代谢

The chemical shifts and concentrations of the metabolites in the serum were measured in serum samples with pH varied between 7.0 and 7.8. It was demonstrated that, from pH 7.0 to pH 7.8, the chemical shifts of many low molecular-weight metabolites drifted upfield and the intensities of phenylalanine, tyrosine, histidine, and lactate in the T 2-weighted NMR spectra changed significantly.

实验结果表明 pH的改变不仅能够引起血清中一些小分子代谢物化学位移的改变，还会影响小分子代谢物与蛋白的相互作用，引起这些小分子结合态和游离态含量的变化。

It contents with uracil in the same enzyme system,blocks the metabolic link,impeds the synthesis of DNA,and then prohibits the propagation of the cancer cells.

它的结构与肿瘤细胞所必需的代谢结构尿嘧啶相类似，在同一系统酶中互相竞争，阻断代谢环节，阻碍DNA的合成，从而抑制肿瘤细胞的增殖。

The SOD and the LDH-1 activities and EST (R_f=0.17 and R_f=0.72) at 1℃weresignificantly higher than the values of the room temperature(P＜0.05), but the total enzymeactivities of LDH, G6PDH and EST at room temperature and 1℃were similar(P＞0.05).This showed well-developed anoxia tolerance of the Heilongjiang wood frog to maintainplasma viability when low temperature interrupts the delivery of oxygen and substrates. LDH-1rose significantly avoided the liver lactic acid accumulation that may created the liver to bewearied. EST (R_f=0.17 and R_f=0.72) rose may enhance the ester metabolism to offer muchenergy. These showed steady metabolic rate and relative stable organism environment of theHeilongjiang wood frog to survive under low temperature.Native PAGE separated proteins of liver tissue successfully on the better condition is 6％final gel concentration and there were 15～19 bands identified on this condition.

同功酶电泳图谱显示，1℃组与室温组黑龙江林蛙肝脏LDH酶活性差异不显著，均以LDH-5为主，但1℃组LDH-1显著高于室温组(P＜0.05)，推测黑龙江林蛙为适应低温低氧环境，增强LDH-1酶活性以较好的利用乳酸，避免因大量乳酸形成造成肝脏疲劳；而1℃组肝脏G6PDH酶活性无显著变化，进一步说明低温条件下葡萄糖戊糖途径和糖酵解作用的互相协调，维持机体代谢的稳定；1℃组和室温组EST总酶比活力无显著变化，但1℃组中R_f=0.17和R_f=0.72的EST同工酶显著升高(P＜0.05)，推测在低温条件下黑龙江林蛙可能通过选择性增强部分酯酶同功酶活性，增加肝脏酯类物质代谢，为机体提供维持生命必需的最低能量。

The last part of this article emphasis on the mechanism of cichory extract N2. Hepatic lipase, NO and xanthine oxidase are observed in this aspect, and gene expression of hepatic lipase is also detected.

本部分研究观察了菊苣提取物 N2 对影响脂质代谢的肝脂酶、尿酸代谢的关键酶黄嘌呤氧化酶、一氧化氮的活性和含量，还从分子生物学角度观察了肝脂酶的基因表达。

Gas chromatography was employed to quantify the output of metabolization,and it was found that the yield of diacetyl for the mutant UV-3 was 18.7 fold to the wild-type 10293,up to 1.045g·L~(-1).Acetoin and ethanol yields were decreased by 48.4%and 71.4%,respectively.The yield of acetate was increased by 34.6%.

利用气相色谱测定代谢产物量，分析诱变前后菌株代谢途径中碳源的流向，结果表明突变株UV-3的丁二酮产量提高18.7倍，达到1.045g/L，乙偶姻产量降低48.4%，乙醇产量降低71.4%，乙酸产量提高34.6%，且遗传性质稳定。

Therefore,K. pneumoniae CICC 10011 is a potential strain for 2,3-BD production.Thirdly,a novel aqueous two-phase system for the recovery of 2,3-BD from fermentation broth was developed employing isopropanol/(NH_4)_2SO_4 system.The results showed that most of target products(2,3-BD and acetoin) can be partitioned efficiently into the top phase,while most of glucose can be partitioned into the bottom phase.

通过对批式流加发酵过程中两株克雷伯氏菌代谢流量的分析表明，K.pneumoniaeCICC 10011代谢产生的各种有机酸和乙醇浓度均明显低于K.pneumoniae DSM 2026，而目标产物浓度以及产物转化率却相对较高，是发酵2，3-丁二醇的优良菌株。

Methods Fifteen pigs were anaesthetized, and trachea intubation and transfemoral cannula were performed then. Swan-Ganz catheters were inserted to pulmonary artery via internal jugular vein, and aeroperitoneum was set up by airing CO2. These pigs were randomly divided into three groups (5 pigs in each group) according to different levels of IAP （IAP10, IAP20, IAP30）. Hemodynamics and oxygen metabolism values were observed and recorded before airing, 6, 12, 18 and 24 hours after airing, respectively.

15只猪经麻醉后，行气管插管、股动脉插管及经颈内静脉肺动脉插管，CO2充气建立气腹，根据随机数字表法将猪分为IAP 10 cm H2O组(IAP10组)、IAP20组及IAP30组（每组5只），记录充气前，充气后6、12、18及24 h的血流动力学和氧代谢指标，观察不同水平IAP在不同时点上对血流动力学及氧代谢的影响。

The fitting culture conditions for isolate BC4 producing more toxin were Improvement Fries and Czapek-Doxbiotion culture medium , culture time for thirty-five days, pH for 6, culture temperature for 25 , still cultivation, and that the juice of tomato and pepper cannot improve herbicidal activity on Amaranthus retroflexus.

试验确定了菌株BC4代谢产物中除草活性物质产生的适宜条件：即改良Fries培养基和查氏-道氏培养基：培养时间是35d；pH值为6；培养温度为25℃；静止培养；培养基中添加一定比例的番茄、辣椒的浸出汁并未提高灰葡萄孢代谢产物的除草活性。

GC-MS or LC-MS is a kind of modern,fair chemical analytical technique,which combine higher separation ofChromatography with strong identification of Mass.It was applied in new drug research including concentration determination,impuritycheck,pharmacokinetic,anabolite analysis and so on.The paper reviews their application examples for recent years and shows itsdevelopment future.

气一质联用法和液一质联用法将色谱的高分离性能和质谱的高鉴别特点相结合，组成了较完美的现代分析技术，在新药研究的含量测定，有关物质检查、质量标准制定以及药物动力学研究的代谢物分析、药物及代谢物的体内浓度分布等试验中，成为有力的分析工具，文章综述了近年来色谱一质谱联用在以上领域的应用实例，展示了它的发展前景。

This is because anabolic/androgenic steroids may interfere with the ability for the body to clear corticosteroids from circulation, due to the fact that in their respective pathways of metabolism these hormones share certain enzymes.

这是因为合成代谢类/雄激素类固醇可能会干扰能力的机构，以明确的皮质类固醇的流通，这是由于在各自的途径分享这些激素代谢的某些酶。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失