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We amied the BCP sequence and the preS2 gene sequence of HBV separately as the target region, according to HBV DNA sequence of Chinese strain, synthesized the set of specific primers, amplified the sequence by PCR method from the serum of patients with CHI, verified by restriction analysis, subcloned into pGEM Teasy vectors, employed polyacrylamide gel electrophoresis to display the deletion mutation and selected the clones with differential length to be sequenced.

本研究应用PCR技术及其他分子生物学技术,分别以HBV转录调控序列BCP以及表面蛋白编码序列pre S2为靶基因,以中国株HBV基因序列为依据,设计特异性多聚酶链反应引物,分别自43例、51例慢性HBV感染患者血清中扩增出目的片段,构建质粒Teasy-BCP和Teasy-pre S2,酶切鉴定后,采用聚丙烯酰胺凝胶电泳技术展示缺失突变,再经DNA测序以了解在慢性乙型肝炎患者体内的病毒变异情况。

And this theory holds the view that any translatorial action is purposeful, In order to achieve the purpose, the translator should be creative and decisive to produce the "translatum" of the target culture.

目的论认为任何翻译行为都是有目的的行为,为实现翻译目的,译者应充分发挥其创造性,根据目的文本的功能,对原文进行操作,以产生符合目的语的文本。

The community feature university education and relevant industries, regard resource-sharing of school district as main purpose, logistic industry of the school socializes will turn a goal afterwards.

我国大学城的形式主要是由若干所大学在同一区域内聚集,形成一个诸高校相对集中的、以大学教育和相关产业为特色、以校区资源共享为主要目的、以后勤产业的社会化为目标的城市社区。

OBJECTIVE : to analyse the replication activity of NS5B proteins of HCV genotype 1a and 1b when use 3'terminuses of negative RNA strand from HCV 1a, 1b and 2a as templates by performing RdRp assay in vitro and offer some useful data to optimize the model for the screening of anti-HCV drugs targeting at NS5B.

目的:通过体外的RNA依赖的RNA聚合反应,分别比较HCV 1a和1b型来源的NS5B蛋白以不同HCV基因型负链3'末端RNA为模板时的复制活性,为优化以NS5B为靶点的抗HCV药物筛选模型提供理论依据。

Analyzing in a general view and studying systematically guide theories, basic processes and means of figure re-creation in literature interpretation, the thesis illustrates with the Chinese rendering A Storey Of Building written by Jia Yimu, and demonstrates that a rendering with both social and aesthetic values should proceed from thought exchange, basis on the practice of acceptants, aim at interpreting of artistic re-creation and dedicate to the national cultures and cultural exchange.

论文从宏观角度出发,以甲乙木汉译本《一层楼》为范例,试图通过对文学翻译中艺术形象再塑的基本指导原则和要求、基本过程、基本方法做一比较全面和系统的分析和探讨,论述了艺术形象再塑这一文学翻译中普遍性问题的重要性,论证了只有从思想交流的实际出发、以读者的接受实践为依据、着眼于艺术再现的翻译,才能够造就既具社会价值、又具美学价值的译作珍品,从而达到为各民族文学与文化交流做贡献的最终目的。

The purpose of this work is to develop new methods for syntheses of polyimides derived from chlorophthalic anhydrides.

本工作的目的是以氯代苯酐和二胺为原料,制备双氯代酞酰亚胺单体,然后进行聚酰亚胺合成方法的研究,以达到减少合成步骤,降低其生产成本的目的,主要内容如下: 1由双氯代酞酰亚胺与双酚A在不同体系中进行聚合反应,发现双酚是否完全成盐和聚合体系含水量是决定聚合物分子量的关键因素。

