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Objective Establishment of a hybridoma cell line secreting a monoclonal antibody to facilitate iˉdentification of human tissue kallikrein(KLK1gene,hK1protein).Methods Mice were immunized with E.coli-exˉpressed GST-hK1fusion protein and their spleen cells were fused with SP2/0myeloblastoma cells.

目的 将人组织激肽释放酶(基因命名为KLK1,酶命名为hK1)cDNA克隆入大肠杆菌表达质粒,以重组菌表达的GST-hK1融合蛋白免疫小鼠,获得了分泌hK1特异单克隆抗体的杂交瘤细胞株。

The purpose of this thesis is to explore other yet undiscovered contrast origins and unused contrast agents of third-harmonic-generation inside biological tissues, and apply to the preliminary studies of medicine based on a nonlinear laser scanning microscopy system using a femtosecond Cr:forsterite laser centered at 1230-nm.

这篇论文的目的是利用了以中心波长为1230nm的铬贵橄榄石雷射做为光源的非线性雷射扫瞄显微镜系统去探索生物体中其他仍未被发现的三倍频对比来源以及尚未被使用的三倍频外加显影剂,并初步的应用於医学研究上。

Objective: To observe the toxicity and therapeutic efficacy of Litsea Cubeba oil treating experimental mouse infected by Cryptococcus neoformans with intragastric administration, which is evaluated by the internationally accepted criteria, including survival rate and clonal formation unit count in lung and kidney, to evaluated the feasibility of clinical use and provide initial evidence for clinical use of Litsea Cubeba oil.

目的:以小鼠的生存率及肾脏、肺脏菌落形成单位计数为指标,评价山苍子油灌胃治疗实验性小鼠系统性新生隐球菌感染的疗效,从而为山苍子油的临床应用提供实验依据。

Objective To observe the blood supply of cutaneous nerves in the hand for design of the cutaneous neurovascular island flaps.

目的 观测手背皮神经血液供应情况,为以皮神经及其营养血管为蒂的岛状皮瓣的设计提供形态学依据。

1Cr5Mo, 12CrlMoV and 20# are three types of pearlitic heat resistant steel and widely used for high temperature tubes in petro-chemical industry, so their safety assessments are necessary for the sake of operation safety in high temperature cond.

以1Cr5Mo、12Cr1MoV和20钢三种常用作石化装置高温炉管材料的珠光体耐热钢为研究对象,针对其在高温服役中的安全评估问题,在以往工作的基础上,采用数据库和面向对象编程技术研究开发了一套高温管线安全评估决策支持系统,其目的是为大连石化公司数十套装置中加热炉辐射段管线与这三种材料相关的安全评估提供决策支持。

The paper aimed at the burning spent gases and calcium carbonate nanoparticles can be successfully synthesized by wet-chemical segregative decarbonization technique, while another environmentally conscious materials- oxynitride ceramics Sialon etc.

本文以燃烧烟气为对象,采用湿式固碳分离技术合成纳米碳酸钙,处理后的氮气制备氧氮化物环境友好陶瓷,达到抑制碳排放和资源增效多重目的,为挑战该世界技术难题奠定了扎实的基础并提出展望。

In this study, two Sonification schemes, Tx2Ms and Im2Ms, are proposed to extract the melody between the lines, i.e., both the lines in verses and the lines in strokes.

本研究分别针对五言绝句与草书提出两种可听化的方法,目的在於萃取出诗词字里行间的韵律之美以及书法挥毫纸上的线条之美,将其映射为数字化资料,并以演算法作曲化数字为旋律。

Objective To use distinctive water-medicine cup therapy for treatment of cervical spondylopathy of cervical type as a sample to provide a more effective cupping method for clinic.

目的:以特色水药罐治疗颈型颈椎病为例,为临床提供一种更有效的拔罐方法。

objectiveto evaluate and explore the reconstruction technique of straddling region reversal flow axial composite auricular island flap with blood-supply from supraorbital artery or supratrochlear artery for repairing large alar defects.methodsfrom may of 2004 to macth of 2008, 6 patients with the lager alar defects were studied.

目的:探讨以眶上动脉或滑车上动脉为供血源的跨区反流轴型耳廓复合组织岛状瓣,修复大面积鼻翼缺损的临床应用特点。方法:从2004年5月~2008年3月间,我科选用游离眶上动脉或滑车上动脉与颞浅动脉额支的吻合支为供血源所营养耳廓复合组织瓣,经皮下隧道转移至鼻翼缺损区,ⅰ期修复6例大面积的鼻翼缺损的患者。

Concerning her entering Tibet, there are two-party causes: the mighty of the Tang dynasty and Tangtaizong's open national policy; Songzanganbu's founding the Tubo kingdom and the admiration and naturalisation to the politics、economy and culture of the advanced nationalites.

和亲一词自西汉开始演变为中原汉族下嫁公主予少数民族王国的君长,这种和亲政策为历代汉族统治阶级所采用,以达到各自不同的统治目的。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。