代谢

According to experimental results and etiopathogenisis and pathology of osteoporosis , and analyzing review investigation on hepcidin、associations of bone metabolism and iron metabolism and relations of ferri ion and calcium ion,we can make conclusions as follows: 1、We applied single manmade intervention approach to induce osteoporosis model in this study, there was no other influential factor. In the termination of test , rats bone density and bone histological anatomy indicated that osteoporosis model was creditable,meanwhile hepatic functional enzyme and hepatic microtome section demonstrated that hepatic function had noci-influence induced by medicinal herbs resource. Accordingly, we could believe that hepatic hepcidin gene expression and serum hepcidin contents can represent internal iron metabolism changes at selected test time. 2、In this investigation, different findings between test group and control group indicated that:according to changes of hepcidin gene expression measured by RT-PCR and changes of serum hepcidin contents determined by ELISA kit , there were correlations between hepcidin and rat osteoporosis model induced by retinoic acid. 3、Under the condition of this investigation,there was a interactive hypothesis as follows: Hepcidin iron metabolism←→ferriion←→calcium ion←→osteoporosis.

根据本次实验结果，对骨质疏松的病因病理的认识及铁代谢与铁调素、骨代谢与铁代谢关系、铁离子与钙离子的关系系列研究的回顾，经分析得出如下结论：1、本研究骨质疏松模型采用单一人工干预方法，没有其他影响因素；实验结束时大鼠骨密度、骨组织学检查表明骨质疏松形成，同时肝脏功能酶和HE切片显示肝脏功能未受药源性产生明显损伤性影响；因此，同期不同时间组大鼠肝脏铁调素基因表达、血清铁调素含量可以代表体内铁代谢不同时间的变化。2、在本研究中，模型组与对照组研究结果的差异表明：铁调素基因RT-PCR变化、血清铁调素ELISA测定含量变化与维甲酸制作的大鼠骨质疏松模型形成有实验相关性。3、在本研究平台下，形成了一个互相影响的假设图：铁调素←→铁代谢←→铁离子←→钙离子骨质疏松。

A reciprocal metabolism pattern was represented between cortices and paralimic regions, which implicated a reciprocal dysfunctional changes of paralimic-cortex pathway in patients with TRD. Cerebellar hypermetabolism suggested hyperfunctional changes of cerebellum under depressed condition. Frontal white matter hypermetabolism implicated further studies on potential structural abnormalities of cerebral regions in TRD.

难治性抑郁症患者存在旁边缘系统代谢增高和皮质代谢降低的交互性代谢改变模式，支持抑郁症边缘系统－皮质功能紊乱假说；患者小脑代谢水平增高，提示抑郁状态下小脑功能亢进；额叶白质高代谢水平提示有必要对难治性抑郁症潜在的脑结构异常改变进行深入研究。

The comparisons of biochemistry between OP and OR rats The differences between OP and OR rats included not only body weight, but lipids metabolism and insulin sensitivity as well, characterized with insulin resistance, increasing in serum free fatty acids and ketone body, and hepatic TC and TG in OP rats. However, no significant differences were observed in serum TG, TC, LDL, HDL and fasting glucose between OP and OR rats.⑶Comparisons of metabolites in serum, urine and liver tissue between OP and OR rats①There were significant differences in amino acids concentration between OP and OR rats,especially in liver tissue, such as high concentrations in ketogenic and glucogenic amino acids in OP rats, suggesting differences in amino acids metabolism;② The different metabolites between OP and OR rats included increasing of various saturated fatty acids and decreasing of polyunsaturated fatty acids in OP rats;③The urinary metabolites analysis indicated that different structure or metabolism of gut microflora might exist between the two phenotypes, which probably influenced the regulation of body weight gain;④The end-products of catecholamines in urine and intermediates of krebs cycle in serum in OP rats were all up-regulated, suggesting that the activity of sympatheic nervous system and energy metabolism was higher in OP rats than OR rats.

胰岛素耐受实验和胰岛素敏感指数表明OP动物的胰岛素敏感性较OR动物下降，而OP大鼠血清中游离脂肪酸、酮体、肝脏总胆固醇和甘油三酯水平显著升高；但是，OP与OR大鼠血清中总胆固醇、甘油三酯、低密度脂蛋白、高密度脂蛋白和空腹血糖等的水平并无显著性差异；⑶肥胖易感与肥胖抵抗大鼠血清、尿液和肝脏组织提取物中代谢物的比较研究表明：①OP与OR大鼠的血清、尿液和肝组织提取物中多种氨基酸的含量存在显著差异，并以肝组织中的差异氨基酸数量为最多，包括各种生酮和生糖氨基酸水平在OP组的升高，说明氨基酸代谢的差异是两种体重表型大鼠之间存在的重要差异特征之一；②OP与OR动物肝脏和血清差异代谢物中包含多种饱和长链脂肪酸的升高如十四烷酸、十六烷酸、硬脂酸等和多不饱和脂肪酸的下降如亚油酸和花生四烯酸，说明两种体重表型动物的肝脏脂肪酸代谢存在明显差异；③长期高脂饮食喂养后，动物的尿液代谢物分析表明OP与OR动物体内的肠道菌群结构存在差异，这些菌群上的差别可能在动物体重增长的调节上产生影响；④与OR动物相比，OP动物尿液代谢物中儿茶酚胺类递质的代谢终产物如高香草酸、扁桃酸和4-羟基苯乙酸明显升高。

