代用

Formylation and diformylation of dithieno [2,3-b:3',2'-d] thiophene were prepared by two methods. Firstly, aromatic carbanions were prepared by 1 Br/Li exchange of bromo dithieno[2,3-b:3',2'-d]thiophene with n-BuLi in THF at low temperature; 2 deprotonation of dithieno[2,3-b:3',2'-d]thiophene with LDA.

二噻吩并[2，3-b：3'，2'-d]噻吩单醛与双醛的制备首先通过两种方法产生芳基负离子：1溴代并三噻吩在四氢呋喃中与正丁基锂发生的溴锂交换；2用LDA对二噻吩并[2，3-b：3'，2'-d]噻吩去质子化作用。

It is especially suitable to deal with the complicated nonlinear problem-complex structure and property relations. In this paper it is applied to solve the fungicidal activity of aseries...

用该法处理硫代磷酰胺酯类衍生物杀菌作用的构效关系，并与逐步回归和偏最小二乘法作比较，模型的复相关系数R和剩余标准差Sy都较好。

A new aroma compound,furfuryl thiopropionate was synthesized using furfuryl thioalcohol and propionyl chloride as raw materials,with calcium oxide and sodium carbonate as acid-capturer.

以糠硫醇和丙酰氯为原料，用氧化钙和碳酸钠为复合缚酸剂，合成了香料硫代丙酸糠酯，并用IR、元素分析等进行了表征。

The third passage cells had been induced by 0.1 μmol/L dexamethasome, 10 mmol/L β glycerophosphate and 50 μmol/L ascorbic acid 2 phosphate.

取第3代培养细胞用含0.1 μmol/L地塞米松、10 mmol/L β甘油磷酸钠、50 μmol/L维生素C的成骨细胞分化培养液培养。

On the 1st and 7th days, supernatant was derived from cells in primary culture to measure content of β-glycerophosphate disodium with chemiluminescence technique.

取原代培养细胞1和7 d时的上清液，用化学发光法测定β-HCG含量。

The shortage of this way was that feeder cells could be passaged together with hESCConclusion HAFi could replace MEF as feeder layer to support the long-term culture oftwo Chinese human embryonic stem cell lines. This could remove the cells of animal originand heterogenetic pollution in the culture system. It also reduced the culture of primarycells and solved the instability caused by different batches of primary cells.

结论HAFi细胞可以用做两株中国人胚胎干细胞培养的饲养层，取代MEF后解决了动物源性细胞混杂和异源污染问题，省却了饲养层原代细胞的培养步骤同时解决了由不同批次饲养层差异带来的培养体系的不稳定。

We also used a hyperboloidal panel to eliminate the g...

Newton-Raphson迭代过程被用来在桨叶随边满足压力Kutta条件。

The materials kept in hypothermal disinfectant Co60 were heated to 37℃ by waterbath after surface treatment, and then placed in a 6-well culture plate. SMC suspension was added into the middle of the materials.

将培养所得第6代平滑肌细胞用0.25%胰蛋白酶在室温下消化3 min，加入10%牛血清BMEM培养液10 mL，制成平滑肌细胞悬液。

Mixed finite element equation with u〓-u〓-p variables has indefinitive coefficient matrix and therefore can not be solved using direct methods and traditional iterative methods.

基于〓变量的混合有限元法，系统方程的系数矩阵非正定，不能用直接法和传统的迭代法求解。

We introduced improved primary mixed glial culture and different-attachment method to isolate and purify the OPCs, the cells were proliferated in serum-free medium, flow cytometry and immunohischemistry methods were employed to estimate the purity of cultured OPCs. Their abilities of differentiation and expression of trophic factors were identified by RT-PCR and immunostaining. Several methods including TUNEL and MTT were adopted to estimate the protective effects of conditioned culture medium from oligodendrocyte lineage cells on the primary cultured cerebellar granule neurons. Intravitreal transplant of OPCs, combined with retrograde fluorescent labeling the superior colliculus and intraorbital optic nerve transection, were used to investigate the protective effects of OPCs on the axotomized RGCs in vivo. Intravitreal transplantof OPCs or NSCs on the newborn rats, and retinal transplant of OPCs on the young rats were performed, to observe the myelin formation in the retina at different stages after cellular transplantation. Optic nerve transection was carried out on some rats with myelinated retinae, to study the influence of myelination on the injuried RGCs.

为此，本研究采用改良的胶质细胞混合培养与差速贴壁方法获得大鼠OPCs，使用无血清培养基进行扩增、培养，用免疫组织化学和流式细胞技术对培养细胞的纯度进行鉴定，对少突胶质系细胞表达部分营养因子的情况进行检测；采用TUNEL、MTT等方法对少突胶质系细胞条件培养基对原代培养小脑颗粒神经元的保护作用进行检测；将OPCs移植入成年SD大鼠玻璃体内，利用上丘逆行荧光标记技术，观察眼内移植的OPCs对眶内视神经切断时的视网膜神经节细胞的保护作用及其持续时间；将OPCs或NSCs移植入新生和幼年SD大鼠玻璃体或视网膜内，观察不同时期视网膜内髓鞘形成与分布特点，分析髓鞘的超微结构，并观察眼内髓鞘形成对损伤神经节细胞的保护作用。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