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代谢物

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These closely related Gibberella species differ in their reproductive behaviors, geographic distribution and host ranges, plant pathogenesis, and production of toxins and other biologically active metabolites.

虽然它们是非常近缘的赤霉属各种,但在繁殖方式、地理分布、寄主范围、植物致病机理、毒素和其他生物活性代谢物的产生方面都有所不同。

Objective: In order to provide sensitive and reliable technique for the study of the relationship between enviromental estrogens and diseases, analytical methods for three kinds of representative environmental estrogens such as mycotoxins (zearalenone and its metabolites, aflatoxin B_1 and ochratoxin A), persistent pesticide pollutants (hexachlorocyclohexane and dichlorodiphenyltrichloroethane) and industrial chemicals (bisphenol A and 4-nonylphenol) with chromatography were to develop.

目的:选择三种有代表性的环境雌激素:真菌类毒素(玉米赤霉烯酮及其代谢物及赭曲霉毒素A、黄曲霉毒素B_1)、农药类持久性污染物(666、DDT)和化工原料类雌激素为对象,分别研究它们的检测方法,为监控环境激素和研究它们与疾病之间关系提供灵敏、可靠的分析方法和实验依据。

Type II diabetes patients had higher urinary lactate, glycine, TMAO and hippurate values than that of combined non-vegetarian and vegetarian group.

荤食、素食混合组与第二型糖尿病者其尿液中代谢物比较,显示出第二型糖尿病人其尿液中lactate、glycine、TMAO及hippurate值均较荤、素食混合组者高。

For those out-patients of Kaohsiung Medical University Hospital and normal non-vegetarian, vegetarian and college students, we investigate the effect of type II diabetes, non-vegetarian, vegetarian, age and gender on the NMR values of urinary metabolites including lactate, alanine, acetate, glycine, acetone, citrate, TMAO, DMA and hippurate.

本研究针对高雄医学大学附设医院门诊第二型糖尿病患及正常荤食、素食者、大学生利用1H-NMR核磁共振,探讨荤食、素食、年纪、性别及第二型糖尿病患者,其对尿中代谢物包括alanine、lactate、glycine、citrate、acetone、acetate、dimetylamine (DMA、trimethlamine –N-oxide、hippuric acid值之影响。

Six experimental stages were designed in our procedure, those are:(1) metabolite recovery and tested sample preparation: the metabolites were recovered by Amberlite XAD-2 absorption, followed by MeOH elution and solvent concentration;(2) antioxidant detection and strain selection: samples were quantitatively analyzed by the inhibition effects on formation of lipid peroxides and TBARS to screen the strains able to produce antioxidants. According to the established screening methods, we chose out a strain of actinomycetes, designed as AMBL-029C;(3) antioxidant purification: the fermentation broth was recovered by a series of separation techniques including centrifugation, Amberlite XAD-2 absorption, followed by MeOH elution and a successive TLC purification. The resulting primary purified compound [temperately designed as AMBL-029C-TS] was further analyzed by HPLC to monitor its purity;(4) physical-chemical characteristics: judging from the acid-base fractionation experiments, and the pH and temperature stability tests, the compound was deduced to be a acidic compound with the properties of low polarity and highly pH and temperature stable;(5) mechanism of the antioxidant: in comparison with some other known antioxidants, TS was subjected to investigate its antioxidant mechanism, together with BHT,-tocopherol, as well as two streptomyces metabolites, homogentisic acid and -phenylpyruvic acid, which were previously isolated as the natural antioxidants in our laboratory.

针对本实验目的,我们设计了以下的实验步骤﹔(1)二次代谢物回收及检测样本处理:我们将发酵所得的培养上清液,利用疏水吸附性树酯Amberlite XAD-2吸附回收,并以甲醇溶离及真空减压浓缩脱水等方式处理,以取得提供抗氧化活性筛选之检测样本;(2)抗氧化活性检测及菌种筛选:以「过氧化脂质」和「硫丙二醯尿」的生成量进行定性定量分析以作为抗氧化物质生产菌筛选之用;经此筛选程序,我们选获了具有抗氧化物质高生产力的菌株,命名为AMBL-029C;(3)抗氧化物质的分离纯化:针对生产菌株的发酵回收处理液,以矽胶薄层色层分离法经物质层析纯化后,并以高效能液相层析法(High performance liquid chromatography;HPLC)分析物质可得一初级纯化物质,命名为AMBL-029C-TS;(4)抗氧化物质的物理化学性质分析:由酸碱转溶(acid-base fractionation)实验得知,此抗氧化物质属於中低极性的强酸性物质,对温度(37℃-100℃)及酸碱度(pH3.0-13.0)均表现出高稳定性;(5)在抗氧化机制探讨方面,我们针对数种不同的抗氧化机制进行探讨,即: 1。

With high--performence liquid chromatography, the contents of amino acids in the hippocampus and dopamine、homovanillic acid in the subcortical tissue of the rats chronically treated with methylphenidate or ketamine were assesed.

并利用高效液相色谱分析法测定经哌甲酯、氯安酮长期处理后的大鼠海马游离氨基酸和皮层下组织多巴胺及代谢物的含量。

In this study, the amount of ethylene and its key biosynthesis genes expression, typical allelochemical hydroxamic acid concentration and the key regulation genes (BX1 and BX9), as well as the dynamic expression and time of relevant defense key genes of secondary substance metabolism processes were compared in ethephon treatments of different concentration and duration.

为探明外源乙烯利对玉米化学防御作用诱导的浓度和时间效应,本文采用化学和分子生物学相结合的方法,研究了不同浓度乙烯利处理玉米后,叶片乙烯释放量及其合成调控关键基因ACS和ACO表达的变化,典型化感物质丁布的含量及其关键调控基因BX1和BX9表达的变化,以及其他调控次生代谢物的关键基因表达的时间动态。

KETO is a natural metabolite of DHEA that has all of the benefits but none of the side effects associated with DHEA.

它是一种天然的DHEA的代谢物,而且没有任何副作用。

The metabolites of NAA, Cr, Cho and Lac were analyzed.

主要观察的代谢物为NAA,Cr,Cho和Lac。

This is the first report regarding the study of genetic transformation of Pueraria lobata, P.

本文首次报道发根农杆菌对野葛、山葛和三裂叶葛的遗传转化,野葛毛状根培养及其次生代谢物的研究,主要结果如下

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