tangible aims and stands.

有鉴于此,本文从媒体艺术的媒体属性入手,对新媒体艺术的本体论意义进行阐释,特别突出了新媒体艺术&新&的本质(即我们怎样定位和解读其&新的内核&);对现代技术和混合媒体的应用给当代艺术带来的影响与冲击进行甄别与归类;对新媒体艺术中的影像、时间与空间因素之间内在的既矛盾又统一的复杂关系加以分析,以期对争议已久的学术概念和美学范畴进行合理的界定,为我们对新媒体艺术发展趋势的研究与实践确立明确的目标和立足点。

According to research progress of Phytophthora root rot, this paper briefly presented the progress on the defense genes of soybean, avirulence gene of Phytophthora sojae and inter-action of soybean-Phytophthora sojae.

根据大豆抗疫霉根腐病的分子机制的研究进展,文章阐述了大豆防卫基因、大豆疫霉菌无毒基因以及大豆-疫霉菌互作的研究进展,以期为抗病品种的选育提供理论基础。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力