以...为目的

In our experiment, the GADes gene from rat brain was cloned and expressed in E.coli, so as to make the recombinant GADes produced by gene technology for clinical application and further study on the relation between GAD and type 1 diabetes mellitus.Firstly, with the pUClS/GADes recombinant plasmid as template, the full-length encoding sequence of GADes was amplified by PCR, and cloned into pGEM-T vector. Secondly, the target gene was cut by EcoRI and inserted into restriction sites of pET42a vector for expression.

本研究首先用PCR方法，以pUC18/GAD_(65)为模板，扩增目的基因，克隆到pGEM-T载体中，构建pGEM-T/GAD_(65)重组质粒，然后用EcoRI酶切，切下的目的基因片段插入pET42a融合型表达载体中，经酶切鉴定和测序，证实插入方向、读码框架正确；重组表达质粒转化大肠杆菌BL21(DE_3)中诱导表达，表达产物经亲和层析纯化后获得了融合蛋白GST-GAD_(65)。

The direct source of this theory originated from the research made by J. S. Mill and G. Frege about the relation between reference and meaning. For the purpose of resolving philosophic problems like "existence", Russell defined the word "description" as propositional function with accurate meaning and used it as a weapon to serve his philosophy.

它直接渊源于穆勒和弗雷格的对指称与涵义的关系研究；罗素从解决"存在"等哲学问题的目的出发，将摹状词在命题中化解为具有精确涵义的命题函项，并以之为武器为他的哲学服务。

Part Two: 3-D Finite Element Method Study on Maxillary Protraction Objective: To explore the change rule of stress、 strain and displacement of craniofacial complex against different strength and direction of maxillary protraction, so as to provide the scientific basis for the selection of force system in clinical treatment.

第二部分上颌前牵引的三维有限元研究目的：探讨不同大小和方向的前牵引力对整个颅面复合体的应力、应变和位移变化规律，以达到为临床力系统的选择提供科学依据的目的。

Hence this study purposed on the relationship between strike prices and premium through empirical research and try to find a strategy of arbitrage on spread of market anomaly.

所以本文冀希将实务研究导入选择权交易中的各个履约价和其相关权利金间是否存在一必然关系性来做为研究目的，也冀希从研究中找寻一可行性之套利策略以探求市场异质性之价格误谬进行套利目的。

The research is based on structural formula questionnaire survey and material collection method.

为达成研究目的，系以结构式问卷调查为资料搜集方法，自行发展编制「专业伦理思考问卷」做为研究工具，共发放约550份研究问卷，回收有效问卷计有337份。

The Essay is focused on the new election system of Japanese House of Representatives' to find out the roles of election system in Japan democratic Progress. To perfect the electoral system, Japan had mended its election system of the House of Representatives 14 times since 1889 till 1994. In 1993, some political issues, included the reform of election system, eventually led Prime Minister Miyazawa Kiichi to resign and ended the Liberal Democratic Party' 38-year-old reign, as well as the so-call"55 system（the party system of 1955）".During the past century, Japan has tried small, medium and large sized district systems, currently "First past the post with Proportional Representation System", to constitute a fair and better system which is expected to improve Japan's political environments and make voters trust the politics.

中文摘要本论文系以日本众议院新选举制度为研究之核心，探讨日本在其民主化过程中，选举制度所扮演之角色，日本为让选举制度更臻完善，自一八八九年起至一九九四年止，共计针对其众议院选举制度进行了十四次之修改，甚至於一九九三年，因选举制度等政治议题之改革，导致自由民主党之宫泽喜一内阁下台，结束该党长达三十八年之长期执政，日本政治上所谓「五五体制」亦至此宣告结束，过去百余年来日本先后实施过小选举区制、中选举区制、大选举区制及目前实施之小选举区比例代表并立制，其主要目的系为建构一套更公正、公平、优质之选举制度，让其政治更清明，让选民对政治更有信心。

Objective:to study the epidemiologic characteristic of mortality of malignant tumors in zhongshan city.method:selecting the investigated datum of mortality of malignant tumor in zhongshan city as sample uses sas statistics software to analyse and forecast the mortality by time series.results:morlality of male malignant tumor and both male and female malignant tumour showed the risen trend from 1970 to 1989.the trend will be kept in future.both will be separately risen to 121.05/105 and 83.92/105 in 2 000.mortality of female malignant tumour will be kept in about 43.59/105 in 2000.couclusion:mortality of both male and female malignant tumor takes on the trend of diffirent change.the male showed risen trend;the female showed the fluctuation of non-trend random.rising mortality of malignant tumor greatly threaden the crowd health.

