代用

By combining resolution and protection of DBSPINOL with menthyl chloroformate, the enantiopure SPINOL and other 4,4'-derivatives have been synthesized effectively via (1S)--4,4'-dibromo-l,r-spirobiindane-7,7'-diyl bis-menthyl carbonate with n-BuLi following reacting with electrophiles of I2, MeI, CO2 and water.

在制备其它衍生物反应中，薄荷醇碳酸酯成为羟基的保护基，DBSPINOL碳酸酯用正丁基锂处理后，再和亲电试剂作用合成了其它4，4'位二碘代、二甲基取代和二羧基取代SPINOL新化合物。

Methods: 1. A model of Transwell has been developed for the co-culture nearly pure microglia cells and dopaminergic neurons.

研究方法 1、用原代培养的方法建立小胶质细胞与中脑多巴胺能神经元体外培养的Transwell细胞模型。

Hardy was an excellent novelist in the end of 19 century and Tess of the d'Urbervilles was the most representative novel of his . It narrated the short and misfortunate life of a rural woman .

德伯家的苔丝》是19世纪末英国杰出现实主义作家哈代最有代表性的小说，它用史诗一般的文字描写了贫穷的农家女子苔丝短促而不幸的一生。

The results showed that the three kinds of prealloy powder have excellent low temperature sintering characteristics and good wear resistance. They have excellent physical and mechanical characteristics close to ultrafine cobalt and imported prealloy powder, good retention to diamond and excellent bonding momental for high frequency brazing. At one time, they have lower cost and reduced dosage.

结果表明：三种预合金粉末有着优良的低温烧结性能和耐磨性，表现出接近于超细钴和相当于国外代钴粉末的优良的物理、机械性能，对金刚石把持力高；具有高的高频钎焊结合力矩；粉末成本较低，用粉量可降低。

METHODS: NSCs were isolated from neonatal rats by enzyme digestion and mechanical separation. At the fourth passage, cell clone masses received nestin immunocytochemistry. Remaining cells were dispersed by mechanical separation. Monoclone NSCs were incubated by limiting dilution assay, and made into 108 L-1 monoplast suspension in complete medium. NSCs were assigned into 2 groups. NSCs in the control group were incubated in 10% fetal bovine serum.

酶消化和机械分离法相结合体外分离培养新生鼠神经干细胞，传至第4代的细胞克隆团行巢蛋白免疫细胞化学染色观察，剩余细胞团用机械法分散，采用有限稀释法进行单克隆神经干细胞培养，加入完全培养基制成108 L-1的单细胞悬液，分为2组滴入培养板，对照组加入10%FBS，诱导组加入10%FBS+50 μg/L神经生长因子，培养5~7 d。

When reached logarithm growth phase, it was poured or the medium was removed. 5 mL medium containing 10 mg/L mitocin-C was added , kept at 37 ℃, and then cultured in saturated humidity warm box with the CO2 of 0.05 fraction volume for 2 or 3 hours. It was coated with 0.1% gelatin in 6-hole plate, laid for over 30 minutes, and then threw away or the medium with mitocin-C was removed, washed with phosptat buffer for 5 times so as to remove the mitocin-C. 2 mL 0.05% trypsin digestive cells were added, and it was observed under microscope. When crevice appeared, cells became round (about 2－4 minutes), digestion was stopped by adding medium of the same volume, and blew up with straws repetitively to make it into monoplast suspension. Special-used cover glass was put in the center of cell counting chamber. Cells were sucked in by glass siphon, and cell suspension flew out at bucket of up or down-sides of counting chamber, until the cover glass was filled with fluid. Living cell were inoculated at concentrations of 3×108, 5×108, 1×109 L－1 after their number counted.

