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Compared with L7 cells not induced by Dox, 7 genes were upregulated and 12 genes were downregulated at Dox 6μg/ml, of which 4 were selected for RT-PCR to confirm the result from cDNA array, expression of four genes were consistent with that from cDNA array.

选择含有265个转移相关基因的cDNA微阵列膜,以Dox不诱导为对照,检测在Dox6μg/ml诱导下,L7细胞中差异表达的转移相关基因。

Using the principle and method of matrix transformation, the matrix model of VRPTW is developed.(3) Through analyzing the time windows and distance matrix of the nodes, we construct a probability transferring matrix, which is used to produce the initial scheme of VRPTW.

以有时间窗的车辆路径问题的矩阵模型为基础,对客户点的时间窗和距离矩阵进行分析,构造了有时间窗的车辆路径问题的概率转移矩阵,并利用基于概率转移矩阵的初始化方案生成方法,产生有时间窗的车辆路径问题的初始化方案。

The catalysts were prepared by impregnation and precipitation method, and the active carbon,γ-Al2O3, silica and kieselguhr were selected as the carriers of catalyst. the effects of reaction conditions, such as reaction temperature, types of solvents, and types of hydrogen donors were investigated, It was found that the Pd/C catalyst was the best one, and its proper reaction conditions were also obtained as follow: sodium formate as hydrogen donor, water as solvent, reaction temperature of 70℃ and reaction time of 2.5h.

以活性炭、γ-Al2O3、硅胶和硅藻土等为载体,分别采用浸渍法和沉淀法制备了催化剂,对于反应温度、溶剂及氢供体的种类等反应条件的影响进行了研究,发现以沉淀法制备的Pd/C催化剂是对转移加氢最有效的催化剂,初步确定了适宜的反应条件为以甲酸钠作氢供体、水作溶剂及70℃反应2.5h等。

Buck and Boost topology switching power conversion circuits as the core of this system, ultra low power MSP430F1222 microcontroller-based controller, microcontroller produced by the PWM pulse width modulation signals, respectively, two-way control switch voltage conversion circuit the turn-on and turn-off in order to achieve the above two kinds of topology switching power conversion circuit, and then to draw power from the flow of electrical energy has been filled into the rechargeable battery, and using microcontroller A / D input voltage implementation of the monitoring, according to the input voltage switching buck - circuit at a lower cost to achieve a highly efficient energy transfer, and the circuit is simple, the parameter to determine easy, self-low power consumption,to achieve full-featured.

本系统以Buck降压和Boost升压拓扑结构开关电源变换电路为核心,以超低功耗单片机MSP430F1222为主控器,由单片机产生PWM脉宽调制信号,分别控制两路电压变换电路中开关管的导通与关断从而实现以上两种拓扑结构开关电源变换电路,进而从一直流电源中吸取电能充入可充电电池,并利用单片机A/D对输入电压实施监测,根据输入电压切换升降压电路,以较低的成本实现了高效率的电能转移,并且电路简单、参数确定容易,自身功耗低,实现功能完整。

Based on these previous work, in this research, in order to explore the the molecular mechanism of enhanced tumor cell immunogenicity treated by elemene and mytomycin C compositely, we used murine Hca-F ascitic hepatic carcinoma ( high lymphatic metastasis substrain of H22) as an experimental model to compare the immunoprotective effects on Hca-F cells as well as the mechanism of the effects caused by HSP70-peptide complex purified from untreated tumor cells, EC-TCV and BCG; We studied the mechanism of increased tumor cell membrane HSP expression treated with elemene by the techniques of RT-PCR and immunoelectron microscopy; In order to deeply elucidate the antitumor effects and the molecular mechanism of elemene in increasing tumor cell immunogenicity we analyzed and compared the effects of elemene and heat shock on the gene expressions of tumor cell using gene expression profile chip.