Reefs, reef-mounds and lime mud mounds are all carbonate buildups, and the reefs and banks are important targets for petroleum exploration The reefs, banks and mounds should be divided from each other not only in theory, but also in exploration practice, because of so many differences in sedimentary compositions, fabrics, environments, distribution in time and space, and the characteristics of petroleum geology If the original reef or bank itself was fine reservoir rocks, then it would become a better reservoir after the reservoirconstructive diagenesis The formation of a high quality carbonate reservoir is controlled by two factors: the sedimentary microfacies and the reservoirconstructive diagenesis The first is the basis for the formation of a high quality reservoir, and with the high energy reefbank facies as the best; the latter includes the cataclasis, the dolomitization and the karstification The dolomitization and karstification are the best for the formation of a high quality reservoir The karstification has the widest distribution, profoundest effect, and plays a vital role in the high quality reservoir forming The karst types are quite rich in the limestone reef or bank reservoir, including synsedimentary karst, penesedimentary karst, supergene karst and hydrothermal karst, and so does the dolomitization reef or bank reservoir The formation mechanism of synsedimentary karst and penesedimentary karst is related to the short time exposing and leaching of the cyclical highfrequency shallowupward sequence when the reefs or banks are forming The supergene karst is controlled by the exposing and leaching of tertiary cyclic sequence type I interface after the reefs or banks forming Thus, there developed a great deal of secondary corrosion pores, corrosion fractures and huge caves besides some original pores remained in the reef and bank reservoir Scarcely there are any original effective pores in reefmounds and lime mud mounds, especially in senkes among mounds, but secondary pores could be formed by karstification, and there would likely develop source rocks with high organic abundance

礁、礁丘、丘同属碳酸盐建隆,礁、滩是重要勘探目的层。但各自在沉积组分、组构、环境和时空分布,以及油气地质条件上迥然不同,因而无论从理论还是勘探实践上,都有必要将其各成体系划分开来。礁滩自身为好储集岩,当建设性成岩作用叠加后可形成好的储层。碳酸盐岩优质储层形成,主要受沉积微相与建设性成岩作用两大因素控制。前者是基础,并以高能的礁滩相带为最好;后者主要包括破裂、白云石化和溶蚀三大类,并以白云石化或溶蚀为最优,其中的溶蚀分布最广泛、影响最深刻而对优质储层形成起决定性作用。礁滩储层中的古岩溶类型最为丰富多彩,包括同生期、准同生期、表生期和埋藏期热流体岩溶的所有类型,白云岩化礁滩体也不例外。其中,同生准同生期岩溶的形成机制与礁滩沉积时高频层序旋回向上变浅序列的短暂暴露淋溶有关,表生期岩溶则受控于礁滩沉积后三级层序旋回Ⅰ型界面的暴露淋溶。由此,导致礁滩储层中除了可以也是唯一保存原生孔隙外,还发育了次生溶蚀孔洞缝和大型溶洞。礁丘、灰泥丘尤其是丘间洼地中发育高有机质丰度烃源岩;它们自身没有有效的原生孔隙,但岩溶作用可以形成次生孔隙。

By means of either imagination or documentation , he has had as his writing purpose the keen-edged criticism.

在长达半个多世纪的&写作之旅&中,奈保尔以后殖民社会为主要写作对象,以想象与纪实为写作手段,以尖锐的批评为写作目的。

This text put forward 1 kind to blend technical fire to probe into calculate way according to the information, with feeling, feeling smoke, air with exposed to light and spread a feeling machine and probe in to imitate quantity for input, make use of rough gather a theoretical coordination degree calculate way to carry on fusion towards spreading feeling machine more signal, the purpose lies in designing a kind of fast and accurate with the valid fire probe into system to report to the police and lower a mistake to report to the police in advance by attaining of purpose

本文提出了一种基于信息融合技术的火灾探测算法,以感温、感烟、气体和感光传感器的探测模拟量为输入,利用粗糙集理论的协调度算法对多传感器信号进行融合,目的在于设计出一种快速、准确和有效的火灾探测系统,以达到提早报警和降低误报警的目的

Thirty advanced cancer patients who resident in the hospital in certain areas of Taipei and their families (N=60) were recruited. Using the revised TRIG questionnaire collected data. Family caregivers were interviewed for the qualification of the case respectively when the patients were still in the hospital and one month after the patients died. The questionnaire was analyzed via software SPSS.

本研究之目的在探讨癌症末期病人死亡地点不同与家属哀伤反应的相关性及探讨癌症末期病人死亡地点如愿与否与家属哀伤反应的相关性,采前瞻性研究法,以台北巿某区域医院之30对住院癌症末期病人及家属(N=60)为收案对象,以修改后的TRIG量表为工具,针对符合收案条件者分别於住院时及丧亲一个月后进行访谈,并完成问巻,结果以SPSS套装软体进行资料之统计分析。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。