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Taking advantage of the techniques of osteoblastic culture, we can observe the direct effect of TKR on osteoblast.

取第3代细胞,培养24h,换含不同分离组份的培养液,继续培养72h,细胞增殖检测用MTT法。

Alkyl manganese pentacarbonyl compounds5MnR (R = CH3, p-CH2C6H4CH3, p-CH2C6H4OCH3) were synthesized by metalation of manganese pentacarbony potassium salt with corresponding alkyl halides in ether. Photolysis of these with 1-2 equivalent of (CH3)2(C6H5)SiH or (CH3)(C6H5)2SiH in C6D6 solution at 5℃ produces silyl manganese pentacarbonyl compounds5MnSi(C6H5)(CH3)2 and5MnSi(C6H5)2(CH3) respectively (yield 70-88%).

用五羰基锰钾盐和相应的卤代物在乙醚中的金属化反应合成了五羰基锰烷基合物5MnR(R = CH3,p-CH2C6H4CH3, p-CH2C6H4OCH3 ),产率达到72-93%,将这些化合物与1-2当量(CH3)2(C6H5)SiH和(CH3)(C6H5)2SiH的C6D6溶液在5℃光解,分别得到五羰基锰硅烷基化合物5MnSi(C6H5)(CH3)2和5MnSi(C6H5)2(CH3)(产率达到70-88%)。

Another method for determining primary structure is to employ Edrnan's , which reacts quantitatively with the free amino group of a peptide to yield the corresponding pheny lthiocarbamoyl peptide, leaving the rest of the peptide chain intact.

另一种可以决定初级结构的方法是用埃德曼降解法,它们能与肽键上的游离氨基定量反应,产生相应的苯氨基硫代甲酰肽,而其余的肽链保持不变。

None of them have been reported before. Some efforts were also made to find cupric ion as the right catayzer which could promote the cyclization of phenylhydrazine and a-bromoacetophenone efficiently.

为了扩展这一新的合成方法,我们用苯肼替代甲肼,发现在合适的催化剂存在下,苯肼也能够与α-溴代苯乙酮发生类似的环合反应,得到2,4-二苯基-1,2,3-三氮唑。

The normally brominated Baylis-Hillman adducts were formed as the major product at low temperature. While at room temperature or heating, the α,γand β,β-dibrominated compounds were formed as the major products using 3-butyn-2-one and methyl propiolate or propynenitrile as substrate, respectively.

贫电子炔类化合物的Baylis-Hillman反应的研究在这部分工作中我们发现在用TiBr〓或BBr〓为Lewis酸催化贫电子炔类化合物和芳香醛的Baylis-Hillman反应时,温度对反应的影响很大:当反应在低温下进行时,通常生成的是正常的β-溴代的Baylis-Hillman反应产物;而在室温或加热条件下反应,以丁炔酮为底物时,主要生成的是α,γ-二溴化合物,而以丙炔酸甲酯及丙炔腈为底物时,主要生成的是β,β-二溴化合物。

In the third chapter, the synthesis of5-[(1"S)-1",2"-diacetyl-oxyethyl]-1-phenylpyrazol-3-carboxaldehyde oxime(2) from5-[(1"S)-1",2"-diacetyl-oxyethyl]-1-phenylpyrazol-3-carboxaldehyde(1) and phenyl-hydrazine were introduced firstly. The synthesis of nine dioxapyrrolino[3,4-c]pyrazoles containing chiral pyrazolyl moiety(6a-i) from 2 with N-substituted-phenylmaleimide by nonmetal oxidant IBD-initiated intermolecular 1,3-dipolarcycloaddition was described in detail.

第三章首先以5-[(1′S)-1′,2′-二乙酰氧基乙基]-1-苯基吡唑-3-醛(1)和苯肼为原料,合成了化合物5-(1′S)-1′,2′-二乙酰氧基乙基1-1-苯基吡唑-3-醛-苯腙(2);再用化合物(2)与N-取代苯基马来酰亚胺反应,利用无金属氧化剂—IBD引发1,3-偶极反应,发生分子间的环加成,合成了9种新型手性吡唑连二氧代吡咯啉并

RESULTS: After differentiation of human adherent myoblasts by neural induction medium, no cells with a neural cell morphology (ie., small, refractile cell body with dendritic cell extensions) were seen. All remaining myoblasts cultured with neural induction medium, myoblasts with proliferation medium and myotubes with differentiation medium containing 20 mL/L HS were positive for β Tubulin Ⅲ. C2C12 myoblasts were negative for β Tubulin Ⅲ. In contrast, all the above cells were negative for the markers Neurofilament Mr 68×103 and GFAP.

结果:用诱导分化液作用后,未见小的、伴有突起的放射状形态的神经细胞;抗β Tubulin Ⅲ对经神经元胶质细胞诱导分化液作用后的人成肌细胞、增殖培养液培养后的各代人成肌细胞及仅含20 mL/L HS分化液分化形成的肌管细胞染色均为阳性; C2C12细胞β Tubulin Ⅲ抗体染色阴性;上述所有细胞抗Neurofilament Mr 68×103和抗GFAP染色均为阴性。

It is expressed as a function of the temperature expansion coefficients of the crystal lattice, their remanent polarization and other parameters.

用&纯&PbZr_xTi_(1-xO_3的实验数据代入表达式计算,得到准同型相界附近组成的T_对Zr/Ti的关系曲线,与实验结果较好地符合。

Third, on the base of Construction Grammar, this thesis not only uses metalanguage to interpret the vague event, but also retrieves the semantic components from the whole construction. In other words, this thesis postulates: although these three verbs are not full fledged pro-verbs in some cases, they behave like pro-verbs that they are 'super-lexical' morphemes and act differently from other predicates which just render a situation type for the event. For example, the frame-evoking verbs may change the situation type of an activity into a resultative state, or imply that the speaker takes business as an informal event in a rather pejorative tone.

再者,本文除了发现格式语法可以透过后设语言明确描述隐含的事件,并且可以透过对整个格式语意成分的提取,尽管在某些情况下尚不成熟,但可将这三个常项动词设定成整个语意框架内的「代动词」,换句话说,这三个动词的地位是在一般词汇之上,因为他们不像一般动词那样只给所带出的事件一个「动相类型」,而是可以提供整个语意框架较多的语意诠释:可能是一个事件状态的改变,例如:产生一个「结果状态」,或是使整个框架得到一个作说者对待事件的「角度」,例如:作说者用比较「贬义的角度」来看待该隐含的事件等等。

The dogs were administrated with carboplatin at the dosage of 16.5mg/kg intravenously, intra-arterially or retroperitoneally. The blood samples were taken at 0, 5, 15 minutes, and 0.5, 1, 2, 4, 8, 24 and 72 hours after instillation. The competent platinum concentrations were measured with high-performance liquid chromatography. Then the pharmacokinetic parameters were calculated and analysed.

随机将18只雌性家犬分为3组,分别经静脉,动脉及腹膜后推注卡铂(16.5 mg/kg),然后于给药后的0、5、15分钟,0.5、1、2、4、8、24、72小时取静脉血,用高效液相色谱法测定血浆活性铂的浓度,计算和比较不同给药方式下卡铂的药代动力学参数。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。