The comparative transcript profiles between OP and OR rats reveal some differentially expressed genes involved in lipids metabolism, ketone body production, blood pressure regulation and adipoctyes differentiation processes.The combined strategy of metabonomics and transcriptomics provides comprehensive information of metabolic characters of obesity and corresponding mechanisms to obese-related metabolic disorders from a systematic view, e.g.

高脂饮食喂养建立的OP大鼠体内的整体代谢特征与OR大鼠不同，具体包括：交感神经系统活性、能量代谢、脂肪酸代谢、氨基酸代谢以及肠道菌群结构等；OP和OR两种表型的转录组学比较也揭示了部分参与脂质代谢、酮体生成、血压调节和脂肪细胞分化等调控过程的基因存在差异性表达。

As tail-suspended rats model can form an abnormal model of lipid metabolism as well as bone metabolism, We added three levels offish oil to the diets of rats of this model to determine the effects of n-3 polyunsaturated fatty acids on lipid and bone metabolism, so as to determine the interaction of these two chronic diseases and clarify the mechanism of these diseases.

尾部悬吊模拟失重大鼠模型能够同时造成大鼠脂质代谢与骨代谢失调，而应用此模型进行脂质代谢与骨代谢失调相关关系及膳食(n-3)多不饱和脂肪酸干预对模拟失重条件下脂质与骨代谢的影响的研究尚未见报道。本实验通过添加以鱼油为来源的不同剂量的(n-3)多不饱和脂肪酸作为膳食干预手段，研究其对模拟失重条件下构建的大鼠脂质代谢与骨代谢异常的调节作用，以加强对脂质代谢与骨代谢相互作用生理进程的深入了解为目标，为阐明中老年人群慢性疾病并发或多发的生理生化机制提供一定的实验依据。

objective to explore environment risk factors of metabolic syndrome.methods a cross-sectional population survey with questionnaires investigation,checkup and laboratory measurement for metabolic syndrome was performed among 2026 teachers,and the logistic regression was used to analyze the risk factors of ms.results the education,milk intakes,fish and aquatic products intakes,body exercise and drinking tea were different significantly between male and female individuals.univariate unconditional logistic regression analysis showed that education,body exercises,fish and aquatic products intakes and drinking tea were benefited to the ms,but the age,sucrose intakes were the risk factors to the ms.the multivariate logistic stepwise regression analysis showed that compared with individuals with no drinking milk,or no fish and aquatic product intake,or no drinking tea,or no sucrose intakes,the milk intakes 250-1500 g/week(or=0.731,95%ci:0.542-0.987),the fish and aquatic product intake with 250-1000 g/week(or=0.720,95%ci: 0.541-0.959),or sucrose intakes 250 g/month(or=0.446,95%ci:0.255-0.779),drinking tea forepassed(or=0.635,95%ci:0.458-0.883),and current (or=2.084,95%ci:1.390-3.125) had different levels of risk on ms.conclusion the age and sucrose intakes are risk factors,and the milk intakes,aquatic products and drinking tea benefits for ms.

目的 了解中小学教师代谢综合征发病及其影响因素。方法采用横断面调查方法，随机抽取芜湖市中小学教师2 026名，进行问卷调查、体格检查和实验室检测，并运用logistic回归分析代谢综合征影响因素。结果不同性别的中小学教师在受教育程度、牛奶摄入、水产品摄入和饮茶习惯等方面的差异有统计学意义；其中受教育程度、体育锻炼、水产品摄入和饮茶是保护因素，而年龄、工作紧张程度、糖的摄入可能是代谢综合征的危险因素。logistic逐步回归分析结果提示，牛奶摄入、水产品摄入和饮茶是代谢综合征的保护因素，而年龄和糖摄入是代谢综合征的危险因素，其中牛奶摄入在250～1500 g/周(or=0.731，95% ci=0.542～0.987)，水产品摄入在250～1000 g/周(or=0.720，95% ci=0.541～0.959)以及以前饮茶（or=0.446，95% ci=0.255～0.779和现在正在饮茶(or=0.635，95% ci=0.458～0.883)，对代谢综合征的保护作用明显，而糖摄入250 g/月时代谢综合征的患病的危险是不食用食糖的2倍(or=2.084，95% ci=1.390～3.125)。结论年龄和糖摄入是代谢综合征患病的危险因素，而牛奶、水产品和饮茶是代谢综合征的保护因素。