选取2～5代的小鼠胚胎成纤维细胞，待其至对数生长期倒掉或吸掉培养基，加入含10 mg/L丝裂霉素C的细胞全培养液5 mL，置37 ℃，体积分数0.05的CO2饱和湿度温箱中培养二三小时，在6孔板中加入0.1%明胶包被，放置30 min以上，倒掉或吸掉含丝裂霉素C的培养基，磷酸盐缓冲液清洗5遍，尽量除去丝裂霉素C，加入2 mL 0.05%的胰蛋白酶消化细胞，镜下观察，当细胞间出现裂隙，细胞变圆时（约2～4 min），立即加入等量的全培养液终止消化，并用吸管反复吹打，使之成为单细胞悬液，在细胞计数板中央放置专用的盖玻片，用玻璃虹吸管吸取细胞，让虹吸管在盖玻片上或下侧的计数板凹槽处流出悬液，至盖玻片下被液体充满为止。

Seen from clinical effect, haemodynamics and general condition both better in rats treated with dexamethasone than rats treated with saline. We had a presumption on these grounds that the sum of negativity myodynamia generated byβ3-AR up-regulation andα1-AR down-regulation possibly less than positive myodynamia generated byβ1-AR up-regulation. 5 For patients with DCHF, adding prednisone to the usual care resulted in significant relief of the congestive symptoms, which was accompanied with a potent diuresis with time and an improvement in renal function.

从临床效果来看，地塞米松治疗的心衰大鼠在血流动力学及一般状况上，都明显优于注射生理盐水的心衰大鼠，据此推测，β3受体上调和α1受体下调产生的负性肌力之和可能弱于β1受体上调产生的正性肌力。5对于失代偿的充血性心力衰竭病人，在心衰常规治疗基础上加用强的松这种糖皮质激素能显著缓解充血症状，并伴随着强有力的利尿和改善肾功能的作用。

Unitary linear regression is done between w~e、 w~ρ、 e~ρ、w_L~w_P. For α_v 、 C_ c between w、ρ、 e、 w_P in eastern China, unitary linear regression is done to silty clay as well as multivariant regression with coefficient iteration method and least-squares procedure are done to all kinds of soil, and the two results are compared. Unitary linear regression and multivariant regression are done to c_q、φ、 c_d、φ_d between e and I_P. Unitary linear regression and multivariant linear regression are done to napierian logarithm of q_u between e 、 I_L、 w_P and w_L, then the equations of regression are checked with other parameters.

对物理指标含水量～孔隙比、含水量～湿密度、孔隙比～湿密度、液限～塑限作了一元线性回归；对华东地区粉质粘土的压缩系数、压缩指数和含水量、湿密度、孔隙比及塑性指数作了一元线性回归；对华东地区所有土类的压缩系数、压缩指数和物理性质参数用系数迭代法和最小二乘法作了多元线性和非线性回归，并对两种方法得到的回归结果作了比较；作了快剪强度指标和固结快剪强度指标关于孔隙比和塑性指数的一元线性回归和多元线性及非线性回归；对无侧限抗压强度的自然对数值和孔隙比、液性指数、液限及塑限作了一元和多元线性回归，并用其它工程的参数对回归方程进行了检验。

A dynamic generating toxic gas system and a nose-only exposure system were used for the pharmacokinetic study of inhaled environmental contaminants for benzene, toluene, xylene, ethylbenzene,chlorobenzene, styrene, isopropyl benzene, tetrachloroethylene, nonane and methylcyclohexane in male guinea pig.

用动态配气设备和鼻式吸入方式染毒，采用雄性豚鼠对环境中10种挥发性污染物(苯、甲苯、对二甲苯、乙基苯、氯代苯、苯乙烯、异丙苯、四氯乙烯、甲基环己烷和壬烷混合物)的吸入动力学进行了研究。

S-Naproxen was obtained by asymmetric cross-coupling reaction of 2-bromo- 6-methoxynaphthalene with organozinc reagent of ethyl α- bromopropionate, catalyzed by chiral β-aminoalkylphosphine-nickel complexes.

用β -氨基烷基膦的NiCl2 配合物为手性催化剂，以 6 -甲氧基- 2 -溴萘和α-溴代丙酸酯的有机锌试剂为原料，通过不对称交叉偶联反应合成S --萘普生。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