本论文在上述研究成果的基础上,选择小鼠肝癌H22的高淋巴道转移亚系Hca-F为模型,进一步比较了未处理肿瘤细胞来源、榄香烯复合瘤苗来源的和卡介苗来源的HSP70-肽复合物对Hca-F的免疫保护效应、效应机制,探讨榄香烯-丝裂霉素复合处理增强肿瘤细胞免疫原性的分子机制;以Hca-F和人肝癌细胞株HepG〓等为模型研究了榄香烯处理增强肿瘤细胞膜热休克蛋白表达的机制;以HepG〓为模型用表达谱基因芯片研究比较了榄香烯与热休克处理对肿瘤细胞基因表达的影响,从而进一步阐明榄香烯抗肿瘤作用及增强肿瘤细胞免疫原性的分子机制。

In the absence of organic solvent and phase-transfer reagent, the catalytic oxidation of cyclo- hexene to adipic acid with aqueous 30% hydrogen peroxide can be achieved in the presence of tungstic acid/acidic organic additive.

以钨酸/有机酸性添加剂为催化体系,在无有机溶剂、相转移剂的情况下,催化 30%过氧化氢氧化环己烯合成己二酸。当钨酸∶有机酸性添加剂∶环己烯∶过氧化氢=1∶1∶40∶176(摩尔比,钨酸用量为 2.5 mmol)时,使用有机酸性添加剂考察钨酸的催化性能,结果表明以钨酸/间苯二酚催化氧化环己烯的催化效果最优,反应 8 h 时己二酸分离产率达 90.9%、纯度为~100%;而不使用有机酸性添加剂时,己二酸分离产率只有 72.1%,产品纯度为 96.2%。

The field survey, sampling, testing and collection of the geological and hydrogeological data were done for the description of evolutional geological structure and sedimentation and division and definition of water-bearing system and hydrogeological age and hydro-pressure system and study of the vadose field and chemical field. By establishing the rammer numerical model, the sandy mudstone thickness recovery model, water-rock interaction particle translative simulation and hydrogeochemical simulation, and using geological statistics, analogism and complex analysis method, water circulation and water exchange of each layer in each study stage have been researched, The results show the regularity of the vadose field evolution in different stages.

本项以野外调查、采样测试和搜集的地质、水文地质大量第一性资料为依据,在论述盆地的地质构造演化、沉积演化的基础上,以含水系统、水文地质期和水压系统类型的划分和定位为研究的切入点,渗流场、化学场为研究主线,通过建立压实数学模型、砂泥岩恢复厚度数学模型、水岩作用物质成分转移的实验模拟、水文地球化学模拟、地质统计法、类比法、综合分析法等方法,模拟计算了各研究层在各研究时期水循环特点和水交替强度,揭示了各研究层渗流场在地史进程中形成演化的规律性。

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The invention adopts halogen acetylized crosslinked polystyrene resin and hydroxybenzyl alcohol as the raw material, and the solid phase synthesis carrier with hydroxymethyl as the active group is synthesized through the joint catalysis of N, N-dimethyl formamide used as the reaction solvent, inorganic base and phase-transfer catalyst.

本发明采用卤乙酰化交联聚苯乙烯树脂和对羟基苯甲醇为原料,在以N,N-二甲基甲酰胺为反应溶剂、无机碱及相转移催化剂的共同催化下合成了一种以羟甲基为活性基团的固相合成载体。

The fifth chapter examines how the Research Clique, an intellectual group closely related to the Progressive Party and the constitutionalists, perceived revolution by studying their journal Jiefang yu gaizao (The Emancipation and Reconstruction Semi-Monthly).

职是之故,论文共分八章:第一章交代方法;第二章绘测五四知识场及其组成;第三章以五四前期掀起新文化巨浪的《新青年Ⅰ》为对象,理解当时最活跃的新文化份子在经历建立共和及其后乱象后,到底是如何延续辛亥的革命思考;第四章分析国民党群体的《建设》、《星期评论》,以明了他们在三民主义旗帜下的革命观;第五章以研究系的《解放与改造》为分析对象,看这群与进步党或立宪派渊源颇深的知识群体,又是如何理解革命的;第六章则把视线转移至自由派知识份子的《努力周报》,看这群受英美自由思潮、实验主义影响的群体怎样在细部改良的思想驱动下与革命思潮相抗;第七章则分析热烈拥抱革命的马克主义群体的《新青年Ⅱ》、《向导》,以期还原他们的革命理论;第八章则综合此前所得,挑明五四的革命论理形态大致可分为静态革命观与能动革命观两类。

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