目的：研究中山市恶性肿瘤死亡率的流行学特点，为制定防治计划提供依据。方法：以中山市恶性肿瘤死亡率调查资料为样本，应用sas统计分析软件对恶性肿瘤死亡率进行时间序列分析与预测。结果：1970～1989年男性恶性肿瘤死亡率和男女合计恶性肿瘤死亡率均呈现上升趋势且有继续保持的趋势。到2000年将分别上升为121.05/10万和83.92/10万左右；女性恶性肿瘤死亡率呈现无趋势的随机波动且将继续保持下去，到2000年保持在43.59/10万左右。结论：中山市男女恶性肿瘤死亡率呈不同变化趋势，男性恶性肿瘤死亡率呈现上升的趋势，女性恶性肿瘤死亡率呈现无趋势的随机波动。恶性肿瘤死亡率的上升对人群的健康构成重大威胁。

objective to study the correlation among psychosomatic health factors,depression,anxiety and sleep status in college students,to analyze influence factors in sleep status.methods with questionnaire opened in-vestigation,200college students were evaluated using cornell medical index,self-rating depression,self-rating anxiety scaleand pittsburgh sleep quality index.stepwise regression analysis was used.re-sults total score of sds was35.55±7.8,and sas was0.48±0.9.each score of cmi was higher than normal range,the highest was digestion system,next was respiration system,then,fatigue,eyes and ears,anxiety,sensitivity,tension,maladjustment in turn.total average score of psqi was6.32±3.6,ordinary sleep were115cases（57.5%）,good and bad sleep were42（21%）and43（21.5%）cases respectively.influence factors in total score of psqi were sas,cmi,respiration system,anxiety,past healthy,digestion system,fatigue,sensitivity,sds and malad-justment in turn,using stepwise regression analysis,total score of psqi as dependent variable,each factor score of cmi,total score of sds and sas as independent variables.conclusion problems of psychosomatic health,anxiety and depression could both lead to sleep disorder.

目的 研究在校大学生心身健康因素及抑郁焦虑等与睡眠状况的相关性，并对影响睡眠状况的有关因素进行分析。方法采用问卷式的开放性研究，对在校的200名大学生进行康奈尔心身健康问卷、自评抑郁量表、焦虑自评量表和匹兹堡睡眠质量量表的测定，采用逐步回归分析方法。结果 sds总分为35.55±7.8、sas总分为0.48±0.9，cmi评分：消化系统最为严重评分为2.52，其次为呼吸系统、疲劳感、眼和耳、焦虑、敏感、紧张、不适应等，评分均高于正常常模。psqi总均分为6.32±3.6，一般睡眠有115例占57.5%，睡眠质量较好42例占21.0%，睡眠质量较差43例占21.5%。评估对睡眠状况的影响程度，以psqi总分为因变量，选择cmi各因子分和sds、sas总分作为自变量，进行多因素逐步回归分析，进入方程的因素依据标准化偏回归系数，影响psqi总分的因素依次为sas总分、cmi总分、呼吸系统、焦虑、既往健康、消化系统、疲劳感、敏感、sds总分、不适应（f=226.8；p.01；r=0.73）。结论心身健康问题和焦虑抑郁可导致睡眠障碍。

So to analyze in the aspect of description, it is useful to delouse the fount how inarticulateness comes into being; Based on the economic relationship and from the viewpoint of the pursuit of aim, the object of studying, the original creation of aesthetics, as well as the articulate knowledge and inarticulate knowledge, Polanyi distinguished the acquisitive way of science and technology. The development model of personal Knowledge can be generalized as the enthusiasm of seeking knowledge; Polanyi revealed how personal factors of mathematician cut across logical chasm, and gained mathematics findings in math" s activities. Thus he proved mathematics was also activity-needed techniques as natural science; Last evaluating the theory of "Personal Knowledge systematically, it can reveal its realistic significance and limitation.

波兰尼把认知结构区分为可言传知识与不可言传知识，与20世纪以来西方哲学的中心课题转向语言学的研究一致，所以从言述的角度出发，有助于了解不可言传性如何形成的根源；波兰尼以经济关系为立足点，从科学与技术的目的追求、研究对象、美的原创性，以及从可言传知识与默会知识的角度对两者的获取途径做了区分；其个人知识发展模式可以概括为：求知热情→启发性热情→说服性热情……；波兰尼揭示出数学家的个人因素是如何在数学活动中跨越逻辑鸿沟，取得数学发现的，从而证明数学同自然科学一样，也是一项需要技能的活动；最后对&个人知识&理论作一系统的评价，揭示&个人知识&理论的现实意义和局限性。

Objective This paper will regular IT profession and medical treatment profession research and application, and complete medical treatment health sector planning 80% hospital realization information manage eyemark, truly realization hospital with patient as central comprehensive information management "digitizing hospital"pass Computer-based Patient Record the General Card system research.

目的通过电子病历一卡通系统的研究，指导IT行业和医疗行业的研发与应用，完成医疗卫生行业规划8%医院目的通过电子病历一卡通系统的研究，指导IT行业和医疗行业的研发与应用，完成医疗卫生行业规划80%医院实现信息化管理的目标，真正实现医院以病人为中心的全面信息管理的&数字化医院&。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。