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The pured mammary epithelial cell line could be obtained by 2~3 passages. 2.Using RPMI1640 containing 10黃, 100IU/ml Penicillin and 100IU/ml Streptomycin as basic media, several different growth factors such as insulin, hydrocortisone, insulin-like growth factor, EGF and so on, were added and selected to culture goat mammary epithelial cells.

以RPMI1640 为基础培养液,山羊乳腺上皮细胞在含血清、双抗的同时,添加氢化考的松、Insulin、IGF-1、E2、EGF 及ITS 的不同组合,以细胞生长群体倍增值为参考标准,评估不同组合的生物活性因子对山羊乳腺上皮细胞生长的影响。

The pured mammary epithelial cell line could be obtained by 2~3 passages. 2.Using RPMI1640 containing 10%FCS, 100IU/ml Penicillin and 100IU/ml Streptomycin as basic media, several different growth factors such as insulin, hydrocortisone, insulin-like growth factor, EGF and so on, were added and selected to culture goat mammary epithelial cells.

以RPMI1640 为基础培养液,山羊乳腺上皮细胞在含血清、双抗的同时,添加氢化考的松、Insulin、IGF-1、E2、EGF 及ITS 的不同组合,以细胞生长群体倍增值为参考标准,评估不同组合的生物活性因子对山羊乳腺上皮细胞生长的影响。

This paper, guided by the historical materialism of Marxism, seeks to explain Legge\'s Sinological Orientalism and his related translations by focusing on the related original works and extensive reading of these works.

本文坚持以马克思历史唯物主义的观点为指导思想,回归原典,以大量的原典阅读为基础,运用阐释学理论评述理雅各的汉学研究及其翻译作品。

Through simulation of the real process of thomcolumbar burst fracture and use of modem dynamics techniques, the present study was (1) to investigate the kinetic relationship between structural damage and impact energy absorption and dynamics mechanism of thoracolumbar burst fracture by quantitative analysis of various injured segments;(2) to explore the instability mechanism of L1 vertebral burst fracture and its injury threshold by three-dimensional analysis of the injured segment with stereophotogrammetry in combination of transient physical parameters, anatomy and image;(3) to explore the corresponding relationships between impact energy, geometry parameters and biomechanics by geometry and biomechanical analysis of thoracolumbar burst fracture, which may provide an objective standard for evaluation of spinal injury severity and experimental evidence for adoption of biomechanical treatment in clinic;(4) to evaluate the fixation effect of different instruments and the effect of intervertebral bone graft on segment fixation by analysis of the threedimensional stability of different internal fixation instruments and their decompression of the spinal canal, which may provide experimental evidence for therapeutical selection for thoracolumbar burst fracture in future; and finally to investigate the effect of various instrument fixation on dynamical characteristics by comparison and quantitative analysis of frequency and amplitude responses, which may provide certain theories and experimental evidence for application of vibration test to judge the stability of the spine.

本研究从胸腰段脊柱爆裂骨折发生的实际过程出发,以现代动力学检测及计算机多通道高性能数据采集分析系统等高新技术为基础,实时量化分析损伤节段的工程参数,揭示了结构破坏与能量吸收的变化规律,探讨了胸腰段脊柱爆裂骨折的瞬态损伤机制;利用三维立体摄像技术,对不同损伤程度的节段进行三维运动分析,结合瞬态物理参数、影像学和病理解剖,明确了〓椎体爆裂骨折的失稳机制及损伤阈值;通过对胸腰椎爆裂骨折的几何学变化及生物力学分析,明确了撞击能量与几何参数、几何参数与生物力学的相应关系,为评判脊柱损伤程度提供了客观标准,为治疗中采取适宜的生物力学方法提供了实验依据;通过对比分析不同内固定器械的三维稳定性和对椎管的减压作用,评价不同器械的复位固定作用,同时比较椎体间植骨对节段固定作用的影响,为今后临床胸腰段脊柱爆裂骨折的治疗选择提供依据;利用振动测试与分析技术,比较及量化分析了不同致伤状况的频幅响应特征,比较分析了各种器械固定对动力学特性的影响,为今后临床建立基于振动测试与分析技术判断脊柱稳定性的诊断方法,提供了一定的理论和实验依据。

This method has been applied to fluvial reservoirs of lower Minghuazhen Formation of Gangdong oil field, including setting high resolution isochronal stratigraphic framework on the base of intermediate term stratal cycle and predicting the location and type of sand bodies based on their size derived.

应用该方法对比黄骅坳陷港东油田明化镇组下段部分河流相储集层,首先建立以中期地层旋回为基础的等时地层格架,再以微相类型相同为对比约束条件,然后根据确定的砂体大小预测出砂体的位置和类型。

The Turbulence Slope calculations are based on the averaged tachogram and expressed in ms per RR interval.

计算振荡斜率 TS 时,以平均的阶梯图为基础, TS 值以每个 RR 间期多少毫秒为计量单位。

More importantly, the paper offers the theoric reference for people recognizing and evaluating properly the relationship between the Zang nationality and the Han nationality in the new age, and the Unitedmarriage between the Tang dynasty and the Tubo dynasty contributes greatly the historical development to China and the world.

本文以马克思主义的唯物史观和民族理论为指导,以丰富的藏汉文史料为基础,引入一些史学界目前仍有争议的观点,搜集了大量的民间文学,力求使论文内容完备,观点新颖,可读性强。

1Theoretically,based on Marxism and positive methodology and applying heavily theory andmethodologies from modern political science、sociology and Social psychology,it provides a more pro-found analysis on Sun's political thought;2as far as the narrative is concerned,the essay does not setany abstract goal to give a unpositive judgement on Sun's thought but sets the study in the social envi-ronment then and in an enviroment where international political thought kept changing;3the essayalso provides new ideas elsewhere as far as individual issues are concerned.

本文的独特之处在于:一、从理论而言,本文以马克思主义为基本指导,以实证史学为基础,大量运用了现代政治学、社会学、社会心理学等理论与方法,对孙中山的政治思想作了较为深入的分析。二、从论述角度而言,本文并未设定一抽象目标,对孙中山妄加评判,而是把孙中山放在中国当时的社会环境,国际政治思潮变化的环境中进行分析。三、从具体问题而言,本文也有若干新见。

Results The former unstandardized,loose customs have been effectively changed by running the system under the quality management.

以ISO9000标准的管理思想为指导,以优质、安全的血液和血液制品为基础,建立血站的质量管理体系。

In this dissertation, the author analyzes corporate opportunity doctrine deeply on the base of the basic theory, and it focus on defining corporate opportunity doctrine and regulating usurping corporate opportunity doctrine. In this dissertation, the author uses the comprehensive analysis methods such as empirical analysis method, comparative analysis method, value analysis method and standardized analysis method.

本文综合运用了法律的实证分析、比较分析、价值分析及规范分析的研究方法,以公司机会规则的一般理论为基础,以公司机会的认定和篡夺公司机会的法律规制为重心,较为深入地分析了公司机会规则。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。