To our knowledge, reverse phase of high performance liquid chromatography was firstly developed to analyze the metabolites of 7-tocopherol with ideal results. Meanwhile, Gas chromatography/Mass chromatography were used to analyze the structures of unknown metabolites of 7-tocopherol and a main unknown metabolite was identified as a side chain ω- hydroxylate of 7-tocopherol.The kinetic results of of 7-tocopherol reacted in the S9 of male SD-rat liver showed that the optimal time was 45 min; the optimal concentration of S9 5 mg/ml; the optimal concentration of NADPH 1 mM; and the optimal concentration of 7-tocopherol 8 μg/ml. The result of γ-tocopherol metabolized in the S9 of male SD-rat was the best; male Brazil turtle better; and male cobia worst.

本试验首次确立了γ-生育酚代谢物的RP-HPLC分析方法，结果理想且重现性好；用GC／MS对γ-生育酚的未知代谢物结构进行分析，判断其中一种主要的代谢物为γ-生育酚侧链初级羟基化代谢物；γ-生育酚在雄性SD-大鼠肝S9的代谢动力学表明，本实验条件下代谢反应的最佳时间为45min，S9浓度为5mg／ml，NADPH浓度为1mM，γ-生育酚浓度为8μg／ml；发现γ-生育酚在雄性SD-大鼠肝S9中代谢最高，雄性巴西龟次之，雄性军曹鱼最低。

Investigation of major metabolic pathways of SFZ-47 in animals The metabolites of SFZ-47 in rabbit urine following oral administration were separated and detected with HPLC and LC-MS methods. On the basis of the chromatographic behavior and mass spectra of the metabolites as well as biotransformation pathways of drugs with the partial structure of SFZ-47, the hydroxylic derivative 4-(3H-1, 2- dihydro-1-pyrrolizinone-2-methylamino benzyl alcohol and the carboxylic derivative 4-(3H-1, 2-dihydro-1-pyrrolizinone-2-methylamino benzoic acid of SFZ-47 were proposed as two potential metabolites and were synthesized.

在动物体内主要代谢途径的确定用HPLC和LC-MS对家兔口服SFZ-47后尿中的代谢物进行了分离、检测，根据代谢产物的色谱和质谱行为以及与SFZ-47有类似结构部分的其它药物的代谢途径，推测其羟基衍生物[4-（3H-1，2-二氢-1-吡咯里嗪酮-2-甲基胺基）苯甲醇]与羧基衍生物[4-（3H-1，2-二氢-1-吡咯里嗪酮-2-甲基胺基）苯甲酸]是两种可能的代谢产物，遂用化学方法合成了这两种衍生物。

Results Oxygen metabolism abnormality was found after trauma, and it was correlated with ISS, RTS, injured organ or region and number of injured organs, shock, systemic inflammatory response syndrome and respiratory complications. It was more intense in the patients with MODS. There was marked difference in the ratio of change in oxygen metabolism between MODS and NMODS groups. Oxygen deficiency metabolic variables tended to deteriorate in the nonsurvival group. More marked changes in metabolic variables indicated severer organ dysfunction, reaching their peak values before death.

结果 氧代谢水平在创伤后即发生异常，与创伤严重度评分、RTS、损伤器官部位与数量、低血容量性休克、全身炎症反应综合征以及是否并发呼吸系统合并症等有关；并发MODS患者氧代谢水平变化更为明显，与轻伤对照组和NMODS组比较相关氧代谢比值改变差异均有显著性；死亡组乏氧代谢指标常呈持续恶化趋势，其代谢水平改变越显著，预示器官功能损害程度越严重，往往于死亡前达到峰值。

Firstly, based on phenomenal characteristic parameter collection and its further correlation analysis, the metabolic flux shift during late phase of guanosine fermentation was preliminarily deduced and confirmed by combining intermediates detection and the product biosynthesis pathway analysis. That is, the metabolic flux shifted from Hexose Monophosphate Pathway , from which guanosine was generated mainly, to Embden-Meyerhof Pathway and Tricarboxylic acid cycle to form amino acids and organic acids.

首先通过对发酵过程现象特征的收集和进一步的相关分析，初步推断在发酵过程后期存在有代谢流的迁移，结合中间代谢产物的分析和产物合成的代谢途径初步确认了代谢流迁移的存在，即代谢流从形成产物的HMP途径向形成有机酸、氨基酸的EMP途径、TCA循环迁移。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失